33 results about "Naked mole-rat" patented technology

The invention relates to the field of in-vitro culture of cells and in particular relates to an isolated culture method of alveolar type II epithelial cells of naked mole rats. The isolated culture method comprises the following steps: combining pancreatin and elastase and carrying out digestion and isolation; filtering, carrying out differential adhesion and screening and purifying an IgG antibody to obtain the alveolar type II epithelial cells of the naked mole rats. The invention establishes one set of method for isolating, culturing and identifying the alveolar type II epithelial cells of the naked mole rats; a biological characteristic maintaining condition of the cells cultured by the method is evaluated, so that the culture method used for obtaining the high-purity alveolar type II epithelial cells of the naked mole rats is verified, the possibility for further deeply researching proliferation and differentiation, liquid transferring, synthesis and secretion of the alveolar type II epithelial cells, and function changes of the alveolar type II epithelial cells under pathophysiologic conditions of lung injuries and repairing and the like is provided.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat oligodendroglia precursor cells. The oligodendroglia precursor cells are separated from cerebral cortices of naked mole rat fetal rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat oligodendroglia precursor cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat oligodendroglia precursor cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat oligodendroglia precursor cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the technical field of cell biology, in particular to a separation, purification and culture method of naked mole rat hippocampal neurons. The hippocampal neurons are separated from newly-born naked mole rat cerebral cortices and purified and cultured by the aid of multiple culture media comprehensively, and a reasonable culture method suitable for poikilothermal rodent mammal naked mole rat hippocampal neurons is found out. With the adoption of the method, a large number of naked mole rat hippocampal neurons with normal functional activity can be acquired conveniently, efficiently and economically, the cells can still keep biological characteristic in the in-vitro state in the in-vitro environment through culture under the low-oxygen condition, so that special physiological functions of the naked mole rat hippocampal neurons are further researched in pure in-vitro cell culture models directly and conveniently, and the important theoretical basis is provided for exploration of the biological mechanism and application to related clinical fields.

The invention relates to the field of stem cell culture, and particularly discloses a primary separation culture method for bone marrow mesenchymal stem cells of naked mole rats. The primary separation culture method includes steps of 1), washing bone marrow of the naked mole rats; 2), sieving bone marrow washed substances by the aid of cell strainers; 3), breaking red blood cells; 4), carrying out direct plate laying. The primary separation culture method for the bone marrow mesenchymal stem cells of the naked mole rats has the advantages that the primary separation culture method is established for the bone marrow mesenchymal stem cells of the naked mole rats, biological characteristics of the bone marrow mesenchymal stem cells cultured by the aid of the primary separation culture method are evaluated, the fact that the bone marrow mesenchymal stem cells with extremely high purity and differentiation capacity can be obtained by the aid of the primary separation culture method is proved, and accordingly intensive study on characteristics of stem cells of the naked mole rats and exploration on characteristics of somatic cells, which are difficult to separate but can be obtained from the stem cells by means of transformation, of the naked mole rats can be possibly carried out.

This invention provides cell culture compositions which produce significant quantities of high molecular weight hyaluronic acid. The cell cultures are obtained from cells of mole rats, such as naked mole rats and blind mole rats. The high molecular weight hyaluronic acid can be collected in the conditioned media of these cell cultures. These cell cultures provide a convenient source of large quantities of high molecular weight hyaluronic acid.

The invention relates to the field of cell cultivation and in particular relates to a separation and purification method of testicular interstitial cells of a naked mole rat. The interstitial cells of the naked mole rat are separated and purified by adopting enzymatic digestion and a Percoll continuous density gradient method. The method provided by the invention can obtain a lot of testicular interstitial cells of the naked mole rat with normal functional activity simply, efficiently and economically, and cultivation under a low oxygen condition can ensure that the cells still can keep the biological characteristics in an in-vivo state in an in-vitro environment, so that the special physiological functions of the testicular interstitial cells of the naked mole rat can be conveniently and directly researched in a pure in-vitro cell cultivation model further, thereby providing an important theoretical basis for exploring the biological mechanism and applying the same to the clinical related fields.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat cortical neurons. The cortical neurons are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat cortical neurons is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat cortical neurons with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat cortical neurons can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the field of experimental animal production, and in particular relates to a superovulation method of naked mole rats. The superovulation method comprises the following steps of: at the 1st, 3th, 5th and 7th days after the oestrus is ended, injecting PMSG (Pregnant Mare Serum Gonadotropin) into the abdominal cavities of the female naked mole rats; at the 7th day, injecting HCG with the dosage of 0.12IU/g (body weight) into the abdominal cavities of the naked mole rats; at the time of 1 hour after injection of HCG, starting to cage the female naked mole rats and male rats with fertility for free mating. The superovulation method determines the implementation schedule, the drug ingredients and dosage by verification of numerous tests. The superovulation method has the advantages that a set of superovulation program for the naked mole rats is made, so that the blank that the superovulation program is not made temporarily after introduction of the naked mole rats in China is filled; every experimental animal responses to the superovulation and can form 51-55 follicles. The adopted drugs and program are more suitable for the naked mole rats, and the superovulation program is more economical.

The invention relates to the technical field of animal breeding and specifically relates to a method for breeding and pairing naked mole rats. According to the method for breeding and pairing the naked mole rats, provided by the invention, from the weight pairing for the parent naked mole rats to the selection for the mating time and from the single cage breeding before the mating to the body odor mixing, a series of methods for increasing the success rate of the mating of the naked mole rats are formed. These methods can be used for overcoming the mating barrier of the naked mole rats and can supply key technical support for establishing a closed group of the naked mole rats.