Method for determining pollen number in rice anther
A technology for pollen and anther, applied in the field of rice crop planting research and application, can solve the problems of difficulty in measuring single pollen quality, error in pollen photo counting, difficulty in accurate counting, etc., and achieves the effects of convenient measurement method, reliable data and high measurement efficiency.
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[0024] The implementation of the present invention can be carried out according to the following steps:
[0025] (1) The production of reticle:
[0026] The reticle is made of a glass slide with a length of 73mm, a width of 38mm, and a thickness of 1mm; at the center of the reticle, 168 grids of 2280μm×3048μm are made with a metal wire; the line width accuracy of the grid is 6±1.5μm, the line height does not exceed 2μm.
[0027] (2) Powder taking and film making:
[0028] Sample sampling: Take the rice spikelets that will open the next day, place them in a petri dish, and then put them in an ice box to moisturize and bring them back to the laboratory; or pull out the whole ear from the inverted section, remove the flag leaves and put them in Take the bottle with water back to the laboratory. Drop 1-2 drops of I with a concentration of 0.1%-0.3% on the reticle 2 -KI solution, and then take 1 anther medicinal forceps from a spikelet in I 2 -Repeatedly squeeze different positions in th...
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