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Method for detecting ochratoxin A based on near-infrared up-conversion luminescence marking and magnetic separation

A technology of ochratoxin and luminescent labeling, which is applied in the field of nanomaterials and analytical chemistry, can solve the problems that antibodies are easily affected by external conditions, a large amount of manpower and financial resources, tedious and time-consuming, etc., to facilitate chemical modification, improve stability and accuracy performance, sensitivity enhancement effect

Inactive Publication Date: 2016-10-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antibodies are easily affected by external conditions, especially temperature, and the preparation of antibodies requires animal or cell experiments, which is tedious, time-consuming, and costly; while instrument-based analysis methods require a lot of manpower and financial resources, and are often only used for experiments chamber analysis

Method used

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  • Method for detecting ochratoxin A based on near-infrared up-conversion luminescence marking and magnetic separation
  • Method for detecting ochratoxin A based on near-infrared up-conversion luminescence marking and magnetic separation
  • Method for detecting ochratoxin A based on near-infrared up-conversion luminescence marking and magnetic separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Establishment of standard curve for detection of ochratoxin A in actual beer samples and pretreatment of test samples: beer samples were refrigerated at 4° C. for 30 minutes, and degassed by ultrasound. Take 20g of degassed beer sample, put it in a 25mL volumetric flask, add 2% sodium bicarbonate and 15% sodium chloride and mix the extract to the mark, mix well, filter with glass fiber filter paper until clear, collect the filtrate for later use.

[0032] 5 different types of beer were purchased from a local supermarket, and the content of ochratoxin A was determined by using the method of the present invention and the enzyme-linked immunosorbent method, and the results are shown in Table 1. The detection results of the two methods were consistent with no significant difference.

[0033] Table 1: The actual sample detection of beer, the method of the present invention is compared with the ELISA method

[0034]

[0035] Note: ND is not detected

Embodiment 2

[0036] Example 2: The detection of ochratoxin A in the actual beer sample and the recovery rate of standard addition The experimental sample pretreatment is the same as that in Example 1.

[0037] Using the 5 groups of ochratoxin A concentration data obtained in Example 1 as the background value, five OTA standard substances with different concentrations were added thereto, and the OTA content was detected again by the method of the present invention to obtain the detected value. Recovery %=(detection value-background value) / addition amount×100%. From the data in Table 2, it can be seen that the recovery rate is 90.0%-105.0%, indicating that the present invention is stable, sensitive and accurate, and is applicable to the detection of OTA in actual beer samples.

[0038] Table 2: Detection and recovery of ochratoxin A in actual beer samples

[0039]

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Abstract

A method for detecting ochratoxin A based on near-infrared up-conversion luminescence marking and magnetic separation is used for detecting ochratoxin A (OTA) content in wheat and its products and the like. By connecting near-infrared up-conversion luminescent material with NaYF4:Yb 0.2 and Tm 0.02 with ochratoxin A aptamer to form a signal probe, the signal probe and aptamer complementary oligonucleotide single strand modified Fe3O4 magnetic nanomaterial form a nano composite, and at the moment up-conversion luminescence signal is maximum; when OTA is present in a detection system, OTA specifically binds with OTA aptamer so that double strands are unlinked, and by monitoring up-conversion luminescence signal strength 804 nm from a near-infrared zone, it is possible to quantitatively detect OTA, with a linear range of 0.01-100 ng / mL and a detection limit of 0.005 ng / mL. The method for OTA detection has the advantages of high sensitivity, high speed and good simplicity and is applied to detecting beer samples, with accurate and reliable results.

Description

technical field [0001] A detection method for ochratoxin A based on near-infrared up-conversion luminescence labeling and magnetic separation, relates to the technical field of nanomaterials and analytical chemistry, and is used for detecting ochratoxin A in food. Background technique [0002] Ochratoxin A (Ochratoxins A, OTA) is mainly produced by three fungi, Penicillium Verrucosum, Aspergillus achraceus and A. carbonarius, and many other toxin-producing strains are also continuously was reported. OTA mainly pollutes plant products, such as grains, beans, peanuts, raisins, coffee, beer and red wine. Many reports show that OTA also exists in some animal products. of plant-based products lead to OTA enrichment in animals. OTA has strong liver, kidney and neurotoxicity and teratogenic, carcinogenic and mutagenic effects, which cause great harm to human health and food safety. Therefore, the establishment of accurate, sensitive and rapid OTA detection technology is of great...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/543G01N33/54393G01N2333/37
Inventor 王周平戴邵亮吴世嘉段诺
Owner JIANGNAN UNIV
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