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Preparation method and application of dogwood seed extract

A technology of dogwood seeds and extracts, which is applied in the field of preparation of dogwood seeds extracts, can solve problems such as high viscosity of extracts, easy mildew of extracts, difficulty in filtration and concentration, etc., achieve good antibacterial activity, increase utilization value, and broaden utilization range effect

Inactive Publication Date: 2020-04-17
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These strict operating conditions do not utilize the promotion and application of this separation technology; Chinese invention patent (publication number 103110685A) discloses a water extract of Cornus officinalis fruit core, and the water extract is used in the preparation of diastolic thoracic aortic ring composition and blood pressure lowering In the application of the composition, the solvent used in this method is water, which can effectively reduce the cost of extraction, but after using water as the extract, the viscosity of the extract is high, it is difficult to filter and concentrate, and the extract is prone to mildew and affects subsequent tests

Method used

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  • Preparation method and application of dogwood seed extract
  • Preparation method and application of dogwood seed extract
  • Preparation method and application of dogwood seed extract

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The preparation of embodiment 1 Cornus officinalis seed extract

[0022] A preparation method of cornus officinalis extract, comprising the following steps:

[0023] a: Grinding the dry dogwood seed material into dogwood seed superfine powder (80 mesh), wherein the water content of the dogwood seed dry material is less than 13%;

[0024] b: add absolute ethanol to the superfine powder of Cornus officinalis seeds, the ratio of solid to liquid is 1:20, and soak for 24 hours under the condition of 50°C;

[0025] c: Take the soaking liquid obtained in step b and put it in a rotary evaporator, distill it under reduced pressure at 0.09kPa, freeze-dry it under low-temperature vacuum to obtain the crude extract of Cornus officinalis seeds.

Embodiment 2

[0026] Example 2 Cornus officinalis seed crude extract is to the mensuration of standard strain minimum inhibitory concentration

[0027] The test strain was expanded and cultivated overnight in TSB liquid medium (purchased from Solarbio Company, namely Beijing Soleibao Technology Co., Ltd.), and cultured at OD 600 When the OD value of the cultured bacteria liquid reached 0.6-0.8, the cultured bacteria liquid was centrifuged at 6000r / min for 10min, the supernatant was discarded after centrifugation, the bacterial precipitate was collected, and then an equal volume of Resuspend the bacteria in 0.01MPBS; then dilute the bacteria solution to be tested to 2×10 with MH medium 6 CFU / ml. Dilute the cornus officinale seed extract solution with deionized water to a concentration of 10 μg / mL˜320 μg / mL. Add 50 μL of crude extract dilutions of Cornus officinalis seeds and 50 μL of diluted bacteria solution to each well of the cell culture plate, repeat the experiment for 3 wells at each...

Embodiment 3

[0032] Example 3 Detection of Hemolytic Activity of Cornus officinalis Crude Extract

[0033] Take 50mL of fresh rabbit blood, centrifuge at 3000rpm for 20min, discard the supernatant, wash the erythrocytes with isotonic PBS buffer, centrifuge repeatedly three times until the supernatant is colorless and transparent, and prepare rabbit erythrocyte suspension; take 1.5mL centrifuge tube 5 No. 1 to 5; 2 mg, 1 mg, and 0.5 mg of the dry powder of cornus officinalis seed crude extract of the present invention were added to tubes 1 to 3, respectively, and 800 μL of 0.01M PBS (pH 7.2) buffer solution was added to each tube. Add 800 μL of PBS buffer solution to No. 4 tube, which is the negative control group; add 800 μL, 1% Triton X-100 deionized water dilution solution (which can completely hemolyze the red blood cells) to No. 5 tube, which is the positive control group. 1~5 Add 200μL red blood cell suspension to each tube. After standing still for 3 hours, tubes 1-5 were centrifuge...

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Abstract

The invention discloses a preparation method of a dogwood seed extract. The preparation method comprises the following steps: a, crushing dried dogwood seeds into ultrafine dogwood seed powder; b, adding absolute ethyl alcohol into the ultrafine dogwood seed powder, and carrying out soaking for 24 hours at a temperature of 50 DEG C; and c, putting a soaking solution into a rotary evaporator, carrying out reduced-pressure distillation, and performing drying to obtain a crude dogwood seed extract. The crude dogwood seed extract has a good antibacterial effect on escherichia coli and salmonella,and has no obvious cytotoxicity on rabbit red blood cells. According to the preparation method of the dogwood seed extract, only one-step extraction through ethyl alcohol is needed, extraction time isshort, extraction efficiency is high, the obtained effective components of dogwood seeds are high in amount, and the influence of experimental conditions on extraction is small.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a preparation method and application of cornel seed extract. Background technique [0002] Cornus officinalis, also known as medicinal jujube or Shuzao, has the functions of enhancing the body's immune function, anti-cancer, diuretic, lowering blood pressure and anti-oxidation. Cornus officinalis dried and ripe pulp is a nourishing and precious medicinal material. Studies have found that the cornel pulp contains active ingredients such as cornus polysaccharides, organic acids, and tannins. Among them, cornus polysaccharides especially have immune regulation, anti-aging and other effects. Dogwood pulp is also widely used in the food industry. And dogwood seeds are often discarded as a by-product of processing. [0003] Studies have found that the extract of Cornus officinalis seeds has the effect of relaxing arteries and lowering blood pressure. For example, the Chinese...

Claims

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Application Information

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IPC IPC(8): A61K36/40A61P31/04A61K131/00
CPCA61K36/40A61K2236/15A61K2236/33A61K2236/51A61P31/04Y02A50/30
Inventor 徐彦召许明录王青胡建和王芳王玲彩赵雪芹张慧辉白跃宇
Owner HENAN INST OF SCI & TECH
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