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Mesenchymal stem cell culture medium

A technology of stem cells and culture medium, applied in the direction of cell culture active agent, cell culture support/coating, culture process, etc. The effect of a good growing environment

Pending Publication Date: 2021-04-06
任建华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Initially, researchers hoped to directly purify various nutrients and growth factors from serum, but due to the complexity of serum and the limitations of technical means, this method could not meet the actual needs

Method used

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  • Mesenchymal stem cell culture medium
  • Mesenchymal stem cell culture medium
  • Mesenchymal stem cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Preparation of mesenchymal stem cell culture medium

[0045] A mesenchymal stem cell culture medium, consisting of basal medium and additives, the basal medium is DMEM medium, and the additives are non-essential amino acids, pH regulators, antibiotics, organic substance components that promote cell membrane synthesis, cell Matrix components, cell growth factors, vitamins, trace elements and antioxidants.

[0046] Wherein, the basal medium is a DMEM liquid medium, and the consumption of the DMEM liquid medium is 500ml; the non-essential amino acid is a MEM NEAA non-essential amino acid solution, and the volume ratio of the MEM NEAA non-essential amino acid solution to the basal medium is 1 %; the pH regulator is HEPES, and the volume ratio of HEPES to basal medium is 1%; the volume ratio of the antibiotic to basal medium is 1%.

[0047] The composition of organic substances that promote cell membrane synthesis and the content in the mesenchymal stem cell cult...

Embodiment 2

[0052] Embodiment 2: Preparation of mesenchymal stem cell culture medium

[0053] A mesenchymal stem cell culture medium, consisting of basal medium and additives, the basal medium is DMEM medium, and the additives are non-essential amino acids, pH regulators, antibiotics, organic substance components that promote cell membrane synthesis, cell Matrix components, cell growth factors, vitamins, trace elements and antioxidants.

[0054] Wherein, the basal medium is a DMEM liquid medium, and the consumption of the DMEM liquid medium is 500ml; the non-essential amino acid is a MEM NEAA non-essential amino acid solution, and the volume ratio of the MEM NEAA non-essential amino acid solution to the basal medium is 1 %; the pH regulator is HEPES, and the volume ratio of HEPES to basal medium is 1%; the volume ratio of the antibiotic to basal medium is 1%.

[0055] The composition of organic substances that promote cell membrane synthesis and the content in the mesenchymal stem cell c...

Embodiment 3

[0060] Example 3: Preparation of mesenchymal stem cell culture medium

[0061] A mesenchymal stem cell culture medium, consisting of basal medium and additives, the basal medium is DMEM medium, and the additives are non-essential amino acids, pH regulators, antibiotics, organic substance components that promote cell membrane synthesis, cell Matrix components, cell growth factors, vitamins, trace elements and antioxidants.

[0062] Wherein, the basal medium is a DMEM liquid medium, and the consumption of the DMEM liquid medium is 500ml; the non-essential amino acid is a MEM NEAA non-essential amino acid solution, and the volume ratio of the MEM NEAA non-essential amino acid solution to the basal medium is 1 %; the pH regulator is HEPES, and the volume ratio of HEPES to basal medium is 1%; the volume ratio of the antibiotic to basal medium is 2%.

[0063] The composition of organic substances that promote cell membrane synthesis and the content in the mesenchymal stem cell cultur...

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Abstract

The invention belongs to the technical field of biology, and particularly discloses a mesenchymal stem cell culture medium. The mesenchymal stem cell culture medium comprises a basal culture medium and additives, wherein the additives comprise non-essential amino acids, a pH regulator, antibiotics, organic substance components for promoting cell membrane synthesis, cell matrix components, cell growth factors, vitamins, trace elements and an antioxidant. The mesenchymal stem cell culture medium can provide nutrient substances required by cell growth for stem cell growth, maintain the cell growth environment and the cell activity, promote stem cell division and proliferation, increase the stem cell proliferation speed and shorten the stem cell culture time; meanwhile, stem cell differentiation can be inhibited, but the differentiation potential of stem cells is not influenced; after the culture medium is used for carrying out passage on the stem cells for multiple times, the stem cells can still keep the original biological characteristics and multidirectional differentiation potential.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a medium for mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells derived from the mesoderm, capable of unlimited self-renewal and multidirectional differentiation. Under appropriate conditions, it can be differentiated into fat cells, chondrocytes, muscle cells, etc. in vitro or in vivo, as seed cells for tissue repair. When used in organ transplantation, it can reduce immune rejection, improve the success rate of transplantation, and speed up the recovery of surgical wounds. Mesenchymal stem cells can be cultured and expanded in vivo or in vitro, and can secrete a large number of cytokines, have functions such as immune regulation and inflammation suppression, and are used for the treatment of immune-related diseases. Moreover, mesenchymal stem cells are basically non-immunogenic and can be transplanted allogeneically. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N5/0667C12N5/0668C12N2500/32C12N2500/60C12N2500/36C12N2500/46C12N2500/30C12N2533/52C12N2533/50C12N2501/105C12N2501/11C12N2501/12C12N2501/115C12N2501/119C12N2501/165C12N2501/15C12N2501/235C12N2501/125C12N2501/10C12N2501/135C12N2500/25C12N2501/734C12N2501/14C12N2501/21C12N2501/2302C12N2501/2308C12N2501/22C12N2501/998C12N2500/44C12N2500/38C12N2500/16C12N2500/22C12N2500/10C12N2509/00C12N2509/10
Inventor 任建华熊义
Owner 任建华
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