Placenta stem cell bank construction method and placenta tissue resuscitation method

A technology of placental stem cells and placental tissue, which is applied in the field of construction of placental stem cell banks, can solve the problems that stem cells cannot be preserved, and achieve the effects of prolonging storage time, ensuring activity, and simple operation methods
CN104480533AInactive Publication Date: 2015-04-01天晴干细胞股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
天晴干细胞股份有限公司
Publication Date
2015-04-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a placenta stem cell bank construction method and a placenta tissue resuscitation method, and relates to placenta stem cell bank construction methods. The placenta stem cell bank construction method comprises the following steps: after non-bacterial cleaning treatment to placenta tissues of at least three parts, the steps of tissue peeling, further sterilizing, shearing to be thin, protecting using a refrigerant, programmed freezing, cryogenic temperature temporary storage, long term storage with nitrogen canisters, and the like are carried out, and the placenta tissue with biological activities can be preserved for a long term. The placenta tissue preserved through the method can be used for the construction of a stem cell bank, resuscitation, enzymic digestion, cultivation, expansion, quality inspection and the like are performed after selective tissue part resuscitation according to required cell types, so as to obtain placenta amniotic membrance epithelial cells, placenta amniotic membrance mesenchyme, placenta chorion mesenchymal stem cell, placenta decidua serotina mesenchymal stem cells and the like. The method provided by the invention has the advantages that operation steps are simplified, manual intervention errors are reduced, the efficiency is improved, more stem cell resources are preserved, and a basis is provided for further personalized stem cell customization.
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Description

technical field

[0001] The invention relates to a method for constructing a placental stem cell bank and a method for resuscitating placenta tissue. Background technique

[0002] The placenta is the link for the delivery of various nutrients that the baby depends on for survival, and is composed of amniotic membrane, chorion and decidua basalis. The amnion and chorion are of fetal origin, while the decidua basalis is of maternal origin. In addition to playing an important role in fetal development, nutritional support and maintenance of tolerance, the placenta is also a body's stem cell reserve.

[0003] There are many kinds of pluripotent stem cells in the placenta, and at least three types of placental stem cells are known. The first type of pluripotent stem cells in the placenta are mesenchymal stem cells (Mesenchymal stem cells, MSCs). Tissue cells such as liver cells.

[0004] The characteristics of placental MSCs that can suppress immunity and promote hematopoietic...

Claims

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