Method for culturing cord blood lymphocyte DC-CIK

A technology of DC-CIK and lymphocytes, which is applied in the direction of cell culture active agents, blood/immune system cells, tissue culture, etc., can solve the problem of CIK cell proliferation multiples and effective cell content, increased expression, and failure to take into account The effect of T regulatory cell content on tumor immune escape and other issues can be achieved to improve the activation efficiency and expansion efficiency, increase the expansion level, and increase the effect of killing activity
CN105969727APending Publication Date: 2016-09-28山东省齐鲁细胞治疗工程技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
山东省齐鲁细胞治疗工程技术有限公司
Publication Date
2016-09-28

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Abstract

The invention belongs to the technical field of immune cells in-vitro culture, and discloses a method for culturing cord blood lymphocyte DC-CIK. According to the invention, cord blood is taken as a processing object, and is subjected to separation of cord blood mononuclear cells, isolated culture of DC cell, induction culture of CIK cell, and co-culture of DC and CIK to prepare the DO-DIK cell, CTLA-4mAb and PD-1mAb are added, and the maturated DO-DIK cell is subjected to centrifugal collection to obtain the product. By using CD3mAb coating culture bottle, continuous stimulation is carried out at an early stage of culture, cytokine usage is reduced, effector cell population activation efficiency and amplification efficiency are increased, and the culture cost is reduced.
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Description

(1) Technical field

[0001] The invention belongs to the technical field of in vitro culture of immune cells, in particular to a method for culturing umbilical cord blood lymphocyte DC-CIK. (2) Background technology

[0002] Adoptive immunotherapy is to infuse sensitized lymphocytes (with specific immunity) or their products to patients with low cellular immunity (such as tumor patients) to improve the anti-tumor immunity of patients, so as to achieve the purpose of treatment and prevention of recurrence.

[0003] Dendritic cells (Dendritic cells) are the most powerful professional antigen-presenting cells (Antigen presenting cells, APCs) in the body. Mature DCs can effectively activate naive T cells, and are at the center of initiating, regulating, and maintaining immune responses. It can present antigens to T lymphocytes, thereby exerting the killing effect of T lymphocytes.

[0004] Cytokine-induced killer cells (CIK) is the combination of human peripheral blood or umbili...

Claims

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