Bacillus subtilis strain and method for preparing Pu'er tea capable of regulating intestinal functions from same
A technology of Bacillus subtilis and Pu-erh tea, applied in the field of microorganisms and food biology, can solve problems such as increasing the chance of food-borne pathogenic bacteria contamination, and achieve the effects of assisting in regulating intestinal function, promoting growth and genetic stability.
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[0041] Embodiment 1 patent Pu'er tea
[0042] According to the method of the present invention, a single-strain fermentation process is adopted to prepare Pu'er ripe tea by using Bacillus subtilis (CGMCC No.15959). Proceed as follows:
[0043] 1. After blending and mixing different raw materials according to the requirements, it is required that the tea leaves have no flowers after mixing;
[0044] 2. Inoculate the prepared liquid seeds into the tea raw materials, the inoculum amount is 28%, the bacteria liquid is required to be sprayed evenly, the tea leaves are not agglomerated, and the density of the liquid seeds is 5*10 7 cfu / mL
[0045] 3. Stack the inoculated tea leaves into a small tea pile with a height of 0.5 meters and a length and width of 1*1 meter, and carry out pile fermentation;
[0046] 4. Turn the pile once every 5 days to allow the core tea leaves to mix evenly with the opposite pile surface tea leaves, and maintain the core temperature and moisture conten...
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