33 results about "Naked mole-rat" patented technology

The invention belongs to the technical field of cell culture, and particularly relates to a culture method for a heterocephalus glaber cardiac muscle cell. In the culture method, the operation steps of separating and purifying the heterocephalus glaber cardiac muscle cell are simple, cell attachment is fast, the survival rate is high, and a cell culture system is high in stability. The culture method is an ideal culture method for the heterocephalus glaber primary cardiac muscle cell, can basically meet the requirements for heterocephalus glaber cardiac muscle cell related experiment research in all research fields such as medical science, biology, pharmacy and bioscience and is suitable for application and popularization in general laboratories.

The invention relates to the technical field of cell biology, in particular to a method for separating, purifying and culturing naked mole-rat Schwann cells. The invention comprehensively uses various mediums to separate and purify Schwann cells from the DRG ganglion of the spinal cord of naked mole rat fetal rats, and finds out a reasonable culture method for naked mole rat Schwann cells suitable for temperature-changing rodent mammals. The method of the present invention can obtain a large number of naked mole-rat Schwann cells with normal functional activity in a simple, efficient and economical manner, and the culture under hypoxic conditions can ensure that the biological characteristics of the cells in the in vitro environment can still be maintained. , so that it is convenient to further study the special physiological functions of naked mole rat Schwann cells directly in a pure in vitro cell culture model, thereby providing an important theoretical basis for exploring the biological mechanism and applying it to clinical related fields.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat astroglia cells. The astroglia cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat astroglia cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat astroglia cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat astroglia cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the field of immunoprecipitation, in particular to a method for ultrasonically disrupting naked mole-rat tissue in chromatin immunoco-precipitation. The ultrasonically disrupting method is to firstly add collagen with a volume ratio of 1:1:1 to the naked mole-rat tissue The mixed solution of enzyme II solution, hyaluronidase solution, and DPBS buffer was shaken at 37°C to digest the tissue, centrifuged to remove the supernatant, and then the following conventional ultrasonic disruption steps were performed. Using this method can better obtain DNA-protein complexes with appropriate fragment sizes, laying a good foundation for the success of the entire ChIP experiment later.

The invention relates to the field of in vitro culture of cells, in particular to a method for separating and culturing naked mole-rat skeletal muscle myoblasts. The naked mole-rat is obtained by using trypsin combined with collagenase and neutral protease to digest and separate, and filtering and differentially adhering to the wall Skeletal muscle myoblasts. The present invention establishes a set of methods for isolating, cultivating and identifying naked mole-rat skeletal muscle myoblasts, and evaluates the maintenance of the biological characteristics of the cells cultured by this method, confirming that this culture method can obtain naked mole rat skeletal myoblasts, which provides the possibility to further study the functional changes of naked mole rat skeletal myoblasts under hypoxic physiological conditions.

The invention relates to the field of stem cell culture, in particular to a method for primary isolation and culture of naked mole rat bone marrow mesenchymal stem cells, comprising the following steps: 1) washing the naked mole rat bone marrow; 2) passing the bone marrow flushed out through a cell sieve; 3) Destroy red blood cells; 4) direct plating. The present invention establishes a method for isolating and culturing naked mole rat bone marrow mesenchymal stem cells, and evaluates the biological characteristics of the cells cultured by the method, confirming that the culture method can obtain naked mole rat bone marrow with high purity and differentiation ability Mesenchymal stem cells provide the possibility to further study the characteristics of naked mole rat stem cells and explore the characteristics of naked mole rat somatic cells that are not easy to separate but can be transformed by stem cells.

The invention relates to the field of cell culture, in particular to a method for separating and purifying naked mole-rat testicular interstitial cells, which adopts enzyme digestion and a Percoll continuous density gradient method to separate and purify naked mole-rat testicular interstitial cells. The method of the present invention can obtain a large number of naked mole rat testicular stromal cells with normal functional activity in a simple, efficient and economical manner, and the culture under hypoxic conditions can ensure that the cells can still maintain their biological properties in an in vitro environment. characteristics, so that it is convenient to further study the special physiological functions of naked mole rat Leydig cells directly in a pure in vitro cell culture model, thus providing an important theoretical basis for exploring the biological mechanism and applying it to clinical related fields.

The invention relates to the technical field of animal breeding and specifically relates to a method for breeding and pairing naked mole rats. According to the method for breeding and pairing the naked mole rats, provided by the invention, from the weight pairing for the parent naked mole rats to the selection for the mating time and from the single cage breeding before the mating to the body odor mixing, a series of methods for increasing the success rate of the mating of the naked mole rats are formed. These methods can be used for overcoming the mating barrier of the naked mole rats and can supply key technical support for establishing a closed group of the naked mole rats.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat cortical neurons. The cortical neurons are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat cortical neurons is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat cortical neurons with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat cortical neurons can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the field of experimental animal production, and in particular relates to a superovulation method of naked mole rats. The superovulation method comprises the following steps of: at the 1st, 3th, 5th and 7th days after the oestrus is ended, injecting PMSG (Pregnant Mare Serum Gonadotropin) into the abdominal cavities of the female naked mole rats; at the 7th day, injecting HCG with the dosage of 0.12IU / g (body weight) into the abdominal cavities of the naked mole rats; at the time of 1 hour after injection of HCG, starting to cage the female naked mole rats and male rats with fertility for free mating. The superovulation method determines the implementation schedule, the drug ingredients and dosage by verification of numerous tests. The superovulation method has the advantages that a set of superovulation program for the naked mole rats is made, so that the blank that the superovulation program is not made temporarily after introduction of the naked mole rats in China is filled; every experimental animal responses to the superovulation and can form 51-55 follicles. The adopted drugs and program are more suitable for the naked mole rats, and the superovulation program is more economical.

The invention discloses an application of naked mole rat giant molecular weight hyaluronic acid in the preparation of drugs for treating breast cancer, and relates to the application of the naked mole rat giant molecular weight hyaluronic acid. The prepared giant molecular weight hyaluronic acid belongs to the integral components in human body tissues and does not cause harm to patients; prepared hydrogel by using the giant molecular weight hyaluronic acid can achieve local delayed-release treatment, and is functional only for breast cancer lesion sites without hurting other normal tissues, and the hydrogel has no side effects, the operation is simple. The application of the naked mole rat giant molecular weight hyaluronic acid in the preparation of the drugs for treating breast cancer relates to the field of pharmaceuticals.

The invention relates to the technical field of cell biology, in particular to a separation, purification and culture method of naked mole rat hippocampal neurons. The hippocampal neurons are separated from newly-born naked mole rat cerebral cortices and purified and cultured by the aid of multiple culture media comprehensively, and a reasonable culture method suitable for poikilothermal rodent mammal naked mole rat hippocampal neurons is found out. With the adoption of the method, a large number of naked mole rat hippocampal neurons with normal functional activity can be acquired conveniently, efficiently and economically, the cells can still keep biological characteristic in the in-vitro state in the in-vitro environment through culture under the low-oxygen condition, so that special physiological functions of the naked mole rat hippocampal neurons are further researched in pure in-vitro cell culture models directly and conveniently, and the important theoretical basis is provided for exploration of the biological mechanism and application to related clinical fields.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat oligodendroglia precursor cells. The oligodendroglia precursor cells are separated from cerebral cortices of naked mole rat fetal rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat oligodendroglia precursor cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat oligodendroglia precursor cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat oligodendroglia precursor cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat microglial cells. The microglial cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat microglial cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat microglial cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat microglial cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

A conditioned cell culture medium of cells derived from Spalax or naked mole rat (Heterocephalus glaber) and methods for preparing it are provided. Pharmaceutical compositions comprising the conditioned cell culture medium and its use in the treatment of cancer as well as methods for identifying anti-cancer agents are also provided.