Decellularized heterogeneous corneal stroma carrier and its preparation method and application

A corneal stroma and decellularization technology, applied in the field of medical materials, can solve the problems of HIV infection, uneven cell growth, easy shedding, etc.

Active Publication Date: 2011-12-28
陕西省眼科研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the amniotic membrane is relatively thin, with a thickness of only 20 μm to 100 μm. If cells are cultured on the surface of the amniotic membra...

Method used

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  • Decellularized heterogeneous corneal stroma carrier and its preparation method and application
  • Decellularized heterogeneous corneal stroma carrier and its preparation method and application
  • Decellularized heterogeneous corneal stroma carrier and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Preparation of decellularized ostrich corneal stroma carrier

[0055] (1) Extraction and preservation of animal eyeballs: Take healthy ostrich eyeballs within 2 hours of execution in a regular and qualified slaughterhouse, rinse the eyeballs with normal saline, and place the rinsed eyeballs in a wet room at 4°C to 8°C Preserve, cut the lamellar cornea within 24 hours;

[0056] (2) Sectioning of the lamellar cornea: wash the ostrich eyeballs preserved in step (1) alternately with normal saline and normal saline containing 400U / mL tobramycin, and then operate aseptically under an operating microscope, using an 8mm-diameter Scaled trephine drilling for lamellar cornea with a thickness of 300 μm;

[0057] (3) Hypertonic solution combined with enzyme digestion to remove lamellar corneal epithelial cells and stromal cells:

[0058] 301. Put the lamellar cornea described in step (2) into a hypertonic solution (NaCl aqueous solution with a mass concentration of 12%), and soak...

Embodiment 2

[0067] Preparation of decellularized porcine corneal stroma carrier

[0068] (1) Extraction and preservation of animal eyeballs: take healthy pig eyeballs within 2 hours of slaughter in a regular and qualified slaughterhouse, rinse the eyeballs with normal saline, and place the rinsed eyeballs in a wet room at 4°C to 8°C Preserve, cut the lamellar cornea within 24 hours;

[0069] (2) Sectioning of the lamellar cornea: rinse the pig eyeballs preserved in step (1) alternately with normal saline and normal saline containing 400U / mL tobramycin, then perform aseptic operation under an operating microscope, and use a 5mm diameter Scaled trephine drilling for lamellar cornea with a thickness of 150 μm;

[0070] (3) Hypertonic solution combined with enzyme digestion to remove lamellar corneal epithelial cells and stromal cells:

[0071] 301. Put the lamellar cornea described in step (2) into a hypertonic solution (NaCl aqueous solution with a mass concentration of 3%), and soak for ...

Embodiment 3

[0079] Preparation of decellularized bovine corneal stroma carrier

[0080] (1) Extraction and preservation of animal eyeballs: Take healthy cow eyeballs within 2 hours of slaughter in a regular and qualified slaughterhouse, rinse the eyeballs with normal saline, and place the rinsed eyeballs in a wet room at 4°C to 8°C Preserve, cut the lamellar cornea within 24 hours;

[0081] (2) Sectioning of the lamellar cornea: wash the bovine eyeballs preserved in step (1) alternately with normal saline and normal saline containing 400U / mL tobramycin, then operate aseptically under an operating microscope, and use a 5mm-diameter Scaled trephine drilling for lamellar cornea with a thickness of 150 μm;

[0082] (3) Hypertonic solution combined with enzyme digestion to remove lamellar corneal epithelial cells and stromal cells:

[0083] 301. Put the lamellar cornea described in step (2) into a hypertonic solution (NaCl aqueous solution with a mass concentration of 20%), and soak for 1 da...

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Abstract

The invention discloses a decellularized heterogeneous corneal stromal carrier and its preparation method and application. The carrier is an animal lamellar cornea from which epithelial cells and stromal cells have been removed through hypertonic solution combined with enzyme digestion. The preparation method of the carrier is as follows: firstly, take Fresh animal eyeballs are aseptically operated under an operating microscope, and the lamellar cornea with a thickness of 150 μm to 400 μm is drilled with a graduated trephine drill with a diameter of 5 mm to 12 mm, and then removed under the combined action of hypertonic solution and trypsin/pancreatin substitute The cells are finally dehydrated and dried to obtain the decellularized heterogeneous corneal stroma carrier, which is stored for future use. The decellularized heterogeneous corneal stroma carrier can be used as a corneal transplant donor to directly perform therapeutic corneal transplantation, and can also be used as an artificial biological corneal scaffold to construct a full-layer or lamellar artificial biological cornea. The decellularized heterogeneous corneal stroma carrier prepared by the invention has the following characteristics: the collagen is neatly arranged, similar to normal corneal tissue, and has good transparency after rehydration.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a decellularized heterogeneous corneal stroma carrier and its preparation method and application. Background technique [0002] According to WHO statistics in 1991, there are about 10 million corneal blindness caused by corneal disease and eye trauma in the world, and about 1.5 million to 2 million new cases are added every year. In my country, there are about 4 million blind people with corneal disease. Corneal transplantation is the only means of recovery for blind patients due to corneal disease. Due to the shortage of corneal donor materials, at present, only less than 1% of corneal transplants can be performed in my country every year. Therefore, how to obtain suitable materials for corneal transplantation has become an urgent problem to be solved in the field of ophthalmology. [0003] With the development of tissue engineering, the research of arti...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 刘先宁朱秀萍吴洁杨华银勇潘士印肖湘华
Owner 陕西省眼科研究所
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