Method for producing pseudorabies living vaccines by using subculture cell source and product thereof

A technology for passaged cells and pseudorabies, which is applied in biochemical equipment and methods, medical preparations containing active ingredients, microorganisms, etc., can solve the difficulty of improving the yield and efficacy of vaccines, many cell debris and impurity proteins, and easily cause allergies. Response and other problems, to achieve the effect of good immune protection, high virus content, and improved vaccine yield and quality

Active Publication Date: 2010-04-21
广东永顺生物制药股份有限公司
View PDF0 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difference in raw materials of each batch of chicken embryos, the difference between batches of chicken embryo fibroblast primary cells is relatively large; the titer of toxin production is not high when chicken embryo fibroblasts are used to culture pseudorabies virus, which brings great benefits to the improvement of vaccine production and efficacy. Difficult, and there are many cell debris and miscellaneous proteins, which can easily cause allergic reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] In order to make the present invention easier to understand, the present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The specific experimental methods not mentioned in the following examples are generally carried out according to conventional experimental methods.

[0022] The production process method of producing pseudorabies live vaccine with passage cell (cell line), concrete steps are as follows:

[0023] (1) Select passage cells as cells for seedling preparation: in this embodiment, pig testis (ST) passage cells are selected, and in the other two examples, pig kidney (PK15) passage cells and pig kidney (IBRS- 2) Subculture cells.

[0024] (2) Passage and culture of cells for seedling preparation: the passaged cells were digested and passaged with EDTA-trypsin cell dispe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for producing pseudorabies living vaccines by using a subculture cell source and a pseudorabies living vaccine product thereof. The method comprises the following steps: culturing pseudorabies virus low-virulent strains by using subculture cells; harvesting the strains to obtain cell culture venom; and then adding a stabilizing agent and an antibiotic into the cellculture venom, and freezing and vacuum-drying the mixture to obtain the pseudorabies living vaccines of the subculture cell source. The subculture cells are subculture cells ST of pig testicle or subculture cells PK15 or IBRS-2 of pig kidney. The method for producing the pseudorabies living vaccines by using the subculture cell source has the advantages of simple and stable production process, easy operation, high virus content, little batch difference and controllable quality, can remarkably improve the yield and quality of the vaccines and reduce the anaphylactic reaction and the like. The pseudorabies living vaccines obtained by using the production method of the invention have good safety and high immune efficacy, and have better immune protection effect on pseudorabies virulent attack.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a method for producing a pseudorabies live vaccine from a subcultured cell source and a product thereof. Background technique [0002] Pseudorabies is an acute infectious disease of various animals caused by pseudorabies virus (PRV). The epidemic spreads throughout the world's major pig-growing countries. Immunization is the main strategy to control pseudorabies. [0003] The cells used in the production of live pseudorabies vaccines in my country are chicken embryo fibroblasts. Due to the difference in raw materials of each batch of chicken embryos, the difference between batches of chicken embryo fibroblast primary cells is relatively large; the titer of toxin production is not high when chicken embryo fibroblasts are used to culture pseudorabies virus, which brings great benefits to the improvement of vaccine production and efficacy. D...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245A61P31/20C12N7/00
Inventor 林旭野吴文福张毓金岑小清游启有任向阳廖润科周泽标张木辉司徒剑谋
Owner 广东永顺生物制药股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap