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Cultivation method for improving phenolic acid components of stems and leaves of male plants of trichosanthes kirilowii Maxim

A cultivation method and a technology of phenolic acids, applied in the field of crop cultivation, can solve the problems that the stems and leaves of Trichosanthes quinquefolius can not be effectively utilized, and achieve the effects of delaying flowering time, increasing the content of phenolic acids, and increasing economic value

Inactive Publication Date: 2018-11-06
ANHUI ZANJIATIAN ECOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a cultivation method for improving the phenolic acid components in the stems and leaves of Trichosanthes male plants in view of the existing problem that the stems and leaves of Trichosanthes can not be effectively utilized

Method used

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  • Cultivation method for improving phenolic acid components of stems and leaves of male plants of trichosanthes kirilowii Maxim

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Experimental program
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Effect test

Embodiment 1

[0024] A cultivation method for increasing the phenolic acid components in stems and leaves of Trichosanthes mellifera male plants, comprising a stage of tissue seedling cultivation and a stage of transplanting culture, wherein the stage of tissue seedling cultivation includes the following contents:

[0025] (1.1) Select a well-growing biennial male Trichosanthes quinquefolium plant, take its branches, cut off the leaves and petioles, rinse with 1% saline for 5 minutes, and cut the branches into 1.6cm stems with axillary buds, the distance between axillary buds The distance from one end of the stem section growth direction is 0.6cm, and it is disinfected, and after completion, the two ends of the stem section are respectively cut off by 0.2cm to obtain the initial body of seedling cultivation;

[0026] (1.2) Cultivate the obtained primary seedlings in medium A for 13 days to obtain seedlings containing bud clusters. The culture temperature is 30°C. The light-dark cycle include...

Embodiment 2

[0038] A cultivation method for increasing the phenolic acid components in stems and leaves of Trichosanthes mellifera male plants, comprising a stage of tissue seedling cultivation and a stage of transplanting culture, wherein the stage of tissue seedling cultivation includes the following contents:

[0039] (1.1) Select a well-growing biennial male Trichosanthes quinquefolium plant, take its branches, cut off the leaves and petioles, rinse with 1% saline for 5 minutes, and cut the branches into 1.6cm stems with axillary buds, the distance between axillary buds The distance from one end of the stem section growth direction is 0.6cm, and it is disinfected, and after completion, the two ends of the stem section are respectively cut off by 0.2cm to obtain the initial body of seedling cultivation;

[0040] (1.2) Cultivate the obtained primary seedlings in medium A for 12 days to obtain seedlings containing bud clusters. The culture temperature is 32°C. The light-dark cycle includes ...

Embodiment 3

[0052] A cultivation method for increasing the phenolic acid components in stems and leaves of Trichosanthes mellifera male plants, comprising a stage of tissue seedling cultivation and a stage of transplanting culture, wherein the stage of tissue seedling cultivation includes the following contents:

[0053] (1.1) Select a well-growing biennial male Trichosanthes quinquefolium plant, take its branches, cut off the leaves and petioles, rinse with 1% saline for 5 minutes, and cut the branches into 1.6cm stems with axillary buds, the distance between axillary buds The distance from one end of the stem section growth direction is 0.6cm, and it is disinfected, and after completion, the two ends of the stem section are respectively cut off by 0.2cm to obtain the initial body of seedling cultivation;

[0054] (1.2) Cultivate the obtained primary seedlings in medium A for 14 days to obtain seedlings containing bud clusters. The culture temperature is 28°C. The light-dark cycle include...

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Abstract

The invention belongs to the technical field of crop cultivation, and particularly relates to a cultivation method for improving phenolic acid components of stems and leaves of male plants of trichosanthes kirilowii Maxim, and the cultivation method includes a tissue seedling stage and a transplanting cultivation stage. Compared with the prior art, the cultivation method has the following advantages: the tissue seedling stage of the cultivation method aims at the male plants of the trichosanthes kirilowii Maxim for culture of seedlings, the differentiation rate of seedling bodies containing clumpy buds is more than 82%, the average number of the buds is more than 15, the rooting rate of the seedlings of the trichosanthes kirilowii Maxim is about 75%, the average root length is about 1.8cm;the seedlings of the trichosanthes kirilowii Maxim are reasonably densely planted, a multi-layer shed frame is set up to increase the growth of stems and leaves. An inhibitor is sprayed in first tendays of June to delay flowering time, reduce flowering and promote the growth of the stems and the leaves, because the stems and the leaves are not cut off, due to a too large and too dense amount ofthe stems and the leaves in the later stage, flowering and fruit setting can be reduced, the chemical components in the trichosanthes kirilowii Maxim can be changed, the phenolic acid components in the stems and the leaves of the trichosanthes kirilowii Maxim are significantly increased, and economic value is effectively increased.

Description

technical field [0001] The invention belongs to the technical field of crop cultivation, and in particular relates to a cultivation method for increasing the phenolic acid components in stems and leaves of male Trichosanthes mellifera. Background technique [0002] Trichosanthes is a perennial herbaceous vine, its fruit, peel, and seeds can be used medicinally, and its chemical components are diverse, including volatile oils, sterols, terpenes, flavonoids, phenols, proteins, and other compounds. The research on the chemical constituents of the medicinal parts of Trichosanthes has gradually increased. Among them, the stems and leaves of Trichosanthes male plant contain phenolic acids, which play an important role in the treatment of gastric cancer, hypertension and other traditional Chinese medicine formulas. The development potential is huge, and the planting area is increasing year by year. In the prior art, there is no cultivation method aimed at improving the chemical com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G22/05A01G7/06A01N65/34A01N43/58A01N43/16A01N37/02A01N43/12A01N45/00A01N47/28A01P21/00
CPCA01H4/00A01G7/06A01G22/05A01H4/001A01N37/02A01N43/12A01N43/16A01N43/58A01N45/00A01N47/28A01N65/00A01N65/34
Inventor 王俊山吴坤段琳钰段林森段士平段钰
Owner ANHUI ZANJIATIAN ECOLOGICAL AGRI CO LTD
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