Methods for separating and identifying DNA (deoxyribonucleic acid) binding protein through DNA co-immunoprecipitation

A technology for binding proteins and DNA molecules, which is applied in the field of separation and identification of DNA binding proteins to achieve the effect of improving experimental efficiency, stability and repeatability
CN103739664AActive Publication Date: 2014-04-23CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
Publication Date
2014-04-23

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Abstract

The invention relates to methods for separating and identifying DNA (deoxyribonucleic acid) binding protein, in particular to methods for separating and identifying DNA binding protein through a DNA co-immunoprecipitation technology. The separating method comprises the steps of extracting nucleoprotein of a cell, mixing a target DNA molecule and nucleoprotein, adding a molecular entity capable of being bound with the target DNA molecule, separating to obtain a nucleoprotein ingredient bound with the target DNA molecule, further separating the obtained nucleoprotein ingredient, and obtaining the DNA binding protein. The identifying method further comprises the step of identifying the DNA binding protein obtained by separating. The methods have the characteristics of good stability and repeatability, and significantly improve the experiment efficiency of identifying the unknown DNA binding protein. The methods provide beneficial help for subjects such as molecular behavioristics, molecular oncology, cytobiology and biochemistry deeply researching into cytogene transcriptional control and genetic transcription.
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Description

technical field

[0001] The invention relates to a method for separating and identifying DNA binding proteins, in particular to a method for separating and identifying DNA binding proteins using DNA co-immunoprecipitation technology. Background technique

[0002] Biomedical research has evolved from the hypothesis of one gene and one protein to analyzing the functions of many genes or proteins at the omics level. Due to the complexity of various life activities of cells, the sequence information of the whole genome cannot explain the biological functions of cells, and the final product of genes is the ultimate executor of cell composition and function. In the process of cell life, the opening and closing of specific genes is a complex system engineering, and the transcriptional regulation of human genes has always been a hot research field for biologists. At present, chromatin immunoprecipitation (ChIP) is mainly used to study the interaction between known protein transcript...

Claims

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