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Additive premixing feed and application thereof

A premixed feed and additive technology, applied in the field of additive premixed feed, additive premixed feed and its preparation, can solve the problems of reduced disease resistance of farmed animals, enhanced drug resistance of pathogenic bacteria, and restrictions on the development of aquaculture. Achieve the effects of promoting animal production performance, preventing infection, prolonging storage time and quality

Pending Publication Date: 2020-05-19
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the additives currently used are chemically synthesized drugs, antibiotics and hormones. Excessive use has resulted in reduced disease resistance of farmed animals, enhanced drug resistance of pathogenic bacteria, and drug residues in products, which have seriously restricted the development of the aquaculture industry. develop

Method used

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  • Additive premixing feed and application thereof
  • Additive premixing feed and application thereof
  • Additive premixing feed and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of Wormwood Fermentation Broth

[0026] Streak the Lactobacillus plantarum WLPL01 cells on the MRS agar plate, culture anaerobically at 37°C for 24-48h, pick a single colony and inoculate it in MRS liquid anaerobic medium, and culture anaerobically at 37°C for 16h. Wash the cells twice with 1×PBS, and adjust the cell concentration to 10 7 cfu / mL, spare. Use a pulverizer to fully pulverize the wormwood, pass through an 80-mesh sieve, remove the wormwood, and get the wormwood powder. After mixing wormwood powder (10%) and glucose (5%) evenly, add distilled water of corresponding volume, drop into 10 7 cfu / mL Lactobacillus plantarum WLPL01, fermented at 37°C for 7 days to obtain a fermented product.

Embodiment 2

[0028] Antibacterial activity analysis of wormwood fermented liquor

[0029] E.coli O157:H7 CMCC 44828, C.albicans ATCC 14053, L. monocytogenes CMCC 54007, S.aureus CMCC 26003 and S.Typhimurium ATCC 13311 were used as indicator pathogens to evaluate the antibacterial properties of fermentation products.

[0030] As in Example 1, the Wormwood wormwood fermentation liquid was prepared, and the Wormwood wormwood fermentation liquid of the 0, 1, 3 and 5 days of fermentation was selected as the experimental sample. Use 80% (v / v) ethanol to extract L. plantarum WLPL01 wormwood fermentation liquid and natural wormwood fermentation liquid respectively, after ultrasonic treatment at 40°C for 1 hour, evaporate and concentrate, and freeze-dry to solid. Dissolve 1 mg of sample in 1 mL of double-distilled water and add 200 μL to an Oxford cup, and use 1×PBS buffer as a negative control. After the culture dish was placed in a 37°C incubator for 12 hours of aerobic culture, the size of the ...

Embodiment 3

[0034] Intervention effect of wormwood fermented liquid on mice infected with Salmonella typhimurium

[0035] In order to study the effect of wormwood fermented liquid as an additive premixed feed, 56 BALB / c female mice of 6-7 weeks were selected and raised in a mouse cage environment of 20°C-25°C, 12h light and dark cycle, natural drinking water and food standards rat food. After adapting to the environment for 7 days, they were randomly divided into 7 groups with 8 rats in each group. Including: blank group (C), Salmonella typhimurium infection model group (ST), 50mg / mL wormwood fermentation liquid group (T), high dose (50mg / mL) wormwood fermentation liquid group (H), medium dose (5mg / mL) group (M), low dose (0.5mg / mL) group (L) and Lactobacillus plantarum WLPL01 group (01).

[0036] From the first day of the experiment, the blank group C and the model group ST were continuously orally administered 200 μL of normal saline every day; the 50 mg / mL group T, the intervention ...

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Abstract

The invention relates to additive premixing feed, and belongs to the field of agricultural microbial feed additives. The feed additive contains lactobacillus plantarum WLPL01 and a culture obtained after Artemisia argyi Levl. et Van is fermented by the lactobacillus plantarum WLPL01. The lactobacillus plantarum WLPL01 fermentation culture has an antibacterial function for escherichia coli O157: H7, Listeria monocytogenes, staphylococcus aureus, Monilia albican and salmonella typhimurium. On a premise that the feed additive is not harmless to animals, the quality of the feed can be improved, afeed use ratio is improved, animal immunity can be effectively enhanced, the infection of the salmonella typhimurium is prevented, and the additive premixing feed has an important meaning for accelerating the sustainable development of breeding industry and guaranteeing product quality safety.

Description

technical field [0001] The invention relates to an additive premixed feed, belonging to the field of agricultural microbial feed additives. Specifically, it relates to an additive premixed feed fermented by Lactobacillus plantarum WLPL01 and a preparation method thereof. Background technique [0002] With the development of science and technology, the breeding industry is increasingly moving towards intensification and large-scale development, but a series of problems have also arisen during its development, such as the outbreak and spread of poultry salmonella infection, which has caused farmers to suffer huge losses. emergency loss. The application of feed additives helps farmers to solve this problem quickly and efficiently and improve the economic effect. Feed additives are a kind of small or trace substances added to the feed in order to meet the needs of raising animals; they can improve the feed conversion rate, improve the palatability of the feed and the health st...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18A23K10/30A23K50/50
CPCA23K10/12A23K10/18A23K10/30A23K50/50A23V2400/169
Inventor 陶雪莹魏华詹晖田林林杨琴何瑶裘梁
Owner NANCHANG UNIV
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