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Compound micro-ecological preparation for preventing and treating necrotic enteritis of poultry and application of compound micro-ecological preparation

A technology of complex microecology and necrotic enteritis, applied in the field of feed, can solve the problem of Bacillus velesii that has not yet been found

Active Publication Date: 2021-07-27
TIANJIN CHIATAI FEED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Lactobacillus plantarum, Lactobacillus salivarius, Bifidobacterium, etc. have been reported to have antibacterial activity against Clostridium perfringens. enteritis reports

Method used

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  • Compound micro-ecological preparation for preventing and treating necrotic enteritis of poultry and application of compound micro-ecological preparation
  • Compound micro-ecological preparation for preventing and treating necrotic enteritis of poultry and application of compound micro-ecological preparation
  • Compound micro-ecological preparation for preventing and treating necrotic enteritis of poultry and application of compound micro-ecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Screening and Identification of Clostridium perfringens, Salmonella and Escherichia coli Antagonistic Species

[0039] 1 Material method

[0040] 1.1 Test material

[0041] 1.1.1 Strains

[0042] Clostridium perfringens, Salmonella enterica subsp. enterica and Escherichia coli were purchased from China Industrial Microorganism Culture Collection Center, and probiotic strains were isolated and preserved by Beijing Keweibo Biotechnology Research Institute.

[0043] 1.1.2 Medium

[0044] (1) Tryptone-sulfite medium

[0045] Tryptone 15.0g, soybean peptone 5.0g, yeast powder 5.0g, sodium metabisulfite 1.0g, ferric ammonium citrate 1.0g, pH7.6±0.2, sterilize for 30min.

[0046] Solid medium: add 1.6% agar powder on the basis of liquid medium.

[0047] (2) LB medium

[0048] Tryptone 10.0g, yeast extract powder 5.0g, sodium chloride 10.0g, add distilled water to 1000mL, pH7.0±0.1, sterilize at 121°C for 30min.

[0049] Solid medium: add 1.6% agar powder on the ...

Embodiment 2

[0079] The preparation of embodiment 2 compound probiotics

[0080] 1. the production method step of bacillus velei is as follows:

[0081] (1) Inoculate the Bacillus veleus species on the slant medium, and cultivate it at 30-40°C for 12-24 hours;

[0082] (2) Inoculate the Bacillus Velez cultured on the slant into the seed activation liquid medium, and cultivate it for 12-24 hours under the conditions of 30-40°C and 160-220rpm;

[0083] (3) Inoculate the Bacillus veleus seed solution in the seed tank with a weight ratio of 0.5%-2%, and cultivate it for 12-24 hours at 30-40°C and 160-220rpm;

[0084] (4) Inoculate the seed tank culture solution in the fermenter with a weight ratio of 0.5%-2%, the tank pressure is 0.01-0.05MPa, the stirring speed is 160-220rpm, and the ventilation ratio is 0.5-1:0.1 in terms of volume ratio -0.5, the fermentation time is 16-24 hours;

[0085] (5) Spray-dry the Bacillus velei fermented liquid through a drying tower, the air inlet temperature ...

Embodiment 3

[0098] Example 3 Effects of Compound Probiotics on Broiler Growth Performance and Intestinal Flora

[0099] 1 test material

[0100] Bacillus Velez, Lactobacillus plantarum and compound microecological preparations were produced and provided by Beijing Keweibo Biotechnology Co., Ltd., and Clostridium perfringens was purchased from China Industrial Microbiology Culture Collection Center.

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Abstract

The invention discloses a compound micro-ecological preparation for preventing and treating necrotic enteritis of poultry and application of the compound micro-ecological preparation. The compound micro-ecological preparation is compounded by taking bacillus velezensis, lactobacillus plantarum and xylooligosaccharide as active ingredients. The bacillus velezensis and lactobacillus plantarum strains have a relatively strong inhibition effect on intestinal pathogens such as clostridium perfringens, salmonella and escherichia coli, and are combined with the intestinal probiotic function of xylooligosaccharide, so that the intestinal health of poultry can be adjusted, necrotic enteritis of poultry can be effectively prevented and treated, and the use of antibiotics in the breeding process is reduced.

Description

technical field [0001] The invention relates to the technical field of feed, in particular, the invention relates to the preparation and application method of a compound microecological preparation for preventing and treating poultry enteritis. Background technique [0002] Poultry necrotizing enteritis is a serious intestinal disease with acute onset and high mortality, which causes huge economic losses to poultry farming. The prevention and treatment of necrotizing enteritis in poultry production is very important. [0003] Clostridium perfringens is the main pathogenic factor causing necrotic enteritis in poultry. It is a strictly anaerobic bacterium with positive Gram staining. Under the microscope, it is a clostridium stubby bacillus with blunt ends and can form spores , spores are middle or proximal, oval in shape. Clostridium perfringens is a resident bacterium in the intestinal tract of poultry. It is a typical opportunistic pathogen and will not cause necrotic ente...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/75A23K10/18A23K20/163A61K35/747A61P1/00
CPCA23K50/75A23K10/18A23K20/163A61K35/742A61K35/747A61P1/00A23V2400/169A61K2300/00Y02A40/70
Inventor 李富伟吴培均韩明渠
Owner TIANJIN CHIATAI FEED TECH
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