6 results about "Acid tolerant" patented technology

The invention belongs to the technical field of biological pharmacy, and relates to a method for preparing tremella aurantialba polyphenol by utilizing tremella aurantialba TA58.21 fungus chassis cell fermentation, which comprises the following steps: adding a cooked soybean meal culture medium and a tremella aurantialba seed solution into a fermentation tank, introducing sterile oxygen, controlling the stirring rotating speed of the fermentation tank and the culture temperature to be 25-30 DEG C, continuously culturing for 5-8 days, and when the fermentation liquor becomes red, stopping the fermentation; when a large amount of foam is generated, closing an exhaust valve, keeping the tank pressure at 0.1 MPa, increasing the fermentation temperature to 50 DEG C, continuing to culture for 24 hours, increasing the stirring rotating speed of the fermentation tank, heating the fermentation liquor to 80-90 DEG C, keeping the temperature for 4-8 hours, stopping stirring, continuing to heat to 120 DEG C, and keeping the temperature for 30 minutes; centrifuging the fermentation liquor to remove precipitates and mycelia; collecting supernate, and pre-treating macromolecular substances and thallus fragments; collecting permeate liquid, and intercepting through a nanofiltration membrane to obtain a concentrated solution; and carrying out spray drying or freeze drying treatment on the concentrated solution to obtain a high-purity product. According to the invention, the tremella aurantialba polyphenol product with good water solubility, heat resistance, acid resistance and molecular weight of less than 10000 Da can be obtained.

This application discloses an acid-tolerant Cyberlindnera jadinii yeast strain and its applications, belonging to the field of microbial technology. The Cyberlindnera jadinii yeast strain is Cyberlindnera jadinii C-12-2, which was deposited on Mar. 5, 2024, at the Guangdong Microbial Culture Collection Center, with the deposit number GDMCC NO: 64388. The Cyberlindnera jadinii yeast disclosed in this application can grow in a medium with a pH of 3.5, and its crude protein content can reach 70.02% after 24 hours of fermentation. The yeast strain disclosed in this application was obtained through heavy-ion irradiation mutagenesis followed by acid adaptation. It has been determined that the strain can grow in an acidic medium with a pH of 3.5, overcoming the growth limitation of the original strain at low pH values.

The invention relates to the technical field of underground water pollution low-carbon treatment, in particular to a method for synchronously removing benzene series and hexavalent chromium in acidic underground water, which comprises the following steps: gradually replacing electron acceptors required by microorganisms, obtaining acid-resistant anaerobic mixed flora capable of degrading the benzene series and simultaneously generating iron minerals from soil, the method comprises the following steps: adding ferric citrate and an acid-resistant anaerobic mixed flora into acidic polluted underground water containing benzene series and hexavalent chromium, culturing, degrading the benzene series, reducing the hexavalent chromium, and fixing part of the hexavalent chromium in iron minerals. According to the method, anaerobic microorganisms are used for degrading benzene series and generating iron minerals under the acidic condition, and in the process, hexavalent chromium is reduced and fixed by the iron minerals at the same time. Compared with the prior art, the method is suitable for acidic polluted underground water, does not need additional pH regulation and control, does not need oxygen supply, is simple to operate, can synchronously repair combined pollution, and is environment-friendly and free of secondary pollution.

The present invention belongs to the technical field of feed additives, and specifically provides a composition for improving the stress resistance of Enterococcus faecium. By mass percentage, it includes: 1% succinic acid, 0.05% sodium citrate, 0 - 1.2% thickener, 12% carrier, and the balance is water. All raw materials used in the composition for improving the stress resistance of Enterococcus faecium provided by the present invention are raw materials permitted to be added in the feed additive industry, ensuring the compliance and safety of the product. The present invention also provides a method for post-treating Enterococcus faecium with this composition to prepare heat-resistant and acid-resistant Enterococcus faecium. This method is simple and easy to implement, and through the synergistic effect of each component and the control of pH during the preparation process, the heat-resistant retention rate of the obtained Enterococcus faecium preparation at 75°C can be increased to 53.8%, and at the same time, the gastric acid tolerance at pH 2.5 can be increased to 60.5%, significantly improving the stress resistance of Enterococcus faecium and enabling it to better adapt to industrial production applications.

Described herein are methods, microbial cell lines, and media used in co-culture to augment propionic acid production using an optimized fermentation medium and methods for increasing propionic acid yield, e.g., by co-culturing Lacticbacillus Casei and high-acid tolerant A. Acidipropionici.

The invention belongs to the technical field of microorganisms, and provides a Sarocladium sp. FT1 mycelium pellet with functions of synchronously removing nitrogen and carbon from acidic high-concentration organic wastewater and application of the Sarocladium sp. FT1 mycelium pellet. The strain broom cladosporium sp. FT1 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 11, 2024, and the preservation number is CGMCC NO.41716. The invention further discloses a preparation method of the bacterial strain. The strain is an acid-resistant heterotrophic nitrification and aerobic denitrification fungus, and can effectively remove inorganic nitrogen (ammonia nitrogen and nitrate nitrogen) in acidic wastewater while utilizing an organic carbon source under the conditions that C / N is 10-40 and pH is 3-7. The strain can form mycelium pellets in a special growth state, not only can synchronously remove carbon and nitrogen, but also has very good settling performance, so that the strain can play a role in the fields of low-pH and high-organic-load sewage nitrogen and carbon removal treatment, water body remediation and the like.