3 results about "Intercellular connection" patented technology

The application discloses a KEGG pathway constrained dynamic graph structure optimization cross-patient cell annotation method, comprising the following steps: obtaining and preprocessing single-cell RNA sequencing data of a reference patient and a query patient; calculating a functional activity score of cells based on a KEGG pathway classification system, and constructing a KEGG function semantic matrix; constructing an initial sparse kNN cell graph; using the KEGG function semantic vector as a biological constraint, dynamically adjusting the connection weight between cells through a multi-view gated similarity calculation and an iterative optimization mechanism, and generating a patient-specific cell graph containing function semantic information; constructing a shared graph encoder and a classifier, and introducing a Wasserstein discriminator for adversarial training to realize cross-domain alignment of embedding distribution of the reference patient and the query patient; and finally predicting the type of the query patient cell by using the trained model. The application solves the problem of significantly improving the accuracy, robustness and biological interpretability of cross-patient cell annotation.

This application discloses an in vitro reconstructed vaginal mucosa model, its construction method, and its application. The construction method includes an improved culture medium: HVU medium is added to immortalized vaginal epithelial cell line VK2 / E6E7, which is then seeded into Transwell chambers and cultured. The HVU medium on the surface of the culture in the Transwell chambers is aspirated, and the Transwell chambers are raised to the air-liquid interface for air-liquid surface culture. The culture medium is then replaced with HVA medium to obtain the vaginal mucosa model. This method improves the stability of cells during in vitro culture and constructs an in vitro reconstructed vaginal mucosa model with a tissue structure highly similar to human vaginal mucosa (possessing a complete stratified squamous epithelial structure and normal intercellular connections), thereby enhancing the model's physiological functional simulation.

The application belongs to the technical field of cell engineering and cell differentiation regulation, and discloses a method for directionally inducing secretion function cells from iPSCs and application thereof. The method takes the initial state of iPSCs and the potential of secretion function as cores, and constructs a pretreatment-induction differentiation integrated system. By screening and passing, iPSC clones with specific adhesion characteristics and enzyme sensitivity are enriched, and an iPSC population with enhanced secretion function potential is obtained. In the differentiation initiation stage, a culture system containing a Rock inhibitor is used in combination with laminin modified culture surface to maintain the intercellular connection state and realize the synchronous response of the induction signal. Then, the directionally induced differentiation is carried out by adding induction factors in stages, and the obtained cells are screened and purified to prepare high-purity secretion function cells. The prepared cells can be widely applied to drug screening, cell therapy and disease model construction, and provide technical support for the large-scale preparation of clinical-grade secretion function cells.