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Bifidobacterium lactis strain J605 and application thereof

A technology of bifidobacterium lactis and strains, applied in the field of microorganisms, can solve sensitive problems, achieve the effect of improving intestinal environment, high resistance to gastrointestinal stress, and good intestinal probiotics

Active Publication Date: 2021-07-09
湖北均瑶大健康饮品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bifidobacterium lactis, which has inhibitory effects on various pathogenic bacteria, is sensitive to various antibiotics and regulates various probiotics, has not been reported in this field

Method used

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  • Bifidobacterium lactis strain J605 and application thereof
  • Bifidobacterium lactis strain J605 and application thereof
  • Bifidobacterium lactis strain J605 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 4

[0043] Escherichia coli, Staphylococcus aureus, and Salmonella typhi used in Experimental Example 4 are all commercially available;

experiment example 8

[0044] The BALB / c male mice used in Experimental Example 8 are commercially available.

[0045] The first group of embodiments, Bifidobacterium lactis bacterial strain J605 of the present invention

[0046] The embodiment of this group provides a Bifidobacterium lactis strain J605, the preservation number of which is CGMCC No.21751.

[0047]Based on the bacterial strain J605 provided by this group of examples and the content recorded in the description of the present invention, according to the enlightenment of the present invention, those skilled in the art can use the bacterial strain J605 for various industrial production or medical care purposes, for example, the bacterial strain J605 can be used for Food or medicine can be prepared, and the bacterial strain J605 can also be directly used for treatment or health care of humans or animals. Any act of using, producing, selling, or offering to sell the bacterial strain J605 falls within the protection scope of the present in...

experiment example 1

[0073] Experimental example 1, the separation and screening of bacterial strain J605 of the present invention

[0074] (1) Sample collection

[0075] Stool samples from healthy infants aged 0 to 1, who had not taken antibiotics within three months and were exclusively breastfed.

[0076] (2) Strain isolation

[0077] Take 1g sample and put it into 9mL normal saline for shaking and mixing. After the bacterial suspension is gradiently diluted, draw 100μL of the appropriate gradient bacterial solution on the bifidobacterium isolation medium agar plate added with mupirocin lithium salt, and spread evenly , after replacing the air with anaerobic air, the cells were cultured at a constant temperature of 37°C for 48h. After the colonies grow out, pick opaque milky white, round and shiny colonies with neat edges, raised and wet surfaces, and perform repeated streaking and purification on MRS solid medium to obtain a bacterial strain of infant feces, and then in the Store at 4°C unt...

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PUM

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Abstract

The invention relates to a Bifidobacterium lactis strain J605 and an application thereof, and belongs to the technical field of microorganisms. The preservation number of the Bifidobacterium lactis strain J605 is CGMCC (China General Microbiological Culture Collection Center) No.21751. The strain J605 has a good intestinal tract probiotic function and relatively high gastrointestinal tract stress resistance, has an obvious inhibition effect on escherichia coli, staphylococcus aureus and salmonella, has sensitivity to common antibiotics, and can be used for preparing a feed additive for preventing and treating intestinal diseases. The compound can be widely applied to medicines, health-care foods or drinks for improving the intestinal environment, and has high market application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bifidobacterium lactis strain J605 and an application thereof. Background technique [0002] With the acceleration of the social rhythm and the improvement of living standards, great changes have taken place in people's diet structure, and the incidence of constipation is also increasing. Chronic constipation is a common symptom of gastrointestinal disorders worldwide, and it may also cause other diseases. At the same time, the increase in the incidence of chronic constipation seriously affects people's quality of life. There are many factors that affect chronic constipation, such as age, eating habits, lifestyle, etc., among which age is a major factor affecting constipation. With the increase of age, the number of beneficial bacteria in the intestinal tract gradually decreases, coupled with the degeneration of various functional organs of the body, the pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L33/14A23L2/38A23L2/52A61K35/745A61K35/747A61P1/12A61P1/10A61P1/00A61P1/14A61P31/04C12R1/01A61K35/744A61K35/74
CPCC12N1/20A23L33/135A23L33/14A23L2/382A23L2/52A61K35/745A61K35/744A61K35/747A61K35/742A61P1/12A61P1/10A61P1/00A61P1/14A61P31/04A23V2002/00A23V2400/531A23V2200/3204A61K2300/00Y02A50/30
Inventor 王均豪朱航明叶亚明
Owner 湖北均瑶大健康饮品股份有限公司
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