Magnetic fluorescent double-function nanoion probe and preparation method thereof

A magnetic nano, magnetic nano particle technology, applied in nanotechnology, nanotechnology, nano optics, etc., can solve the problems of high price, limited fluorescence emission wavelength range, etc., and achieve the effect of simple process, easy operation and avoiding leakage

Inactive Publication Date: 2015-07-01
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method requires the addition of expensive rare earth elements, and due to the l...

Method used

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  • Magnetic fluorescent double-function nanoion probe and preparation method thereof
  • Magnetic fluorescent double-function nanoion probe and preparation method thereof
  • Magnetic fluorescent double-function nanoion probe and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] 1.1 ZnSe quantum dots were prepared by the aqueous phase method. Under nitrogen protection, 0.01g NaBH 4 Dissolve 0.0061g of Se powder in 2 mL of distilled water, heat to 40°C, and obtain NaHSe solution after completely dissolving; take 0.0439 g of Zn(Ac) 2 Dissolve in 20 mL of distilled water, add 0.0737 g of reduced glutathione after it is completely dissolved, and adjust the pH to 11.5 with 1 mol / L NaOH solution to obtain a Zn precursor solution; transfer the Zn precursor into a three-necked flask NaHSe solution was quickly injected under the protection of nitrogen, and the oil bath was refluxed at 100°C. After magnetic stirring for 1 h, it was taken out and quickly cooled to room temperature in the refrigerator to obtain a colorless and transparent solution, which was the prepared ZnSe QDs solution.

[0039] 1.2 Preparation of Fe by hydrothermal method 3 o 4 nanoparticles. Weigh 3 g FeCl 3 Dissolve in 80 mL of ethylene glycol, stir and ultrasonically dissolve, ...

Embodiment 2

[0044] 2.1 Preparation of ZnSeZnS quantum dot solution. Firstly, ZnSe quantum dots were prepared by chemical co-precipitation method, and the preparation method was as described in the above-mentioned Example 1. Take 15 mL of prepared ZnSe quantum dots, in which the concentration of ZnSe quantum dots is 2.7×10 -3 mol / L, add 0.0138 g Zn(Ac) 2 , 0.0277 g reduced glutathione and 0.01 g thiourea, adjust the pH value to 10.5 with the prepared NaOH solution with a concentration of 1 mol / L, under magnetic stirring, reflux the oil bath at 100°C, and react for 2 h after magnetic stirring Put it into the refrigerator and quickly cool it to room temperature to obtain light yellow water-soluble ZnSeZnS quantum dots with high luminous efficiency.

[0045] 2.2 Preparation of Fe by hydrothermal method 3 o 4 nanoparticles. Take 2.78 g FeSO 4 ·7H 2 O, 4.32 g FeCl 3 ·6H 2 Dissolve O in 30 mL of distilled water, stir and dissolve with magnetic force, then add 30 mL of ethylene glycol, ...

Embodiment 3

[0050] 3.1 Preparation of CdTe quantum dots in aqueous phase. Under nitrogen protection, 0.0945g NaBH 4 Dissolve 0.0063g Te powder in 5 mL distilled water, heat to 40°C, and obtain NaHTe solution after completely dissolving; take 0.293 g Cd(Ac) 2 Dissolve in 100 mL of distilled water, add 2.0 mmol after it is completely dissolved, adjust the pH=11 with 1mol / L NaOH solution, and obtain the precursor solution of Cd; transfer the precursor of Cd into a three-neck flask, Quickly inject NaHTe solution, reflux in an oil bath at 100°C, magnetically stir for 4 hours, take it out and put it in the refrigerator to cool rapidly to room temperature to obtain a colorless and transparent solution, which is the prepared CdTe QDs solution.

[0051] 3.2 Preparation of magnetic nanoparticles. Adopting the patent publication number to prepare Fe 3 o 4 / CoO core-shell magnetic nanoparticles.

[0052]3.3 Preparation of chitosan-modified magnetic nanocomposites (CMCH-MNPs) by chemical covalent...

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Abstract

The invention belongs to the field of preparing a nanomaterial, and particularly relates to a magnetic fluorescent double-function nanoion probe and a preparation method thereof. The composite microparticles have the fluorescent performance of quantum dots and the magnetism performance of magnetic nanoparticles, and can be used as target positioning in biological body and biological fluorescent imaging aspects. The invention provides a preparation method of the magnetic fluorescent double-function nanomaterial, and the magnetic fluorescent double-function nanomaterial is obtained by taking chitosan-modified magnetic nanoparticles as a core and connecting water-soluble quantum dots through adopting an ionic crosslinked method, the fluorescent quantum dots are distributed on the surfaces of the magnetic nanoparticles with the particle size of 10-200nm, and the particle size of the quantum dots is 1.5-10nm. The preparation method is mild in reaction conditions, the operation method is simple, the prepared composite nanoparticles have good luminescence performance and magnetic performance, and have wide application prospects in the fields of biomarking, fluorescent immunoassay, bioseparation, protein DNA enrichment and separation, the preparation of drug carrying system, and target imaging and the like.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation, and in particular relates to a magnetic fluorescent dual-functional nano-ion probe and a preparation method thereof. The composite particles have both the fluorescent properties of quantum dots and the magnetic properties of magnetic nanoparticles, and can be used as targets in living organisms. orientation and bioluminescence imaging. Background technique [0002] With the further development of nanotechnology research, people's requirements for nanomaterials have changed from monodispersity and shape control to the construction of multi-component composite nanoparticles. Combining a variety of different materials through methods such as surface epitaxial growth has become a new hotspot in nanoscience and technology research. Compared with single-component nanoparticles, multifunctional nanomaterials have a variety of excellent properties at the same time, which better solves the problem...

Claims

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Application Information

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IPC IPC(8): C09K11/88B82Y30/00B82Y20/00B82Y25/00G01N21/64
CPCB82Y20/00B82Y25/00B82Y30/00B82Y40/00C09K11/025C09K11/88C09K11/883G01N21/6428G01N21/643H01F1/0018
Inventor 刘福田丁永玲孙康宁
Owner UNIV OF JINAN
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