The invention belongs to the technical field of polypeptide chemistry and immunology and discloses a myelin basic protein antibody detection kit. According to the kit, a double-antigen sandwich methodis adopted to detect the content of a human MBP antibody; a coated plate, a calibration product, an MBP conjugated antigen, an enzyme conjugate, a quality control product, a color developing agent A,a color developing agent B, a stop solution and a concentrated washing solution are arranged in the kit; the coated plate is coated with an MBP coating antigen; an X fragment in the epitope peptide of the human MBP antigen is coupled with a carrier protein, so that the MBP coating antigen can be prepared; a Y fragment in the epitope peptide of the human MBP antigen is coupled with a carrier protein, so that the MBP conjugated antigen can be prepared; and the MBP conjugated antigen is labeled with biotins. With the method adopted, defects in the prior art are eliminated; the content of the human MBP antibody is quantitatively detected through the double-antigen sandwich method. The myelin basic protein antibody detection kit has the advantages of simple, convenient and rapid operation, high detection accuracy, high precision, high specificity, high sensitivity, and high stability.