A kit for simultaneously detecting antibodies against avian leukosis virus and Salmonella pullorum

Abstract

The invention relates to the technical field of animal epidemic disease detection and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. The kit comprises: P27 protein, GroEL-delta8-1 protein, an enzyme labeled second antibody, BSA (Bovine Serum Albumin), coating liquid, confining liquid, a color developing solution anda stopping solution. The invention develops an indirect ELISA (Enzyme Linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorumantibody by taking prokaryotic expression protein of a conserved region P27 of an avian leukosis virus and prokaryotic expression protein of truncated protamine GroEL-delta8-1 of a dominant antigen GroEL of salmonella pullorum as coating antigens; the kit provided by the invention can be used for simultaneously detecting avian leucosis and pullorosis; chickens which are detected to be positive areeliminated so that the aim of simultaneously purifying is realized and the clinical detection and purification work is extremely alleviated.

Description

technical field [0001] The invention relates to the technical field of animal disease detection, in particular to a kit for simultaneously detecting antibodies to avian leukosis virus and Salmonella pullorum. Background technique [0002] Avian Leukosis is a general term for a variety of tumor diseases in poultry caused by viruses in the Avian Leukosis Virus (ALV) and Avian Sarcoma Virus (ASV) groups. The disease can cause many infectious benign and malignant tumors in chickens. Since Roloff reported chicken lymphosarcoma in 1868, avian leukemia has been distributed all over the world, and natural cases have also occurred in my country. The disease can cause slow weight gain, delayed sexual maturity, small eggs, thin eggshells, decreased egg production, low fertilization rate and hatchability, increased carcass waste rate, and increased mortality, causing direct economic losses. At the same time, it can also cause the body's non-specific resistance to decrease and immunosu...

AI Technical Summary

Problems solved by technology

[0008] Both avian leukemia and pullorum are infectious diseases that need to be purified in chicken farms. At present, the purification of avian leukemia is mainly to use foreign ELISA kits to detect the avian leukemia virus antigen p27 in the chicken cloacal swab and the avian leukemia antibody in the chicken serum. The purification of pullorum mainly uses plate agglutination test to detect the antibody of Salmonella pullorum in chicken serum. These kits or methods are all aimed at the detection of a single infectious disease. There is no single kit that can detect the two diseases of avian leukemia and pullorum

The kit for detecting avian leukosis alone and the method of plate agglutination test for detecting pullorum alone make the clinical detection and purification of pullorum and pullorum consume a lot of manpower, material resources and financial resources

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