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Identification of molecular diagnostic markers for endometriosis in blood lymphocytes

A technology for endometriosis and endometriosis, applied in the direction of microbial determination/testing, drug combination, biochemical equipment and methods, etc., can solve problems such as incurable and no specific laboratory tests

Inactive Publication Date: 2008-02-13
UNITED STATES OF AMERICA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite the urgent need for non-surgical methods of diagnosing endometriosis and non-invasive means for prognosis and treatment monitoring, there is still no specific laboratory test based on the identification of markers in blood (Falcone, T. and Mascha, E .2003 Fertil Steril 80: 886-888)
In addition, afflicted women who suffer from severe chronic pain, infertility, and emotional distress have few treatment options, none of which cure the disease
Finding candidate genes to establish endometriosis-specific test proves challenging

Method used

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  • Identification of molecular diagnostic markers for endometriosis in blood lymphocytes
  • Identification of molecular diagnostic markers for endometriosis in blood lymphocytes
  • Identification of molecular diagnostic markers for endometriosis in blood lymphocytes

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0100] research population

[0101] Study individuals (patients and controls) were recruited through referrals from partner obstetrics and gynecology clinics operating throughout Puerto Rico. The patient population studied included premenopausal women diagnosed with endometriosis during surgery by a specialist in obstetrics and gynecology, and included patients of all disease stages: severe (11), moderate (6), mild (3 ), min(1). Patient samples for microarray (N=6; age range 32-39 years; mean 35.5 years) and for real-time RT-PCR confirmation (N=15; age range 26-39 years; mean 31.2 years) are randomly selected from our nucleic acid library. Thirteen of 21 patients (62%) were not receiving any medication at the time the samples were taken. Those receiving medical treatment took GnRH agonists (6), oral contraceptives (1) and testosterone (1). Control groups (N=4 for microarray; N=15 for RT-PCR) were women who underwent laparoscopy or laparotomy for an unrelated gynecologic st...

Embodiment 2

[0108]We completed the analysis of data from 14 additional patients. We included a scatterplot of the raw data (group 1) to better represent individual variation in these markers (Fig. 3). Importantly, the scatter plot shows that low expression levels of LOXL1 are present in all patients tested so far.

[0109] Likewise, we included additional data from another subgroup of 14 patients (group 2), which confirmed the original findings. The figure below gives a summary of the results for the second group of patients studied. Figure 4 includes scatterplots showing the individual variation of each gene in Group 2. Figure 5 presents the mean fold expression results for patients in Group 1 (upper panel) and Group 2 (lower panel), while Figure 6 shows a comparison of the results for the two groups. Figure 7 graphs the data from the 39 patients tested to date.

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Abstract

The invention comprises a method of identifying or predicting the predisposition to endometriosis in a female subject comprising determining the level of gene expression of at least one differentially-expressed gene or protein or peptide of peripheral blood leukocytes in a sample of peripheral blood leukocytes or peripheral blood in a subject to provide a first value, determining the level of gene expression of the at least one differentially-expressed gene or protein or peptide of said leukocytes in a control or reference standard to provide a second value, and comparing whether there is a difference between the first value and second value.

Description

[0001] related application [0002] This application claims the benefit of US Provisional Application No. 60 / 654,331, filed February 18, 2005, which is hereby incorporated by reference in its entirety. field of invention [0003] Analysis of gene expression patterns in peripheral blood leukocytes of patients with endometriosis using genetic targets identified by DNA microarrays for use in a non-invasive diagnostic assay for the disease. Background of the invention [0004] Endometriosis is a fairly common gynecological disorder, affecting up to 10% of women of reproductive age (Mahmood, T.A. and Templeton, A. 1991 Hum Reprod 6:544-549). The pathogenesis of endometriosis remains unclear, and the mechanisms of establishment, progression, and migration of endometriotic lesions to extrapelvic locations are not well understood (Witz, C.A. et al. 2003 Hum Fertil 6 :34-40). Diagnosis of endometriosis is usually by visual inspection of the pelvis by laparoscopy (Attaran, M. et al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q2600/158C12Q1/6883C12Q2600/136A61P15/00C12Q1/6827
Inventor 伊达哈里兹·弗洛里斯斯皮罗·莫萨斯埃斯特尔·罗森布卢姆
Owner UNITED STATES OF AMERICA
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