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Determining method and device of nicotine in oral smokeless tobacco products

A smokeless tobacco and measurement method technology, applied in the field of new tobacco detection, can solve the problems of cumbersome experimental process, difficulty in controlling human injury, and lack of commercialized professional instruments, etc., achieving good selectivity, easy promotion, and simple measurement method Effect

Inactive Publication Date: 2014-01-29
YUNNAN RES INST OF TOBACCO SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The experimental results of this method are easily affected by the individual physiological differences of the volunteers and the habit of consuming tobacco products. The experimental process is cumbersome, difficult to control, and relatively harmful to the human body.
[0006] The other is an in vitro experiment, which simulates the entire dynamic process of oral consumption of smokeless tobacco products by the human body, and collects the nicotine release liquid in real time for quantitative analysis and detection. Currently, there is no commercialized professional instrument, and a self-made simulation device is required for research.

Method used

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  • Determining method and device of nicotine in oral smokeless tobacco products
  • Determining method and device of nicotine in oral smokeless tobacco products
  • Determining method and device of nicotine in oral smokeless tobacco products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Test sample: bulk smokeless tobacco product Copenhager 34g (Altria). Weigh 1.0 g of Copenhager smokeless tobacco products, place in dissolution tank 5, artificial saliva (formula: K2HPO42.5mmol / L, NaH2PO42.4mmol / L, KHCO315.0mmol / L, NaCl10.0mmol / L, MgCl21. 5mmol / L, CaCl21.5mmol / L, Citric Acid0.15mmol / L), the pH value is 6.8, and the flow rate of the constant flow pump 3 is set to 2.0mL / min. The speed is about 0.06-1.8mL / min, and the saliva secretion speed can reach 7.0mL / min under strong stimulation. For the convenience of research, the present invention chooses a flow rate of 2.0mL / min for the in vitro release experiment of nicotine. The test was carried out for 60 minutes, and the nicotine release liquid was collected every 5 minutes. At 60 minutes, the system automatically stopped, and a total of 12 release liquid samples were collected. Take out the residue, put it into a 100mL Erlenmeyer flask with a stopper, add 10mL of the above-mentioned artificial saliva, cover...

Embodiment 2

[0031] Test sample: bulk smokeless tobacco product Grizzly 34g (Reynolds). Weigh 1.0 g of Grizzly smokeless tobacco product and place it in the dissolution tank 5, the artificial saliva (the formula is the same as in Example 1) has a pH value of 6.8, and the flow rate of the constant flow pump 3 is set to 2.0 mL / min. The test was carried out for 30 minutes, and the nicotine release liquid was collected every 2 minutes. The system automatically stopped at 30 minutes, and a total of 15 release liquid samples were collected. Take out the residue, put it into a 100mL Erlenmeyer flask with a stopper, add 10mL of the above-mentioned artificial saliva, cover the stopper, shake it on a shaker for 20min, centrifuge, and take the supernatant for later use.

[0032] The detection of nicotine in the release liquid and the residue extract was performed by high performance liquid chromatography, and the chromatographic conditions were the same as in Example 1. Calculate the cumulative rele...

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Abstract

The invention relates to a determining method and device of nicotine in oral smokeless tobacco products, and belongs to the technical field of novel tobacco detection. The method comprises the following steps of putting a piece of piled filtration paper into a dissolution pool (5), wetting by artificial saliva, weighing 0.5-2.0g of oral smokeless tobacco products, filling into the dissolution pool (5) which is filled up with the piece of filtration paper, placing a collection bottle (6) below the dissolution pool (5), regulating the height of a support (7), and closing a door of a constant-temperature box (4) for dissolution test; and analyzing a release liquid which is collected in real time quantitatively by a high performance liquid chromatograph. An in-vitro dynamic release device consists of a constant-temperature water bath pot (1), a solvent storage bottle (2), a constant-flow pump (3), the constant temperature box (4), the dissolution pool (5), the collection bottle (6) and the support (7). The method provided by the invention has the advantages that operation is simple, convenient and rapid, and selectivity is good; the device has the advantages of simple structure and time and labor conservation, and is convenient to dismount and clean.

Description

Technical field: [0001] The invention relates to a method and a device for measuring nicotine in inhaled smokeless tobacco products, belonging to the technical field of new tobacco detection. Background technique: [0002] Intake-type smokeless tobacco products mainly include buccal-type smokeless tobacco products and chemical-type smokeless tobacco products. The biggest difference between these products and traditional cigarettes is that these products are smokeless tobacco products. When consumers use it like eating candy, through ingestion, the nicotine and other tobacco components contained in this new type of tobacco product are dissolved in saliva and then absorbed by the body through the oral mucosa, and finally give consumers a certain sense of physiological satisfaction. , Too addicted to smoking. [0003] In recent years, due to restrictions on smoking in public places around the world, the consumption of smokeless tobacco products has been increasing year by year...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 陈建华耿永勤杨叶昆李晶缪恩铭魏玉玲徐济仓李雪梅缪明明
Owner YUNNAN RES INST OF TOBACCO SCI
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