Goose source lactobacillus plantarum and application thereof
A Lactobacillus plantarum, goose-derived technology, applied in the field of probiotics, can solve the problem of unresearched intestinal structure, intestinal metabolites of intestinal flora, no mention of avian intestinal cell adhesion, no mention of animal intestinal tract cell adhesion and other problems, to achieve the effect of fast acid production and strong lactic acid production ability
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Embodiment 1
[0028] Example 1 Isolation and Identification of Plant Lactobacillus (Lactobacillus plantarum) ER41
[0029] 1. Separation and purification
[0030] Isolate and purify Lactobacillus plantarum ER41 from goose intestinal tract, as follows: Weigh 1 g of intestinal chyme sample and add it to 99 ml of sterilized distilled water, shake it for 1 h on a constant temperature shaker, then dilute it 10 times in a gradient, and take 10 -3 、10 -4 The sample dilution solution was spread on the MRS solid medium, cultured for 48h, and then taken out, according to the size, shape and color of the colony, a single colony growing on the MRS was picked, and the catalase test and Gram staining were carried out . Catalase-negative and Gram-positive were tentatively designated as Lactobacillus, and streaked and purified on MRS solid medium for 2 times. Stored at -80°C in MRS medium with 25% glycerol, the strain was named ER41.
[0031] 2. Physiological and biochemical detection
[0032] Lactoba...
Embodiment 2
[0036] Example 2 Detection of Lactobacillus plantarum ER41 (Lactobacillus plantarum ER41) Acid Production Ability
[0037]Strain activation: inoculate the cryopreserved Lactobacillus plantarum ER41 in MRS liquid medium, culture at 37°C for 24 hours, and subculture twice in this way to obtain the activated Lactobacillus plantarum ER41; the activated strain is inoculated at a volume ratio of 3% The amount was re-transferred to MRS liquid medium, cultivated for 24 hours, and samples were taken at 4h, 8h, 12h, and 24h to measure the pH value and lactic acid production of the supernatant. The results are shown in Table 1. It can be seen from Table 1 that ER41 can produce acid rapidly, the pH value drops below 4.0 in 8 hours, and the lactic acid production in 24 hours is 165.87mmol / L, and the acid production is fast and the lactic acid production ability is strong.
[0038] Table 1: Acid production capacity of Lactobacillus plantarum ER41
[0039] time pH Lactic acid ...
Embodiment 3
[0040] Embodiment 3: the detection of antibacterial activity of Lactobacillus plantarum ER41 (Lactobacillus plantarum ER41)
[0041] Strain activation: inoculate the cryopreserved Lactobacillus plantarum ER41 in MRS liquid medium, culture at 37°C for 24 hours, and subculture twice in this way to obtain the activated Lactobacillus plantarum ER41; the activated strain is inoculated at a volume ratio of 3% The amount was retransferred to MRS liquid medium, cultivated for 24 hours, and the supernatant was obtained by centrifuging the bacterial solution.
[0042] Bacteria (Escherichia coli, Staphylococcus aureus, Salmonella, Listeria) were used as indicator bacteria, and LB medium was used as the culture medium for indicator bacteria. Activate the indicator strain preserved in glycerol. The activated strains were re-transferred into LA broth with 3% inoculum amount, and the indicator bacteria were cultured at 37°C for 24 hours, and the indicator bacteria solution was diluted with ...
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