Quick measuring method of pyrethrin

A technology for rapid determination of pyrethrins, which is applied in the preparation of test samples and the measurement of color/spectral properties, etc., which can solve the problem of preferential utilization of high-content pyrethrum genotypes that affect the breeding process, distinguishing high-content pyrethrum from ordinary pyrethrum, and unsuitable for pyrethrum. The problem of rapid determination of esters, etc., to achieve the effect of fast test speed, simple determination method and low test cost

Inactive Publication Date: 2010-12-08
HUAZHONG AGRI UNIV
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Problems solved by technology

[0004] In terms of production, there are many reports on the processing and extraction technology of pyrethrins, mostly using supercritical carbon dioxide extraction (Kiriamiti, 2003), or adding ultrasonic or microwave-enhanced extraction process on the basis of organic solvent extraction (Zhang Yuchi et al., 2007; Chen Shouhong, 2003), but these methods only extract pyrethrins, cannot measure its content, and are only suitable for mass production in factory workshops
Under laboratory conditions, the American AOAC standard was the earliest reference standard for the detection of pyrethrins, but with the development of chromatographic technology, detection and analysis methods such as thin-layer chromatography, gas chromatography and high-performance liquid chromatography appeared (section Wei et al., 2007), but thin-layer chromatography is only suitable for rough quantification, and the accuracy is poor. The latter two methods not only have problems such as complicated procedures, long time consumption, cumbersome purification and pretreatment steps, and large solvent consumption. It needs to be carried out with the help of expensive instruments and equipment, which is obviously not suitable for the rapid determination of pyrethrin content in a large number of samples in the field
Therefore, whether the determination of pyrethrin content can be carried out quickly and effectively is a supporting measure that needs to be solved urgently, otherwise it will not be possible to quickly distinguish high-content pyrethrum from common pyrethrum, thereby affecting the breeding process and the priority utilization of high-content pyrethrum genotypes

Method used

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  • Quick measuring method of pyrethrin
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In a pyrethrum planting field, randomly select 20 pyrethrum individual plants according to the "S"-shaped route, and mark them. During the flowering stage of pyrethrum, when the tubular flowers are 50% to 99% open, pick 15 fresh flower heads of pyrethrum (No. 013), place them in an oven at 80°C for 36 hours, and weigh 100 mg after being pulverized by a pulverizer. Pyrethrum dried flower powder in a glass centrifuge tube, immediately add 5mL of n-hexane, tighten the screw cap of the centrifuge tube, vibrate on a vortex mixer for 20s, and then ultrasonic (40KHz, 220V; KQ-300DE CNC ultrasonic cleaner, Kunshan City, Jiangsu Province) (manufactured by Ultrasonic Instrument Co., Ltd.) in a water bath for 8 minutes, and after taking it out, continue to vibrate on a vortex mixer for 20 seconds, and then centrifuge at 1800 rpm at room temperature 20-25°C for 5 minutes to obtain a supernatant. Draw 0.4mL of the supernatant with a glass dropper, dilute the supernatant 40 times by ...

Embodiment 2

[0026] Sampling according to the method and steps of Example 1, the sample number is No. 002, 18 flowers are picked, placed in a 60°C oven for drying for 50h, after being pulverized by a pulverizer, weigh 50mg of pyrethrum dried flower powder in a glass centrifuge tube, and immediately Add 5mL of n-hexane, tighten the screw cap of the centrifuge tube, vibrate on the vortex mixer for 30s, then ultrasonic water bath for 12min, take it out and continue to vibrate on the vortex mixer for 30s, then centrifuge at 2500rpm, room temperature 20-25℃ for 7min , to obtain the supernatant. Draw 0.2mL of the supernatant with a glass dropper, dilute the supernatant 40 times with absolute ethanol by volume as the test solution, and dilute 40 times with an equal volume of n-hexane with absolute ethanol as the For the reference solution, the average absorbance value measured three times by a 752-type ultraviolet-visible spectrophotometer at a wavelength of 225 nm is 0.525, and the pyrethrin con...

Embodiment 3

[0028] Sampling according to the method and steps of Example 1, the sample number is No. 006, 20 flowers are picked, placed in an oven at 45°C for 40 hours, after being pulverized by a pulverizer, weigh 100 mg of pyrethrum dried flower powder in a glass centrifuge tube, and immediately Add 5mL of n-hexane, tighten the screw cap of the centrifuge tube, vibrate on the vortex mixer for 40s, then ultrasonic water bath for 15min, take it out and continue to vibrate on the vortex mixer for 40s, then centrifuge at 2200rpm, room temperature 20-25℃ for 6min , to obtain the supernatant. Draw 0.3mL of the supernatant with a glass dropper, dilute the supernatant 40 times with absolute ethanol by volume as the test solution, and dilute 40 times with an equal volume of n-hexane with absolute ethanol as the Reference solution, utilize 752 type ultraviolet-visible spectrophotometers to measure under 225nm wavelength, the OD value that records three times is all greater than 1, then the soluti...

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Abstract

The invention relates to a quick measuring method of pyrethrin, which can quickly and effectively measure the content of the pyrethrin in dried flowers. The method comprises the following steps of: picking up 10-20 capitulums when tubular pyrethrum flowers bloom by 50-99 percent, and placing the capitulums into an oven at 40-80 DEG C for drying for 36-72 hours; pulverizing, then weighing 50-200mg of powder and putting the weighed powder into a glass centrifuge tube; adding 2-10mL of normal hexane, covering the glass centrifuge tube and then oscillating for 20-40s on a vortex mixer; placing the glass centrifuge tube in an ultrasonic water bath for 5-15min, taking out and then continuing to oscillating for 20-40s; centrifuging for 3-8min at a speed of 1,500-3,000 turns / min at room temperature of 20-25 DEG C; absorbing 0.1-0.5mL supernate, and metered by the volume, diluting for 40-80 times with absolute ethyl alcohol as a solution to be measured; measuring the light absorbing valve by an ultraviolet and visible spectrophotometer under the condition of a wavelength of 225nm; and calculating the content of the pyrethrin. The invention has the advantages of simple pretreatment, low testing cost, high speed and good accuracy and can improve the field breeding measuring efficiency and the selecting efficiency of good genotypes.

Description

technical field [0001] The invention relates to a method for measuring pyrethrin content in dried pyrethrum flowers. The method can quickly and effectively determine the content of pyrethrum in large quantities, improve the efficiency of field breeding of pyrethrum, and lay a foundation for accelerating the process of selection and popularization of pyrethrum improved varieties. Background technique [0002] Pyrethrum (Tanacetum cinerariifolium, syn. Chrysanthemum cinerariifolium, Pyrethrum cinerariifolium) is a perennial herbaceous plant of Asteraceae, and the pyrethrum with insecticidal activity isolated from its flower head is a general term for six chemical components. Pyrethrins can quickly knock down animals such as insects and mosquitoes, and are non-toxic and harmless to mammals and plants. At the same time, they will slowly decompose into water and CO in the natural environment. 2 Therefore, it is internationally recognized as the non-polluting, safe and pollution-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/34
Inventor 王彩云李杰李雅菲曹丽园毛静袁芳
Owner HUAZHONG AGRI UNIV
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