Method for detecting DNA (deoxyribonucleic acid) binding protein

A technology of binding protein and detection method, which is applied in the field of detection of DNA binding protein, and can solve the problem of low sensitivity
CN103472236AActive Publication Date: 2013-12-25深圳柏垠生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
深圳柏垠生物科技有限公司
Publication Date
2013-12-25

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Abstract

The invention discloses a method for detecting DNA (deoxyribonucleic acid) binding protein. The method comprises the following steps: providing a sample solution of the DNA binding protein to be detected, and carrying out digestion reaction under the condition that Exo III exists, thus obtaining a digestive juice after complete reaction; adding the digestive juice in an amplification system, carrying out isothermal amplification reaction under the condition that a polymerase, an incision enzyme and an amplification template exist to obtain an amplification solution; mixing a gold nanoparticle solution with a solution containing a first detection probe and a second detection probe and then carrying out incubation to obtain a nano gold detection probe solution; mixing the amplification solution with the nano gold detection probe solution and then detecting a mixed solution to complete the detection for the DNA binding protein. The first detection probe and the second detection probe are combined with a target DNA in an amplification product so that gold nanoparticles of the detection probes gather to cause that the color of the mixed solution changes, thus the method is visual in phenomenon and high in sensitivity.
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Description

technical field

[0001] The invention relates to the field of gene detection, in particular to a detection method of DNA binding protein. Background technique

[0002] Simple and sensitive detection of transcription factors in human cells is of great significance for clinical diagnosis and drug screening. The current detection methods are all based on electrophoretic migration, immunochemical or fluorescence detection methods, which are directly detected by non-amplification, so the sensitivity is low. Moreover, these methods have radioactive contamination or require specific antibodies and fluorescently labeled probes, which greatly increase the cost and complexity of the experiment.

[0003] DNA-binding proteins play crucial roles in genome replication, gene transcription, cell division and DNA repair processes. Most of the DNA-binding proteins play the role of transcription factors, regulating cell development, differentiation and proliferation, so transcription factors ...

Claims

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