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Combined live vaccine against porcine reproductive and respiratory syndrome and pseudorabies, and preparation method thereof

A technology for respiratory syndrome and porcine pseudorabies, applied in antiviral agents, pharmaceutical formulations, viral antigen components, etc., can solve problems such as difficult to achieve effects

Active Publication Date: 2012-10-17
华威特(江苏)生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, even if porcine reproductive and respiratory syndrome vaccine is immunized with other vaccines, it is difficult to achieve the same effect as single vaccine immunization

Method used

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  • Combined live vaccine against porcine reproductive and respiratory syndrome and pseudorabies, and preparation method thereof
  • Combined live vaccine against porcine reproductive and respiratory syndrome and pseudorabies, and preparation method thereof
  • Combined live vaccine against porcine reproductive and respiratory syndrome and pseudorabies, and preparation method thereof

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Experimental program
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Effect test

preparation example Construction

[0080] Compared with the prior art, the preparation method provided by the invention has many advantages. For example, its preparation process is simple and stable, easy to operate, high virus content, small batch-to-batch variation, easy quality control, can significantly improve vaccine yield and quality, and reduce allergic reactions, etc. The porcine reproductive and respiratory syndrome and pseudorabies dual live vaccine obtained by the preparation method of the present invention has good safety and high immune efficacy, and has better immune protection against the virulent attack of porcine reproductive and respiratory syndrome and pseudorabies.

[0081] The invention also provides the PRRSV and PRV dual vaccine composition prepared by the method of the invention.

[0082] The invention also provides the use of the vaccine composition in the preparation of biological products for preventing or treating porcine reproductive and respiratory syndrome and porcine pseudorabie...

Embodiment 1

[0091] The preparation of embodiment 1 highly pathogenic porcine reproductive and respiratory syndrome, pseudorabies dual live vaccine

[0092] Prepare highly pathogenic porcine reproductive and respiratory syndrome and pseudorabies dual live vaccine by the following method:

[0093] (1) Select susceptible cells as cells for seedling preparation: African green monkey kidney cells (Marc-145) were selected as susceptible cells of PRRSV, and Marc-145, bovine kidney cells (MDBK) and bovine testicular passage cells (BT) were selected. Passage cells are susceptible to PRV.

[0094] (2) Passage and culture of cells for seedling production: the above-mentioned passage cells are digested and passaged by EDTA-trypsin cell dispersion liquid, and continue to be cultured with cell growth medium. The temperature is 36-37°C.

[0095] (3) Propagation of poisonous species for production:

[0096] A. Propagation of virus seeds for production of highly pathogenic PRRSV live vaccine: In the ME...

Embodiment 2

[0106] The purity test of embodiment 2 highly pathogenic PRRSV, PRV dual live vaccine

[0107] According to the description on pages 15, 19, and 20 of the appendix of "The Veterinary Pharmacopoeia of the Republic of China" (2005 edition), the double live vaccine prepared in Example 1 is tested, and the result shows that the double live vaccine has no bacteria, mold and mycoplasma Growth without exogenous virus contamination.

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Abstract

The invention provides a combined live vaccine for preventing porcine reproductive and respiratory syndrome and pseudorabies, and a preparation method and application thereof. According to the invention, no immunosuppression occurs between two vaccines of the combined live vaccine; compared with each single vaccine, the combined live vaccine has no obvious difference in security, immunogenicity, immunity duration and immuno-protective effects and has remarkable immuno-protective effects on preventing porcine reproductive and respiratory syndrome and pseudorabies.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a dual vaccine of porcine reproductive and respiratory syndrome and porcine pseudorabies and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcine Reproductive and Respiratory Syndrome, PRRS) and pseudorabies (Pseudorabies, PR) are two important infectious diseases that endanger the intensive pig industry. Huge economic loss. Immunization is an important measure for the prevention and control of porcine reproductive and respiratory syndrome and pseudorabies. [0003] However, there are many types of vaccines for pigs. In actual operation, it is difficult to rationally arrange multiple injections, multiple doses, and various immunization procedures. It is cumbersome, time-consuming, laborious, and increases costs. Frequent immunization can also easily cause immune paralysis and in...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K48/00A61P31/14A61P31/22A61K39/12A61K39/245
Inventor 武华
Owner 华威特(江苏)生物制药有限公司
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