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Nano particle composition and anti-tumor application thereof

A nanoparticle and composition technology, which is applied in the direction of antineoplastic drugs, non-active ingredients of polymer compounds, drug combinations, etc., can solve the problems of increasing treatment costs and affecting the effect of treatment, so as to prolong life or survival time, prevent Metastasis or recurrence and the effect of reducing mortality

Inactive Publication Date: 2016-03-23
XUZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the gene therapy programs of these viral vectors still have problems such as biosafety caused by genome integration and the body's immune response to viral vectors.
However, in order to achieve the therapeutic effect of polypeptide drugs, continuous high-dose repeated administration is required, which will seriously affect the therapeutic effect and increase the cost of treatment.

Method used

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  • Nano particle composition and anti-tumor application thereof
  • Nano particle composition and anti-tumor application thereof
  • Nano particle composition and anti-tumor application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Preparation of PEI-CyD-PEG-FA

[0028] A previous patent synthesized a polycationic carrier (named H1) that uses cyclodextrin (CyD) as a cross-linked backbone, connects low-molecular-weight polyethyleneimine (PEI), and couples a small-molecule drug folic acid with tumor targeting specificity. ). H1 has been proved to be able to encapsulate and concentrate the plasmid expressing the target gene into a nanoparticle with a size of about 100 nanometers in a liquid environment. In a variety of tumor cell lines, this nanoparticle showed low toxicity, high efficiency and target-specific gene delivery ability. Treatment of cancer, particularly malignant melanoma, using H1-transduced nanoparticles expressing anti-tumor cytokines.

[0029] Polyethylene glycol (PEG) is a hydrophilic polymer, which can improve the hydrophilicity of the surface of colloidal particles and the stability of ionic solutions, thereby reducing the complement conditioning and the removal of the reticu...

Embodiment

[0048] Example: 48 Balb / c mice According to the method described in the preliminary experiment, an orthotopic liver cancer mouse experimental animal model inoculated with ML-1 cells was established. Seven days after the operation, the mice were randomly divided into 5 groups, 12 mice in each group, and were given intraperitoneal injection of PCPFA / pLuc (DNA 100ug) and PCPFA / pLuc (DNA 100ug) respectively. One month after treatment, 4 mice were randomly selected from each group to extract tumor tissue, weighed and collected for subsequent mechanism studies; the remaining mice continued to be observed for survival studies.

[0049] The results showed that: 30 days after treatment, the median weight of tumor tissue in the PCPFA / pLuc treatment group was 520 mg; in the PCPFA / pHGFK1 treatment group, the median weight of tumor tissue was 180 mg. This indicated that PCPFA / pHGFK1 treatment significantly inhibited the growth of liver orthotopic inoculated tumors. attached Figure 6 It ...

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Abstract

The invention relates to a nano particle composition and anti-tumor application thereof. The nano particle composition is a nano particle formed by assembling a polymer carrier and plasmid for expressing a therapeutic gene; the polymer carrier is a cyclodextrin-polyethyleneimine-polyethylene glycol-folic acid quaternary assembly-type polycation carrier, and the plasmid for expressing the therapeutic gene is eukaryotic expression plasmid containing an HGFK1 protein DNA sequence for coding a hepatocyte growth factor. Compared with the prior art, the nano particle has the advantages of being efficient and tumor targeted; the nano particle can be automatically assembled in an aqueous solution through electrostatic interaction, and the particle size of the nano particle and the stability in a salt solution has an important effect on the function of the nano particle; it is verified that the nano particle composition can prevent transfer or relapse of a cancer and alleviate or relieve or control or improve or cure a primary cancer or a metastatic cancer or other related symptoms and can also prolong the life or survival time of a cancer patient and reduce the mortality.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and in particular relates to a nanoparticle composition and its antitumor application. Background technique [0002] Malignant tumor (cancer) is a major human disease, and it is of great significance to strengthen the research and development of new anti-tumor drugs. There are more than 22 million patients with malignant tumors in the world, 10.1 million new patients are diagnosed every year, and more than 6.2 million patients die of cancer every year. There are at least 3 to 4 million malignant tumor patients in my country, with more than 1.6 million newly diagnosed patients and more than 1.3 million annual deaths. With the rapid development of my country's economy and society, the extension of average life expectancy, and the changes in dietary structure and environmental factors, the incidence and mortality of tumors have shown a rapid upward trend, and the spectrum of diseases h...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K9/14A61K47/40A61P35/00A61P35/04
Inventor 姚宏林李家宓孔祥复郑骏年沈赞
Owner XUZHOU MEDICAL COLLEGE
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