Anti-idiotypic nano antibody based zearalenone green immunoassay method

A zearalenone and nanobody technology, applied in the field of molecular biology, can solve the problems of long production cycle, small antibody molecular weight, complicated preparation process, etc., and achieve the effects of good accuracy, high library capacity and good diversity
CN107602701AInactive Publication Date: 2018-01-19INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Publication Date
2018-01-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

Belonging to the field of molecular biology, the invention in particular relates to an anti-idiotypic nano antibody based zearalenone green immunoassay method. According to the invention, the zearalenone monoclonal antibody 2D3 is employed to immunize alpaca, and the leukocyte total RNA is extracted to construct a phage displayed nano antibody library; the 2D3 antibody is adopted as the target, four rounds of affinity enrichment panning are conducted to lower the coating concentration and competitive elution concentration round by round, and a positive phage zearalenone anti-idiotypic nano antibody with good specificity can be obtained. The invention employs the zearalenone anti-idiotypic nano antibody substitute zearalenone standard ELISA method to determine naturally polluted corn and feed samples, and performs comparison with the HPLC detection result, the two methods have good correlation, and R2 is 0.997, thus proving that the zearalenone anti-idiotypic nano antibody substitute standard method has accurate and reliable result and is an effective and feasible green immunoassay method.
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Description

technical field

[0001] The invention belongs to the field of molecular biology, and in particular relates to an anti-idiotype nanobody-based zearalenone green immunoassay method. Background technique

[0002] Zearalenone (ZEN) is a mycotoxin produced by Fusarium fungus, which mainly contaminates corn, wheat, sorghum and other cereal crops. It has reproductive toxicity, immunotoxicity and potential carcinogenicity. The toxin can enter the human and animal body by contaminating grain and its products or meat and milk with ZEN residue, posing a serious threat to the development of animal husbandry and human health. In view of the widespread contamination of zearalenone in agricultural products and foods and the exposure level of residents' diets, most countries have established the maximum allowable amount of ZEN in grains and products.

[0003] Currently, methods for ZEN detection include thin layer chromatography, high performance liquid chromatography, immunoassay and elect...

Claims

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