71 results about "Salmonella Brijbhumi" patented technology

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention discloses a detection kit and a detection method for 9 species of pathogenic organisms in marine products. The kit comprises Taq DNA polymerase with a concentration of 5U / muL and a PCR reaction solution, wherein the PCR reaction solution contains 10 millimols of Tris.HCl, 50 millimols of KCl, 25 millimols of MgCl2, 2.5 millimols of dNTP and primer pairs of the 9 species of pathogenic organisms. The kit and the method can synchronously detect the 9 species of pathogenic organisms and can detect salmonella with a concentration of 11 CFU / mL, staphylococcus aurei with a concentration of 2,000 CFU / mL, monocytogenes with a concentration of 120 CFU / mL, enterohemorrhagic Escherichia coli O157:H76 with a concentration of CFU / mL, comma bacillus with a concentration of 220 CFU / mL, Vibrio parahaemolyticus with a concentration of 60 CFU / mL, Vibrio vulnficus with a concentration of 9 CFU / mL and Vibrio alginolyicus with a concentration of 230 CFU / mL. The detection method short in detection time and simple and quick in operation, and can save a large amount of labor and financial resources and meet requirements for quick detection.

Methods, compositions and bacterial isolates for improving the gastrointestinal health of animals and in particular of poultry are provided herein. The methods include administering an endospore-forming bacteria to an animal. The bacteria are selected for the ability to reduce the growth and presence of bacterial pathogens, such as Salmonella, Clostridium, and Campylobacter, in the gastrointestinal tract of the animal. The bacteria are also selected for the ability to improve at least one production parameter in the animal.

An antimicrobial, anti-bacterial processing aid, food additive and food ingredient is provided to inhibit cellular growth of known pathogenic, indicator and spoilage organisms, such as salmonella, stahphylococcus, listeria, e coli, and the like. The antimicrobial agent of the present invention is useful as a treatment for animal feed, a treatment for pre-harvest and post-harvest processing of foodstuffs, a treatment for cooked food subject to airborne contaminants and many other conditions in need of disinfectants and sanitizers. One of the primary benefits of the antimicrobial agent is that it inhibits the growth of bacteria that have become antibiotic resistant.

The invention provides an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide is obtained through rational molecular design on the basis of antibacterial peptide Indolicidin separated from bovine neutrophils; and the antibacterial peptide is antibacterial peptide Indo-1 with the amino acid sequence shown as SEQ ID NO. 1 or antibacterial peptide Indo-2 with the amino acid sequence shown as SEQ ID NO. 2. The antibacterial peptide provided by the invention has good structural characteristics; the antibacterial effect on staphylococcus aureus, salmonella and aspergillus flavus can be obviously improved; and meanwhile, the hemolysis rate and the cytotoxicity of chicken red blood cells can be effectively reduced; the antibacterial peptide can be effectively used in animal-related production activities.

The invention relates to a Bifidobacterium lactis strain J605 and an application thereof, and belongs to the technical field of microorganisms. The preservation number of the Bifidobacterium lactis strain J605 is CGMCC (China General Microbiological Culture Collection Center) No.21751. The strain J605 has a good intestinal tract probiotic function and relatively high gastrointestinal tract stress resistance, has an obvious inhibition effect on escherichia coli, staphylococcus aureus and salmonella, has sensitivity to common antibiotics, and can be used for preparing a feed additive for preventing and treating intestinal diseases. The compound can be widely applied to medicines, health-care foods or drinks for improving the intestinal environment, and has high market application value.

The invention relates to the technical field of probiotics and particularly relates to lactobacillus reuteri and an application thereof. The invention provides Lactobacillus reuteri SLZX19-12 which ispreserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Lactobacillus reuteri SLZX19-12 is CGMCC No. 20121. Lactobacillus reuteri SLZX19-12 can be planted in an animal body, and is high in acid resistance and cholate resistance; the good inhibition effect is achieved on escherichia coli and salmonella; feruloyl esterase can be generated, and release and digestion of fibers in the feed in digestive tracts are promoted; the lactobacillus reuteri SLZX19-12 is prepared into a microbial inoculum and then fed to animals, intestinal floracan be improved, intestinal immunity can be improved, intestinal health can be improved, and the effect is safe and reliable.

The invention discloses fluorescent quantitative polymerase chain reaction (PCR) primers and probes for detecting pathogenic bacteria of multiple aquatic products simultaneously and a detection method, and belongs to the field of molecular detection. Five groups of the fluorescent quantitative PCR primers and the probes are provided and can be used for detecting salmonella, listeria monocytogenes, vibrio parahaemolyticus, shigella flexneri and vibrio cholerae non-O1 respectively, wherein except the primers and the probes for listeria monocytogenes genes and the primers and the probes for vibrio parahaemolyticus genes cannot be used simultaneously, the pathogenic bacteria which can be detected by the corresponding primers and probes can be detected simultaneously by combining any other two groups or more than two groups of the primers and probes. The invention also discloses multiple fluorescent quantitative PCR amplification systems which are constructed by using the primers and the probes, so the primers and the probes can detect whether samples to be detected contain the corresponding pathogenic bacteria or not quickly and accurately, are convenient to operate and high in specificity, can be made into kits and the like and are applicable to the detection of the pathogenic bacteria of the subsidiary agricultural products, and a false positive result is avoided.

The invention discloses a bacteriophage lysin with animproved antibacterial effect. The 99th leucine and the 102nd methionine of lyase Bp7e amino acid are respectively mutated to obtain alanine and glutamic acid, by using a prokaryotic expression technique, mutant protein can be obtained, and the mutant protein is identified by using Western-blot and is named Bp7c mutant protein. Bp7e and Bp7e mutant protein are purified, the concentration of Bp7e and Bp7e mutant protein is detected, and the in-vitro pyrolysis experiment and the pyrolysis spectrum detection show that purified Bp7e and Bp7e mutant protein have a broad-spectrum antibacterial function and have pyrolysis effects on micrococcus lysodeikticus, staphylococcus aureus, salmonellae and multiple serotype escherichia colis, and the pyrolysis effect of the Bp7e mutant protein is wholly superior to that of natural lyase Bp7e.

The invention relates to growth-inhibitory composition against pathogenic bacteria of meat based food stuff comprising IgY. The IgY is a specific immunoglobulin derived from yolk of egg. 12 representative bacteria and other microbes deteriorating quality of process meat products that the IgY of the invention targets include Aeromonas hydrophila, Bacillus cereus, which comprises Aeromonas hydrophil, Bacillus cereus, Camphlobacter jejuni, Clotridium perfringens, 0157:H7 (Escherichia col, 0157:H7), Lactobacillus, Listeria monocytogens, Saccharomyces cerevisiae, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis. The 12 representative bacteria antigen forms are respectively injected the interior of chicks to obtain the IgY of the 12 antigens.

The invention discloses a medical silver-carrying antibacterial agent modified PP material and a preparation method thereof. The PP material is prepared through the following steps: weighing the components of PP, EPDM, a nanometer silver-carrying zirconium phosphate antibacterial agent, barium stearate, acetone, talcum powder, an antioxidant, titanium dioxide, dicumyl peroxide, butadiene rubber, a surface treating agent, lead stearate, propylene, zinc oxide and 5-ethylidene-2-norbornene in parts by weight, uniformly mixing the weighed components, and extruding and pelleting the uniformly-mixed components so as to obtain the PP material. The PP material has the advantages that the breaking elongation rate is 15-55%, the tensile strength is 28-30 MPa, the antibacterial rate for escherichia coli is 98-99%, the antibacterial rate for golden staphylococcus is 97.5-99.5%, the impact strength is 3-5 kJ / m<2>, the bactericidal rate for pseudomonas aeruginosa is 99-99.5%, and the bacteriostasis rate for salmonella typhimurium and Klebsiella pneumoniae is 92-96%.

The invention relates to the technical field related to elimination of thalli, and particularly provides a preparation for strongly eliminating vibrios and a preparing method of the preparation. On one hand, provided is the preparation for strongly removing vibrios, wherein the preparation comprises the components in percentage by weight: 65-85% of a first guanidine derivative, 2-12% of a second guanidine derivative and the balance being ethanol, wherein the first guanidine derivative is polyhexamethylene guanidine salt and / or polyhexamethylene biguanide salt; and the relative molecular weightof the second guanidine derivative is 500-1500. The vibrios can be effectively removed by utilizing polyhexamethylene guanidine hydrochloride and chlorhexidine hydrochloride under the specific content. The preparation provided by the invention has a strong removal effect on pathogenic vibrios, such as vibrio parahaemolyticus, vibrio anguillarum, vibrio alginolyticus, vibrio cholerae, vibrio fluorescens and the like, has a good cracking effect on aeromonas hydrophila, aeromonas punctata, escherichia coli, salmonella and other gram-negative bacteria, and has a quite high removal rate after being used for 48 hours.

The invention belongs to the technical field of research and development on medical products. The invention applies for the protection of a method for extracting components having antibacterial activity from fruit of pedunculate herpetospermum which is a Tibetan medicine. The method comprises: extracting coarse extract from the dried and crushed fruit of pedunculate herpetospermum by using a solvent and a solid-liquid extraction method, and concentrating the coarse extract to obtain extract; obtaining extract with obvious antibacterial effect by degreasing, concentrating and extracting; and further separating the extract by silica gel column chromatography and obtaining the effective extract components. The product of the invention has different inhibition effects on golden staphylococcus, tritirachium album, bacillus subtilis and salmonella, can be developed as a natural antibacterial to be used in medical industry and can be prepared into antibacterial tablets, granules, capsules, injection, skin external preparations and other formulations. Thus, a new field of the development of the pedunculate herpetospermum fruit Tibetan medicine is opened.

The invention discloses a non-oxidized type compound bactericide for water treatment and a preparation method thereof. The non-oxidized type compound bactericide is prepared by the steps of weighing glutaraldehyde, acrylic acid-2-acrylamide-2-methyl propane sulfonic acid copolymer, tetradecyl benzyl dimethyl ammonium chloride, etidronic acid, water, PBTCA, a polymerization inhibitor, sulfonic acid, polyol phosphonate ester, PESA, a dispersant, sodium tungstate, bitertanol, sodium benzoate and acrylic acid-hydroxy-propyl acrylate copolymer in parts by weight, and mixing uniformly. The product is 100% water-soluble, and has a sterilizing rate of 99.9-100% to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium notatum, aspergillus, saprophytic bacteria and salmonella; and the sterilizing rate is 98-100% after the bactericide is placed for one month at 50-60 DEG C, the sterilizing rate is 97.9-99.9% after the bactericide is placed for half a year at 40-50 DEG C, and the sterilizing rate is 97.5-99.5% after the bactericide is placed for 1-2 years at room temperature.

The invention relates to the use of lysozyme and restructured lysozyme 134 pure product or stock solution in preparing pharmaceutical preparations for the prevention and treatment for birds including chickens, ducks, geese, quails and livestock including pigs, sheep, cows, horses, donkeys, rabbits and honey bees. Wherein the restructured lysozyme 134 pure product or the combination of stock solution and antibiotics are employed to treat the diseases caused by viruses, Staphylococcus aureus, streptococcus, bacillus coli, salmonella, mycoplasma and Chlamydia.

The invention discloses a compound micro-ecological preparation for preventing and treating necrotic enteritis of poultry and application of the compound micro-ecological preparation. The compound micro-ecological preparation is compounded by taking bacillus velezensis, lactobacillus plantarum and xylooligosaccharide as active ingredients. The bacillus velezensis and lactobacillus plantarum strains have a relatively strong inhibition effect on intestinal pathogens such as clostridium perfringens, salmonella and escherichia coli, and are combined with the intestinal probiotic function of xylooligosaccharide, so that the intestinal health of poultry can be adjusted, necrotic enteritis of poultry can be effectively prevented and treated, and the use of antibiotics in the breeding process is reduced.

The invention discloses applications of Phyllanthus emblica or Phyllanthus emblica and a Phyllanthus emblica composition in preparation of products for preventing and / or treating at least one of mastitis, diarrhea and porcine respiratory diseases, and further provides antibacterial effects of Phyllanthus emblica or Phyllanthus emblica extract and the Phyllanthus emblica composition, wherein the Phyllanthus emblica or Phyllanthus emblica extract and the Phyllanthus emblica composition can effectively inhibit the growth of one or a variety of bacteria selected from Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas, Salmonella choleraesuis, Escherichia coli, Group A beta-hemolytic streptococcus, Streptococcus suis, Bordetella, Pasteurella multocida, Salmonella enterica, Staphylococcus haemolyticus, Enterococcus faecalis and Erysipelothrix rhusiopathiae, and the effectiveness of the uses are proved through experiments so as to expanded the applications of Phyllanthus emblica andthe Phyllanthus emblica composition.

The invention belongs to the technical field of biology, and discloses pichia anomala as well as a fermentation culture and application thereof. The pichia anomala is pichia anomala AR2016, CCTCC NO:M2017594. The fermentation culture medium provided by the invention is used for liquid state fermentation; the biomass of the fermentation liquid is 15g / L; the yield of the Killer toxin is 5.86mg / L; the yield of beta-1,3-glucanase is 964 U / L; the yield of phytase is 1675 U / L. The pichia anomala provided by the invention has the effects of inhibiting dysentery colibacillus, salmonella pullorum, sensitive C type candida albicans, actinobacillus pleuropneumoniae, sarcina lutea and staphylococcus aureus and resisting gastric acid and bile salts. Therefore the pichia anomala provided by the invention can be used as feed additives for livestock, poultry and aquatic animals; wide application prospects are realized.

A chicken meat product rendered substantially free of pathogenic bacterial species by having an external coating of sodium diacetate substantially free of an adhesive material. The wet coating is retained on the external surfaces of the dressed chicken meat by the surface tension of the sodium diacetate solution after the chicken meat is coated with the solution and is hung freely to permit excess liquid to drain away. The amount of sodium diacetate present in the product of the invention depends upon the degree of pathogenic bacterial contamination of the chicken meat. The sodium diacetate is included in each case in sufficient quantity to preserve the meat from growth of all pathogenic bacteria of the order and species of salmonella, E. coli, and campylobacter.

The invention specifically relates to a Bacillus subtilis strain and a method for preparing Pu'er tea capable of regulating intestinal functions from the same, belonging to the field of microbes and food biotechnology. The Bacillus subtilis strain has been preserved in China General Microbiological Culture Collection Center, with an accession number is CGMCC No. 15959. The Bacillus subtilis strainof the invention is applied to the processing technology for Pu'er tea, and can effectively inhibit the growth of other harmful microorganisms, including Staphylococcus aureus, Salmonella, Aspergillus flavus and other toxin-producing fungi. The Pu'er tea prepared from the Bacillus subtilis strain is capable of regulating intestinal functions.

The invention discloses a production method of ultrahigh-pressure cold-sterilized cow milk, which sequentially comprises the following steps: (1) detecting raw cow milk, and filling the raw cow milk which is detected to be qualified within 6 hours after collection to obtain packaged cow milk; (2) performing ultrahigh-pressure treatment on the packaged cow milk obtained in the step (1) to obtain sterilized cow milk; and (3) refrigerating and storing the sterilized milk obtained in the step (2) at 2-6 DEG C, and transporting and selling the sterilized milk at 2-6 DEG C, wherein the detection standards in the step (1) are as follows: the total number of bacterial colonies is less than or equal to 5000CFU / mL, the number of somatic cells is less than or equal to 200000 / mL, and escherichia coli,staphylococcus aureus and salmonella cannot be detected every 25mL. The invention also discloses the ultrahigh-pressure cold sterilization cow milk prepared by the method. According to the invention,the raw milk is sterilized by adopting ultrahigh-pressure cold sterilization, and through reasonable raw material control and process design, the fresh flavor and nutritional value of the milk are reserved to the greatest extent on the premise of ensuring food safety.

The invention discloses a quadruple fluorescence PCR method for detecting four types of pathogenic bacteria. The four types of pathogenic bacteria are staphylococcus aureus, salmonella enterica, listeria monocytogenes and vibrio parahemolyticus. Four elements, namely sample DNA, a pair of universal primers and four probes, fluorescence PCR premix liquid and positive control are adopted in the method. The method comprises the following steps: designing the primers and the probes in accordance with 16S rRNA genes of the four types of bacteria, implementing amplification on a target sequence and exciting corresponding fluorescence signals; and preparing a kit from the to-be-detected sample DNA in accordance with a reagent formula and amplification conditions of the fluorescence PCR amplification; and specifically, the method comprises the following steps: establishing a quadruple fluorescence PCR primer system for detecting the four types of bacteria; establishing the quadruple fluorescence PCR kit for detecting the four types of bacteria; and determining the quadruple fluorescence PCR identification method for detecting the four types of bacteria. The method provided by the invention has the advantages of being low in standard error, short in detection time, and being capable of simultaneously detecting a plurality of target genes and reducing reagent cost; and the method is applicable to detection of pathogenic bacteria in food and cosmetics.

The invention discloses a preparation method of a dogwood seed extract. The preparation method comprises the following steps: a, crushing dried dogwood seeds into ultrafine dogwood seed powder; b, adding absolute ethyl alcohol into the ultrafine dogwood seed powder, and carrying out soaking for 24 hours at a temperature of 50 DEG C; and c, putting a soaking solution into a rotary evaporator, carrying out reduced-pressure distillation, and performing drying to obtain a crude dogwood seed extract. The crude dogwood seed extract has a good antibacterial effect on escherichia coli and salmonella,and has no obvious cytotoxicity on rabbit red blood cells. According to the preparation method of the dogwood seed extract, only one-step extraction through ethyl alcohol is needed, extraction time isshort, extraction efficiency is high, the obtained effective components of dogwood seeds are high in amount, and the influence of experimental conditions on extraction is small.

The invention provides a preparation method and application of a bombyx mori antibacterial peptide BMGlvA2 recombinant protein, and the amino acid sequence of the bombyx mori antibacterial peptide BMGlvA2 recombinant protein is as shown in SEQ ID NO. 1. The invention relates to the field of gene engineering, and an engineering strain Trichoderma reesei is used as an expression host to realize expression of the bombyx mori antibacterial peptide BMGlvA2 recombinant protein. Escherichia coli, salmonella and clostridium perfringens are used as indicator bacteria at the same time for the first time, and it is identified that the antibacterial peptide fermentation product have obvious antibacterial activity on the indicator bacteria. The invention also characterizes the self properties of temperature tolerance, digestive enzyme tolerance, hemolytic activity and the like of the antibacterial peptide, and also preliminarily provides an inhibition mechanism of the antibacterial peptide on clostridium perfringens. The bombyx mori antibacterial peptide BMGlvA2 provided by the invention is beneficial to further development and application in the field of feeds.

The invention relates to additive premixing feed, and belongs to the field of agricultural microbial feed additives. The feed additive contains lactobacillus plantarum WLPL01 and a culture obtained after Artemisia argyi Levl. et Van is fermented by the lactobacillus plantarum WLPL01. The lactobacillus plantarum WLPL01 fermentation culture has an antibacterial function for escherichia coli O157: H7, Listeria monocytogenes, staphylococcus aureus, Monilia albican and salmonella typhimurium. On a premise that the feed additive is not harmless to animals, the quality of the feed can be improved, afeed use ratio is improved, animal immunity can be effectively enhanced, the infection of the salmonella typhimurium is prevented, and the additive premixing feed has an important meaning for accelerating the sustainable development of breeding industry and guaranteeing product quality safety.

The invention discloses clostridium butyricum with strong bacteriostatic ability and application of the clostridium butyricum. The clostridium butyricum is preserved in China General Microbiological Culture Collection Center on April 29, 2021, and the biological preservation number of the clostridium butyricum is CGMCC NO.22258. The clostridium butyricum screened in the invention does not have clostridial toxin and botulinum toxin genes, is narrow in drug resistance spectrum, high in safety and high in tolerance to gastrointestinal fluid, cholate and high temperature, has the characteristic of high bacteriostatic ability to pathogenic bacteria such as escherichia coli, salmonella, morganella morganii and klebsiella pneumonia, and has good application potential for animal feed probiotics.

The invention discloses a feed additive for preventing calf diarrhea as well as a preparation method and an application thereof, and the feed additive for preventing calf diarrhea is composed of the following components in percentage by weight: 5-50% of organic acid, 1-30% of terpene, 2-10% of plant polyphenol and 10-60% of an inert carrier. According to the invention, the synergistic interactionof various antibacterial substances such as organic acid, terpene, plant polyphenol and the like is utilized; escherichia coli and salmonella in feed raw materials and livestock and poultry bodies areeffectively inhibited, the intestinal microecological balance of the calves is maintained, accordingly, diarrhea of the calves is prevented, the feed additive has the advantages of being high in safety and free of drug resistance, antibiotics can be effectively replaced, the diarrhea occurrence rate is reduced, growth of the calves is promoted, and the feed additive conforms to the development direction of healthy breeding.

The invention provides a goose source lactobacillus plantarum ER41 (Lactobacillus plantarum ER41), and the preservation number of the goose source lactobacillus plantarum ER41 is CCTCC (China Center for Type Culture Collection) No:M 2021932. The lactobacillus plantarum ER41 has the following technical effects: 1) the lactobacillus plantarum ER41 can quickly produce acid, the pH value in 8 hours is reduced to 4.0 or below, the lactic acid yield in 24 hours is 165.87 mmol / L, and the lactobacillus plantarum ER41 is quick in acid production and strong in lactic acid production capacity; 2) the antibacterial effect on escherichia coli, staphylococcus aureus, salmonella and listeria monocytogenes is achieved; 3) the strain can more effectively adhere to epithelial cells of the goose intestinal tract and colonize in the goose intestinal tract; 4) the villus height of the jejunum, the ileum and the cecum can be increased, the crypt depth of the ileum and the cecum can be obviously reduced, the villus-implicit ratio of the cecum can be obviously increased, and the healthy development of intestinal mucosa can be promoted; 5) the content of acetic acid, propionic acid and other short-chain fatty acids in the intestinal tract can be increased, and the intestinal tract health is promoted; and 6) the abundance of escherichia coli shigella, enterococcus and other pathogenic bacteria can be remarkably reduced, the abundance of lactobacillus is increased, and the intestinal flora is adjusted.