A kit for simultaneous detection of avian leukemia virus antibodies and Salmonella pullorum antibodies

A technology for avian leukosis and pullorum, applied in the biological field, can solve the problems of not being able to detect avian leukaemia and pullorum at the same time, requiring a lot of manpower and cost, and achieve the effect of reducing the work of clinical detection and purification
CN108776221BActive Publication Date: 2020-07-31CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-07-31

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Abstract

The invention relates to the technical field of biology and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. An antigencombination of the kit comprises p27 protein, gp85 protein and GroEL-delta8-1 protein. Avian leukosis virus capsid protein p27, prokaryotic expression protein of envelope protein gp85, and prokaryoticexpression protein of truncated GroEL-delta8-1 of a salmonella pullorum dominant antigen are used as a coating antigen to develop an ELISA (Enzyme-linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorum antibody. Compared with a current common kit for independently detecting avian leukosis or pullorum disease, the kit provided by the invention has the effect of simultaneously detecting the avian leukosis or the pullorum disease, and the detection and purification work of the avian leukosis and the pullorum disease can be extremely alleviated.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a kit for simultaneous detection of antibodies to avian leukosis virus and Salmonella pullorum. Background technique

[0002] Avian Leukosis is a general term for a variety of tumor diseases in poultry caused by viruses in the Avian Leukosis Virus (ALV) and Avian Sarcoma Virus (ASV) groups. The disease can cause many infectious benign and malignant tumors in chickens.

[0003] Since Roloff reported chicken lymphosarcoma in 1868, avian leukemia has been distributed all over the world, and natural cases have also occurred in my country. The disease can cause slow weight gain, delayed sexual maturity, small eggs, thin eggshells, decreased egg production, low fertilization rate and hatchability, increased carcass waste rate, and increased mortality, causing direct economic losses. At the same time, it can also cause the body's non-specific resistance to decrease and immunosuppression, ...

Claims

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