Rapid propagation method for stem segments of Dendrobium huoshanense
A technology for Dendrobium huoshanensis and stem segments, which is applied in the field of rapid propagation of Dendrobium huoshanense stem segments, can solve the problems of low reproduction rate of Dendrobium huoshanense, high prices of Dendrobium huoshanense seedlings, reduction of wild resources, etc., and achieves guaranteed medicinal value and simple and easy reproduction method. The effect of the line, the process is simple
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[0019] Example 1
[0020] A method for rapid propagation of Dendrobium huoshanense stems includes the following steps:
[0021] (1) Preparation of explant
[0022] Select the annual stems of Dendrobium huoshanense with good quality, strong growth, obvious stem nodes, and no pests and diseases as explants, rinse the explants with running water, and then soak the explants with 75% alcohol on a sterile operating table 30 Second, sterilize with 0.1% mercury solution for 15 minutes, then rinse with sterile water 5 times, take sterile filter paper to absorb water on the surface of explants, and cut into at least one stem section under aseptic conditions Stem segment
[0023] (2) Induction culture
[0024] The stems were inoculated in a sterile induction medium. The formula of the induction medium was: N6+2.5mg / L6-BA+0.75% agar+3% sucrose+8% coconut juice+0.4% activated carbon, and the pH value was 5.4~ 5.9; The cultivation conditions are temperature 25℃, light time 12 hours / day, light inte...
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