Goose source lactobacillus plantarum and application thereof

A Lactobacillus plantarum, goose-derived technology, applied in the field of probiotics, can solve the problem of unresearched intestinal structure, intestinal metabolites of intestinal flora, no mention of avian intestinal cell adhesion, no mention of animal intestinal tract cell adhesion and other problems, to achieve the effect of fast acid production and strong lactic acid production ability

Active Publication Date: 2021-11-30
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application CN106399196A provides a strain of Lactobacillus plantarum HEW-A490 and its application. The bacterial strain HEW-A490 is isolated from the chicken intestine, which significantly improves the growth performance of the chicken, but does not study the effect on the intestinal structure, intestinal bacteria The role of intestinal health such as colony, and the adhesion to animal intestinal cells are not mentioned
[0006] In terms of strain adhesion research, Chinese patent application CN109504619A...

Method used

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  • Goose source lactobacillus plantarum and application thereof
  • Goose source lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Plant Lactobacillus (Lactobacillus plantarum) ER41

[0029] 1. Separation and purification

[0030] Isolate and purify Lactobacillus plantarum ER41 from goose intestinal tract, as follows: Weigh 1 g of intestinal chyme sample and add it to 99 ml of sterilized distilled water, shake it for 1 h on a constant temperature shaker, then dilute it 10 times in a gradient, and take 10 -3 、10 -4 The sample dilution solution was spread on the MRS solid medium, cultured for 48h, and then taken out, according to the size, shape and color of the colony, a single colony growing on the MRS was picked, and the catalase test and Gram staining were carried out . Catalase-negative and Gram-positive were tentatively designated as Lactobacillus, and streaked and purified on MRS solid medium for 2 times. Stored at -80°C in MRS medium with 25% glycerol, the strain was named ER41.

[0031] 2. Physiological and biochemical detection

[0032] Lactoba...

Embodiment 2

[0036] Example 2 Detection of Lactobacillus plantarum ER41 (Lactobacillus plantarum ER41) Acid Production Ability

[0037]Strain activation: inoculate the cryopreserved Lactobacillus plantarum ER41 in MRS liquid medium, culture at 37°C for 24 hours, and subculture twice in this way to obtain the activated Lactobacillus plantarum ER41; the activated strain is inoculated at a volume ratio of 3% The amount was re-transferred to MRS liquid medium, cultivated for 24 hours, and samples were taken at 4h, 8h, 12h, and 24h to measure the pH value and lactic acid production of the supernatant. The results are shown in Table 1. It can be seen from Table 1 that ER41 can produce acid rapidly, the pH value drops below 4.0 in 8 hours, and the lactic acid production in 24 hours is 165.87mmol / L, and the acid production is fast and the lactic acid production ability is strong.

[0038] Table 1: Acid production capacity of Lactobacillus plantarum ER41

[0039] time pH Lactic acid ...

Embodiment 3

[0040] Embodiment 3: the detection of antibacterial activity of Lactobacillus plantarum ER41 (Lactobacillus plantarum ER41)

[0041] Strain activation: inoculate the cryopreserved Lactobacillus plantarum ER41 in MRS liquid medium, culture at 37°C for 24 hours, and subculture twice in this way to obtain the activated Lactobacillus plantarum ER41; the activated strain is inoculated at a volume ratio of 3% The amount was retransferred to MRS liquid medium, cultivated for 24 hours, and the supernatant was obtained by centrifuging the bacterial solution.

[0042] Bacteria (Escherichia coli, Staphylococcus aureus, Salmonella, Listeria) were used as indicator bacteria, and LB medium was used as the culture medium for indicator bacteria. Activate the indicator strain preserved in glycerol. The activated strains were re-transferred into LA broth with 3% inoculum amount, and the indicator bacteria were cultured at 37°C for 24 hours, and the indicator bacteria solution was diluted with ...

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Abstract

The invention provides a goose source lactobacillus plantarum ER41 (Lactobacillus plantarum ER41), and the preservation number of the goose source lactobacillus plantarum ER41 is CCTCC (China Center for Type Culture Collection) No:M 2021932. The lactobacillus plantarum ER41 has the following technical effects: 1) the lactobacillus plantarum ER41 can quickly produce acid, the pH value in 8 hours is reduced to 4.0 or below, the lactic acid yield in 24 hours is 165.87 mmol/L, and the lactobacillus plantarum ER41 is quick in acid production and strong in lactic acid production capacity; 2) the antibacterial effect on escherichia coli, staphylococcus aureus, salmonella and listeria monocytogenes is achieved; 3) the strain can more effectively adhere to epithelial cells of the goose intestinal tract and colonize in the goose intestinal tract; 4) the villus height of the jejunum, the ileum and the cecum can be increased, the crypt depth of the ileum and the cecum can be obviously reduced, the villus-implicit ratio of the cecum can be obviously increased, and the healthy development of intestinal mucosa can be promoted; 5) the content of acetic acid, propionic acid and other short-chain fatty acids in the intestinal tract can be increased, and the intestinal tract health is promoted; and 6) the abundance of escherichia coli shigella, enterococcus and other pathogenic bacteria can be remarkably reduced, the abundance of lactobacillus is increased, and the intestinal flora is adjusted.

Description

technical field [0001] The invention belongs to the technical field of probiotics in poultry feed, and specifically discloses a goose-sourced plant lactobacillus and an application thereof. Background technique [0002] In recent years, goose meat food has continued to be popular, and the large-scale breeding of meat goose has grown rapidly. The goose industry has become one of the important ways to promote the development of animal husbandry. However, after the implementation of policies such as "banning antibiotics and water breeding", intestinal diseases of meat geese increased and immunity decreased. The use of probiotics to regulate intestinal homeostasis, improve the barrier protection function of intestinal mucosal microorganisms, and enhance the host's intestinal health and resistance to stress are one of the hotspots of intestinal health research at home and abroad. [0003] Intestinal microbes will change with physiological factors such as differences in food type...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/75A23K10/18A23K10/12A61K35/747A61P1/00A61P31/04C12R1/25
CPCC12N1/20A23K50/75A23K10/18A23K10/12A61K35/747A61P1/00A61P31/04A23V2400/169
Inventor 闫俊书宦海琳奚雨萌周维仁李悦徐辉
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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