Method for inducing embryonic calluses of large-fruit camellia chekiangoleosa

A technology of embryogenic callus and safflower camellia, which is applied in the field of plant cultivation, can solve the problems of germplasm degradation, difficulty in rooting, and difficulty in differentiating and regenerating plants, so as to reduce the degree of browning, avoid difficulty in disinfection, and facilitate disinfection treatment Effect

Inactive Publication Date: 2020-11-10
重庆福林农业生物技术研究院有限公司
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  • Description
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AI Technical Summary

Problems solved by technology

However, the traditional breeding method has a long cycle, serious germplasm degradation, slow proliferation of callus of Camellia oleifera during the cultivation process, and is not easy to differentiate and regenerate plants; the regenerated plants are cultured in vitro by using the stem section of Camellia oleifera with buds as explants, Its bud multiplication coefficient is low and rooting is difficult, resulting in the inability to realize the industrial production of Camellia oleifera, which has become a limiting factor for the industry of Camellia oleifera

Method used

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  • Method for inducing embryonic calluses of large-fruit camellia chekiangoleosa

Examples

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Embodiment 1

[0039] Inducing Embryogenic Callus of Camellia oleifera

[0040] 1) Selection and treatment of explants: In December, take a healthy and disease-free safflower oleifera tree with new buds that year, remove the buds, use them as explants, and rinse them under tap water for 30 minutes. Drain the water, peel off the scales of the bud surface, pretreat it with 75% alcohol in an ultra-clean workbench for 30 seconds and rinse with sterile water for 3-4 times, and then disinfect with 0.1% (w / v) mercury chloride solution for 1 min. After rinsing with sterile water for 3-4 times, absorb the water with sterile filter paper, cut the old wound, and set aside.

[0041] 2) Embryogenic callus induction: The medium formula for embryogenic callus induction is: 1 / 2MS+6-BA2.0mg / L+NAA2.0mg / L, sucrose 30g / L, agar 3.0g / L, PVP2.0g / L, pH=5.4. The preparation method is: add 2.215g MS medium, 2.0mg 6-BA, 2.0mg NAA, 30g sucrose, 3.0g agar, 2.0g PVP per liter of pure water, and adjust the pH value to 5.4 w...

Embodiment 2

[0046] This example is used to compare the effects of different light conditions on the induction of embryogenic callus of Camellia oleifera, and the details are as follows:

[0047] According to the operating method of Example 1, the explants were inoculated into the induction medium and then cultured under two light conditions. The first is direct light culture, the light time is 12h / d, and the light intensity is 1200lux; the second is dark culture treatment for 7 days, and then the explant material is light-cultured, the light time is 12h / d, the light intensity 1200lux.

[0048] It can be seen from Table 1 that after the explants were inoculated, the light intensity was 1200lux, 12h / d, and the embryogenic callus appeared earlier and the induction rate was high. After the explants were inoculated, they were cultured in the dark for 7 days, followed by light culture, which had a slightly lower induction rate of embryogenic callus of Camellia oleifera. Therefore, after inoculatio...

Embodiment 3

[0052] This example is used to compare the effects of different hormone concentrations on the induction of embryogenic callus of Camellia oleifera, and the details are as follows:

[0053] According to the operating method of Example 1, the explants were treated and inoculated on induction medium supplemented with different 6-BA and NAA concentrations. Except for the different concentrations of 6-BA and NAA, the other components of the medium are the same: add 2.215g MS medium, 30g sucrose, 3.0g agar, 2.0g PVP per liter of pure water, and use 1M HCl solution or 1M for pH. Adjust the NaOH solution to 5.4. A total of 6 treatments were set up, and the culture conditions were the same as in Example 1. The statistical results after 4 weeks are shown in Table 2.

[0054] Table 2. Effects of different hormone concentrations on the induction of embryogenic callus of Camellia oleifera

[0055]

[0056] It can be seen from Table 2 that treatment No. 6 has the best effect on the induction of ...

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Abstract

A method for inducing embryonic calluses of large-fruit camellia chekiangoleosa comprises the following steps of (1) selecting tender shoots on newly-drawn branches in the same year as explants, carrying out disinfection treatment, and cutting off old wounds for later use; 2) inoculating the explant subjected to disinfection treatment in the step 1) into an induction culture medium for induction culture for 3-4 weeks to obtain the embryonic calluses; and 3) transferring the embryogenic callus obtained in the step 2) into a proliferation culture medium for proliferation culture, and performingsubculture once every three weeks to obtain a large number of embryogenic tissues. The method for inducing is convenient and simple to operate, can quickly achieve induction and proliferation to obtain the large number of embryogenic calluses, and provides a basis for industrial production of the large-fruit camellia chekiangoleosa.

Description

Technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for inducing embryogenic callus of safflower oil tea. Background technique [0002] Camellia oleifera is a unique woody edible oil tree species in my country, which plays an important role in maintaining the balance of edible vegetable oil production in China and improving the structure of people's edible oil. The content of oleic acid is an important basis for evaluating the nutritional level of edible oil and an important factor for improving the physiological functions of the human body. The content of unsaturated fatty acids in tea oil is as high as 90, which is nearly 7% higher than olive oil. It is not only anti-oxidant and storage-resistant, but also has obvious effects on reducing cholesterol, anti-cancer and delaying brain aging. It is listed as a healthy edible oil that is promoted by the International Food and Agriculture Organization. [0003] Camellia semiserrata...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 赵静珂刁英
Owner 重庆福林农业生物技术研究院有限公司
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