Combined live vaccine against porcine reproductive and respiratory syndrome and pseudorabies, and preparation method thereof

A technology for respiratory syndrome and porcine pseudorabies, applied in antiviral agents, pharmaceutical formulations, viral antigen components, etc., can solve problems such as difficult to achieve effects

Active Publication Date: 2012-10-17
华威特(江苏)生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, even if porcine reproductive and respiratory syndrome vaccine is immunized with

Method used

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  • Combined live vaccine against porcine reproductive and respiratory syndrome and pseudorabies, and preparation method thereof
  • Combined live vaccine against porcine reproductive and respiratory syndrome and pseudorabies, and preparation method thereof
  • Combined live vaccine against porcine reproductive and respiratory syndrome and pseudorabies, and preparation method thereof

Examples

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[0080] Compared with the prior art, the preparation method provided by the invention has many advantages. For example, its preparation process is simple and stable, easy to operate, high virus content, small batch-to-batch differences, easy to control quality, can significantly improve vaccine yield and quality, and reduce allergic reactions. The porcine reproductive and respiratory syndrome and pseudorabies combined live vaccine obtained by using the preparation method of the present invention has good safety and high immune efficacy, and has a good immune protection effect against the virulent attack of porcine reproductive and respiratory syndrome and pseudorabies.

[0081] The invention also provides a PRRSV and PRV dual vaccine composition prepared by the method of the invention.

[0082] The invention also provides the use of the vaccine composition in preparing biological products for preventing or treating porcine reproductive and respiratory syndrome and porcine pseudorabi...

Example Embodiment

[0091] Example 1 Preparation of highly pathogenic porcine reproductive and respiratory syndrome and pseudorabies combined live vaccine

[0092] The highly pathogenic porcine reproductive and respiratory syndrome and pseudorabies combined live vaccine is prepared by the following method:

[0093] (1) Select susceptible cells as cells for seedling production: select African green monkey kidney cells (Marc-145) as PRRSV susceptible cells, and select Marc-145, bovine kidney cells (MDBK) and bovine testis passage cells (BT) Passage cells are susceptible to PRV.

[0094] (2) Passage and culture of cells for seedling production: the above-mentioned passaged cells are digested and passaged with EDTA-trypsin cell dispersion, and then cultured with cell growth fluid to form a monolayer of passaged cells, used for continued passage or virus inoculation; cell culture The temperature is 36-37°C.

[0095] (3) Propagation of virus seeds for production:

[0096] A. Propagation of virus seeds for the ...

Example Embodiment

[0106] Example 2 Purity test of highly pathogenic PRRSV and PRV dual live vaccine

[0107] According to the description on pages 15, 19, and 20 of Appendix of the "Chinese Ming Republic Veterinary Pharmacopoeia" (2005 edition), the dual live vaccine prepared in Example 1 was tested, and the results showed that the dual live vaccine was free of bacteria, molds and mycoplasma Growth, no foreign virus contamination.

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Abstract

The invention provides a combined live vaccine for preventing porcine reproductive and respiratory syndrome and pseudorabies, and a preparation method and application thereof. According to the invention, no immunosuppression occurs between two vaccines of the combined live vaccine; compared with each single vaccine, the combined live vaccine has no obvious difference in security, immunogenicity, immunity duration and immuno-protective effects and has remarkable immuno-protective effects on preventing porcine reproductive and respiratory syndrome and pseudorabies.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a dual vaccine of porcine reproductive and respiratory syndrome and porcine pseudorabies and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcine Reproductive and Respiratory Syndrome, PRRS) and pseudorabies (Pseudorabies, PR) are two important infectious diseases that endanger the intensive pig industry. Huge economic loss. Immunization is an important measure for the prevention and control of porcine reproductive and respiratory syndrome and pseudorabies. [0003] However, there are many types of vaccines for pigs. In actual operation, it is difficult to rationally arrange multiple injections, multiple doses, and various immunization procedures. It is cumbersome, time-consuming, laborious, and increases costs. Frequent immunization can also easily cause immune paralysis and in...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K48/00A61P31/14A61P31/22A61K39/12A61K39/245
Inventor 武华
Owner 华威特(江苏)生物制药有限公司
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