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Reagent for determining fibrinogen content without need of diluting plasma sample and the determination method

A fibrinogen and reagent technology, applied in biological testing, material testing, etc., can solve the problems of high cost of unsolved FIB reagents, avoid changes in physiological environment, reduce thrombin dosage, and improve accuracy.

Active Publication Date: 2007-10-24
BAODING TIANYUE BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the law still does not solve the high cost of FIB reagents

Method used

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  • Reagent for determining fibrinogen content without need of diluting plasma sample and the determination method

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Experimental program
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Effect test

Embodiment 1

[0082] In embodiment 1, with a kind of stabilizing agent;

Embodiment 2

[0083] In embodiment 2, with 2 kinds of stabilizing agents;

Embodiment 3

[0084] In embodiment 3, with 3 kinds of stabilizing agents;

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Abstract

The invention relates to an agent which can test the fiber proteinogen content without diluting plasma sample, and a relative test method, wherein the agent comprises 2-20 units of thrombase in each milliliter agent, 5-100 mill mol buffer agents in each liter agent, 0. 05-10g in each 100g milliliter agent, and the left is deionized water. And the test method comprises that (1), using the buffer liquid with stabilizer to prepare the agent which contains 2-20 units of thrombase in each milliliter, (2), a, cultivating the agent and the plasma sample at 37Deg. C, to be mixed in the volume ratio as 1:1, b, recording the blood coagulation time, c, using a standard curvature to obtain the fiber proteinogen content of the sample. The invention can avoid diluting sample in test, with simple test method, reduced agent production cost, improved test accuracy and reduced operation error.

Description

technical field [0001] The invention relates to a reagent for determining the content of fibrinogen (FIB) in a plasma sample and a determination method thereof, and belongs to the technical field of clinical detection reagents and detection methods. Background technique [0002] Quantitative determination of plasma fibrinogen (FIB) is one of the four blood coagulation items (ie PT, TT, FIB and APTT) widely performed clinically. The von Clauss (von Clauss) FIB assay belongs to the functional assay and is recommended by the National Committee for Clinical Laboratory Standards (NCCLS). The principle of this method is that high-concentration thrombin reacts with low-concentration FIB plasma, and the FIB content is inversely proportional to the coagulation time. The FIB content of the plasma to be tested can be found out by a standard curve made on double-logarithmic coordinate paper. However, this method has two disadvantages: [0003] 1. A high conc...

Claims

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Application Information

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IPC IPC(8): G01N33/86
Inventor 静天玉
Owner BAODING TIANYUE BIOENG
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