Transgenically produced non-secreted proteins

a technology of transgenes and proteins, applied in the field of protein production and secretion, can solve the problems of difficult or expensive production of/or in the required quantities using conventional methods, and the difficulty of traditional bacteria or yeast systems to produce many complex proteins in a functional form, etc., to achieve the effect of improving the expression level, increasing the yield of desired proteins, and stabilizing the rna of the expression sequen
US20060179493A1Inactive Publication Date: 2006-08-10GTC BIOTHERAPEUTICS INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
GTC BIOTHERAPEUTICS INC
Publication Date
2006-08-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method of making and secreting a non-secreted protein. The method includes expressing the protein from a nucleic acid construct which includes: (a) a mammary epithelial specific promoter; (b) a milk protein specific signal sequence which can direct the secretion of a protein; (c) optionally, a sequence which encodes a sufficient portion of the amino terminal coding region of a secreted protein to allow secretion in the milk of a transgenic mammal, of the non-secreted protein; and (d) a sequence which encodes a non-secreted protein, wherein elements (a), (b), optionally (c), and (d) are preferably operatively linked in the order recited. Both glutamic acid decarboxylase (GAD) and myelin basic protein (MBP), which are cytoplasmic proteins, have been produced by the methods of the present invention. The invention also provides methods for treating diabetes and multiple sclerosis using proteins produced by the methods of the present invention.
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Description

[0001] This application claims the benefit of a previously filed Provisional Application No. 60 / 038,998, filed Feb. 25, 1997, which is hereby incorporated by reference.FIELD OF THE INVENTION

[0002] This invention relates to the production and secretion of proteins which are not ordinarily secreted. BACKGROUND OF THE INVENTION

[0003] A growing number of recombinant proteins are being developed for therapeutic and diagnostic applications. However, many of these proteins may be difficult or expensive to produce in a functional form and / or in the required quantities using conventional methods. Conventional methods involve inserting the gene responsible for the production of a particular protein into host cells such as bacteria, yeast, or mammalian cells, e.g., COS cells, and then growing the cells in culture media. The cultured cells then synthesize the desired protein. Traditional bacteria or yeast systems may be unable to produce many complex proteins in a functional form. While mamma...

Claims

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