Rapid determination method of content of soluble proteins
A technology for rapid determination of protein content, which is applied in measurement devices, preparation and sampling of samples for testing, etc., can solve problems such as decline, and achieve the effects of accurate determination results and simple determination methods.
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[0022] Example 1
[0023] (1) Preparation of protein suspension: Add distilled water at a 4% material-to-liquid ratio and mix evenly, that is, weigh 120 g of rice protein and add 3L of distilled water to make a suspension.
[0024] (2) Ultrasound-assisted extraction of protein: Single-frequency focused ultrasonic equipment is used to assist the extraction of rice protein. The 28 kHz ultrasonic probe is completely immersed in the rice protein solution, and the ultrasonic parameters are set: power density 60 W / L, intermittent ratio 6:6 s , The temperature is 30℃, and the time is 41 min. Use an automatic titrator to add 0.05 M NaOH dropwise to maintain the pH in the reaction solution. Before the start of ultrasound and at the 5th minute after the ultrasound, record the amount of alkali added every 3 minutes, and sample 1 ml at the same time at 5000 r / Centrifuge under min conditions, and determine the protein content of the supernatant by Lowry method.
[0025] (3) Establish a mathema...
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[0029] Example 2
[0030] (1) Preparation of protein suspension: Add distilled water at 15% material-to-liquid ratio and mix evenly, that is, weigh 450 g of rice protein and add 3L of distilled water to make a suspension.
[0031] (2) Ultrasound-assisted extraction of protein: Single-frequency focused ultrasonic equipment is used to assist the extraction of rice protein. The 28 kHz ultrasonic probe is completely immersed in the rice protein solution, and the ultrasonic parameters are set: power density 60 W / L, intermittent ratio 6:6 s , The temperature is 30℃, and the time is 41 min. Use an automatic titrator to add 0.06 M NaOH dropwise to keep the pH in the reaction solution constant. Before the start of ultrasound and at the 5th minute after the ultrasound, record the amount of alkali consumed every 3 minutes, and sample 1 ml at 5000 r / Centrifuge under min conditions, and determine the protein content of the supernatant by Lowry method.
[0032] (3) Establish a mathematical mode...
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