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Mouse fat stem cell culture solution

A technology of adipose stem cells and culture medium, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of high cost and complex components of small factors, and achieve the effect of simple components and low cost

Inactive Publication Date: 2012-05-02
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As an important model animal, mice are widely used in basic research, but it has always been a problem to maintain long-term culture of mouse adipose stem cells in vitro. Although there are also reported culture mediums, in addition to basic components, additional The added small factors are complex, and relevant literature reports that the added small factors include epidermal growth factor (EGF), linoleic acid-bovine serum albumin, β-mercaptoethanol, insulin, transferrin, selenous acid, and platelet-derived factor (PDGF) and other components, and caused a high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] In this embodiment, the screening test was carried out on the mouse adipose stem cell culture medium according to the following steps.

[0015] 1. Under the same isolation conditions, the primary isolated cells were cultured with high-glucose medium, low-glucose medium and mouse embryonic stem cell medium respectively.

[0016] The recipes are as follows:

[0017] (1) High glucose medium (50ml)

[0018] Element

Stock solution concentration

working concentration

Dosage

fetal bovine serum

10％

5ml

Penicillin

10 5 IU / ml (1000×)

100IU / ml

50μl

streptomycin

10 5 μg / ml(1000×)

100μg / ml

50μl

DMEM (high sugar, 4500mg / L)

44.9ml

[0019] (2) Low-sugar medium (50ml)

[0020] Element

Stock solution concentration

working concentration

Dosage

fetal bovine serum

10％

5ml

Penicillin

10 5 IU / ml (1000×)

100IU / ml

50μl

...

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Abstract

The invention discloses a mouse fat stem cell culture solution. The culture solution consists of the following components: 20 percent of fetal calf serum, 100 IU / ml of penicillin, 100 mug / ml of streptomycin, 2 mmol / L of L-glutamine, 10 ng / ml of basic fibroblast growth factor, 50 mug / ml of vitamin C, and the balance of Dulbecco's modified eagle medium F12 (DMEM / F12). The culture solution has simple component and low cost, can effectively maintain in vitro long-term propagation of mouse fat stem cells, and can well maintain the biological properties of the fat stem cells.

Description

[technical field] [0001] The invention relates to a culture medium of stem cells, in particular to a culture medium of mouse adipose stem cells. [Background technique] [0002] Adipose stem cells have the potential of multi-directional differentiation, and can be directional differentiated into fat, osteoblast, cartilage, liver, cardiomyocytes, etc. in vitro. Because of the advantage of pain when taking material from the body, it has important significance in regenerative medicine. [0003] As an important model animal, mice are widely used in basic research, but it has always been a problem to maintain long-term culture of mouse adipose stem cells in vitro. Although there are also reported culture mediums, in addition to basic components, additional The added small factors are complex, and relevant literature reports that the added small factors include epidermal growth factor (EGF), linoleic acid-bovine serum albumin, β-mercaptoethanol, insulin, transferrin, selenous acid...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 魏超张运海张宇李运生章孝荣吴蓉花周娜汝刘星陈建文陶佳
Owner ANHUI AGRICULTURAL UNIVERSITY
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