7 results about "Epitheliocyte" patented technology

The invention provides an application and a method for inducing phenotype transformation from oral mucosa epithelium to dry lip epithelium by linoleic acid, linoleic acid with proper concentration acts on oral mucosa epithelial cells to regulate and control cell differentiation procedures, so that expression of specific markers of the oral mucosa epithelial cells is reduced, expression of specific markers of dry lip epithelial cells is increased, and expression of the specific markers of the oral mucosa epithelial cells is increased. The directional transformation of the cell phenotype is realized. The linoleic acid with the proper concentration is 10-20 [mu] M. The linoleic acid has the effects of regulating keratinization index expression of mucous membrane epithelial cells and enhancing the mucous membrane tissue barrier function for the first time, and the function is not recognized in previous research and clinical application of the linoleic acid.

The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof, and relates to the technical field of medicines.The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof.Firstly, a mixed cell group of the vaginal epithelium containing a complete cell lineage and capable of long-term subculture is successfully established, a technical bottleneck that primary vaginal epithelial cells are difficult to expand is solved, and ideal seed cells are provided for model construction.Secondly, a highly-bionic vaginal mucosa model having a tight connection structure, rich layers and an orderly arrangement is constructed, and the physiological barrier and immune regulation functions of the vaginal mucosa are truly simulated.Finally, by introducing lactobacillus symbiosis and confirming the response to estrogen, a multi-element in-vitro research model integrating the anatomical structure of the vagina, resident flora, local immunity and endocrine regulation is constructed, and a reliable technical platform is provided for in-depth exploration of the mechanism of vaginal micro-ecological disorder and efficient screening of related drugs.

The invention discloses a method for constructing a nasal mucosa epithelium single-layer model by using a single-cell digested organoid, and belongs to the technical field of bioengineering. The method comprises the following steps: firstly, carrying out digestion treatment, red blood cell lysis treatment, inoculated culture and passage on nasal mucosa tissues to obtain nasal mucosa organoid, then adding recombinase for dissociation to obtain dissociated epithelial cells, finally, planting the dissociated epithelial cells in a culture container subjected to coating treatment for incubation and adherence, and continuing to culture to obtain the nasal mucosa organoid. And preparing the nasal mucosa epithelium single-layer model. The multi-lineage differentiation potential and individual specificity of the organoid-derived cells are reserved, the cell adherence, passage and cryopreservation recovery stability is remarkably improved, the culture period is shortened, reagent expenditure and time cost are saved, and the defects that a traditional cell line is poor in physiological correlation, difficult in primary and gas-liquid interface culture acquisition, long in period and low in cost are overcome. And the three-dimensional organ-like chamber structure is not beneficial to top surface observation and solute transport research, so that the three-dimensional organ-like chamber structure is more suitable for scientific experiments.

The present invention relates to superoxide dismutase, and uses thereof for preventing or treating mucositis. The superoxide dismutase according to the present invention can be effectively used for treating or improving the inhibition of a proliferation of mucous epithelium cells, which is a side effect of anticancer agents or radiotherapy.

The invention provides application of fludarabine in preparation of a medicine for preventing and / or treating corneal epithelium lesion, and belongs to the technical field of biological medicine preparation. The invention discloses application of fludarabine in preparation of a medicine for preventing and / or treating corneal epithelium lesion. Experiments show that diabetes can cause increase of expression of STAT1 in corneal epithelium cells and delay healing of mouse corneal epithelium wounds, and the exogenous STAT1 antagonist fludarabine can reverse the healing delay phenomenon of diabetic mouse corneal epithelium wounds and promote repair of the corneal epithelium wounds. Therefore, the technical scheme provided by the invention provides a new treatment means for repairing the corneal epithelium lesion.

This determination device comprises a processor that acquires a Raman spectrum which has been detected by irradiating a gastrointestinal mucosal tissue with excitation light having a prescribed wavelength, that calculates, from the Raman spectrum in a first wavenumber band, a first feature amount which represents the state or amount of a substance contained in the mucosal epithelium, that determines the risk of future deterioration of the gastrointestinal mucosal tissue on the basis of the first feature amount, and that outputs the result of the determination. Thus, provided is a determination device which is capable of accurately determining the risk of future deterioration of a gastrointestinal mucosal tissue.

The invention relates to a preparation process of a polydimethylsiloxane injection surface for releasing a cell sheet and application of the polydimethylsiloxane injection surface in corneal reconstruction. The abstract content is inspired by a smooth surface of a pitcher plant flesh opening to trap insects into a bag. A scientist introduces smooth liquid as an unbonded coating into the surface of a porous material to achieve the purposes of resisting pollution, preventing dust, preventing adhesion growth of marine invertebrate organisms and preventing rust, the silica gel culture dish is researched, developed and prepared by referring to related data to solve the problem of cell sheet adhesion removal by applying the technology, and after modification, film coating, inoculated culture and adhesion removal treatment, the cell sheet adhesion removal effect is improved. After many times of failures, the corneal epithelium cell sheet is removed, the cell sheet is further identified to be a cell sheet with high survival rate and regeneration capacity, and animal corneal cell sheet transplantation experiments confirm that the transplanted cell sheet can be quickly decocted with the damaged corneal epithelium, so that the corneal epithelium cell sheet can be quickly adhered to the damaged corneal epithelium, and the corneal epithelium cell sheet can be quickly adhered to the damaged corneal epithelium. And a P63 labeled protein with regeneration characteristics is observed to exist in a cornea layer through tissue slice staining.