35 results about "Nonhuman primate" patented technology

The utility model discloses a non-contact drinking water system for non-human primates, which comprises a cage, a main line water supply unit and a branch line water supply unit, the main line water supply unit comprises a constant-pressure water source and a drinking water main pipeline, and the drinking water main pipeline is communicated with the interiors of the constant-pressure water source and the branch line water supply unit; one end of the branch water supply unit is communicated with the drinking water main pipeline, and the other end of the branch water supply unit is communicated with the interior of the cage; a water inlet hole is formed in one side of the cage, and the water inlet hole is communicated with the interior of the branch water supply unit. The non-human primate breeding device can well adapt to the breeding scale, funds and breeding technology of the non-human primates, is simple in structure, low in cost, energy-saving and environment-friendly, and can avoid breeding water supply pollution so as to be beneficial to the physical health of the non-human primates.

Provided herein are TfR binding VHH polypeptides which bind to human and non-human primate TfR, conjugates thereof, and pharmaceutical compositions comprising these. Provided are also methods of use comprising administering TfR binding VHH polypeptides, conjugates thereof or pharmaceutical compositions thereof to deliver agents, including therapeutic agents, to treat a disease or disorder.

The invention relates to a touch fluid neuron specific tracing imaging agent, a preparation method and application, and belongs to the technical field of nuclear medicine imaging, the tracing imaging agent is composed of a nerve tracer agent horseradish peroxidase (HRP), zirconium-89 isotope chelated by deferoxamine and a pharmaceutically acceptable carrier, the effective tracking window of the tracer agent is prolonged to 120 hours, and the effective tracking window of the tracer agent is prolonged to 120 hours. The multi-stage synaptic transfer period of the fluid touching neuron can be completely covered; the spatial resolution is improved by 5-8 times compared with that of traditional FDG-PET, and a fluid touching neuron cluster can be accurately presented; the in-vitro serum stability is excellent, the radiochemical purity is still larger than or equal to 80.5% within 120 hours, PET / MRI bimodal imaging can be adapted, drug administration is conducted through lateral ventricle minimally invasive injection, the probe is suitable for various animal models such as mice, rats and non-human primates, in-vivo dynamic tracing of sympathetic fluid neuron trans-synapses can be achieved on the premise that no tissue damage exists, and the probe has a good application prospect. And a key technical tool is provided for neural circuit analysis, neuroscience basic research and preclinical research of neurodegenerative diseases.

The invention relates to a barcoding method to assess transduction efficiency of AAV vectors, in particular in vivo in rodent and non-human primate.

The invention discloses a sperm genome methylation detection method for safety evaluation of biological breeding crops by using primates and application, and relates to the technical field of safety evaluation of crops, the sperm genome methylation detection method comprises the following steps: dividing non-human primates into three groups, collecting sperms after long-term feeding, and extracting DNA (Deoxyribose Nucleic Acid); carrying out whole genome sequencing and quality control after bisulfite treatment; the epigenetic safety of crops is comprehensively evaluated by analyzing the methylation level of a whole genome and a functional region and functional enrichment of a differential methylation region and related genes thereof; according to the sperm genome methylation detection method for evaluating the safety of the biologically bred crops by utilizing the primates and the application, by utilizing a high-resolution WGBS technology, subtle epigenetic changes which are difficult to find by traditional toxicology can be detected, and the sperm genome methylation detection method has important significance in cross-generation reproduction effect evaluation, and has a wide application prospect. A food safety evaluation system can be perfected, and a more scientific and reliable safety interpretation basis can be established.

The invention provides a puncture device for a pharmaceutical injection test, and relates to the technical field of medical experiments, the puncture device comprises a base mechanism, the base mechanism is fixed on a forearm of a non-human primate experiment body through a first medical adhesive tape and a second medical adhesive tape, and the base mechanism is fixedly provided with an injection tube through a locking mechanism; a middle connecting pipe is installed at one end of the injection pipe, a separation non-return hose is inserted into the end, away from the injection pipe, of the middle connecting pipe, and a needle mechanism is fixedly installed at the end, away from the middle connecting pipe, of the separation non-return hose. Through the cooperation of the base mechanism, the first medical adhesive tape, the second medical adhesive tape and the silica gel pad, comfortable and stable attachment of the device to the forearm of an experimental body is achieved, pulling caused by animal movement is effectively resisted, and through rigid locking of a threaded inclined plate and a fastening nut of the locking mechanism to an injection tube clamping convex ball, the clamping convex ball can be locked more firmly. And the reliability of connection between the infusion tube and the middle connecting tube is further ensured.

The invention relates to the technical field of non-human primate cognitive testing devices, in particular to a non-human primate-based behavioral training and testing device and method. The device comprises a fixing frame, a screen unit, a grabbing rod reward unit, a sensing unit, a transition cage and a controller. Wherein the screen unit comprises a screen and a screen clamping groove, and the screen is connected with one side of the fixing frame through the screen clamping groove; the grabbing rod reward unit comprises a grabbing rod frame, a grabbing rod, a liquid conveying hose, a suction nozzle hole, a peristaltic pump and a reward liquid carrier, one side of the grabbing rod frame is fixedly connected with the other side of the fixing frame, and the grabbing rod is connected with the grabbing rod frame; the sensing unit comprises an infrared sensor unit and a camera shooting unit; the transition cage comprises a movable plate and a cage body, and the movable plate is movably connected with the cage body; the controller is in communication connection with the screen, the sensing unit and the peristaltic pump. The test efficiency can be improved, the test error is reduced, the stress response is reduced, and the experiment reliability and flexibility are improved.

This application relates to a modular non-human primate rearing cage, belonging to the technical field of animal husbandry. It includes a cage frame, a resource supply system, a hygiene and cleaning system, an activity and enrichment system, and a connection and management system. The cage frame includes a cage base, a cage top, and cage side frames. The resource supply system includes a food supply device and a water supply device detachably installed on the cage frame. The connection and management system includes a connecting channel on the cage side frames and a connecting cage door movably installed on the cage side frames for changing the opening and closing state of the connecting channel. The activity and enrichment system includes a lookout component installed on the cage side frames, a climbing component located between the lookout component and the cage base, and multiple monitoring units installed on the cage top frame. This application can improve the husbandry and management efficiency of non-human primates.

A method and system are disclosed for differentiating non-human primate induced pluripotent stem cells (NHP iPSCs) into hepatocyte-like cells through a defined, multi-stage protocol. The method includes sequentially exposing NHP iPSCs to six distinct cell differentiation media over an approximate eighteen-day period. Each medium comprises defined combinations of growth factors, small molecules, and inhibitors that direct progressive lineage specification through anterior primitive streak, definitive endoderm, posterior foregut, liver bud progenitors, hepatoblasts, and hepatocyte-like cells. Concentration ranges and exposure durations are provided for each stage, ensuring reproducibility and reducing heterogeneity across cell populations. In some embodiments, treatment with a transforming growth factor beta (TGF-β ) receptor I inhibitor during early hepatoblast specification enhances lineage fidelity. The resulting hepatocyte-like cells are validated by immunostaining and western blotting for hepatic markers. Applications include preclinical drug metabolism, hepatotoxicity assessment, and in vitro disease modeling using non-human primate–derived systems.

The invention provides a targeting reagent for preparing fragile X syndrome animals and application of the targeting reagent. The invention discloses a target which is matched with non-human primates and is suitable for gene modification, namely a third exon and / or a fourth exon of an FMR1 gene, a target reagent is prepared by targeting the target, and a fragile X syndrome animal model with typical disease symptoms can be obtained by implementing down-regulation.

This invention discloses a neural microprobe for bimodal functional localization and precise electrical stimulation of deep brain nuclei in non-human primates. It comprises a basal layer, a conductive metal layer, and an insulating layer. The conductive layer includes a set of detection sites and stimulation sites. This invention employs a dry etching process on the back of the silicon basal layer using two insulating layers with opposite stresses, balancing the stress differences between the different materials. This overcomes the technological challenges of extending silicon microprobes and solves the problems of probe bending and low mechanical stress caused by the large aspect ratio of silicon probes in non-human primates. The detection sites acquire bimodal signals using modified nanomaterials and polymer anti-interference ion-exchange membranes as localization markers for functional localization. The detection sites are arranged between the positive and negative electrodes of the electrical stimulation, controlling the stimulation range to be near the detection sites, thus achieving precise electrical stimulation. This invention provides a new research method and means for deep brain stimulation therapy of diseases such as Parkinson's disease.

The invention relates to the field of medicines, in particular to a construction method of an Alzheimer's disease non-human primate model. A small dose of thiamine antagonist pyrithiamine is given to the chronic abdominal cavity to induce chronic thiamine metabolic disorder, so that the non-human primate marmoset Alzheimer disease model capable of reflecting the Alzheimer disease course is established, and the method has the advantages of simplicity in operation, small animal injury and the like; therefore, the method is beneficial to further basic clinical transformation research and the like, and has more important scientific significance and economic value.

The feeding barrel for the non-human primates comprises a barrel body and a barrel cover detachably installed on the barrel body, the barrel body is in a barrel shape with the top open, and the side wall of the barrel body is hollowed out so that the feeding position can be observed conveniently; the barrel cover is detachably hung on the top wall of the rearing cage, and the barrel body and the barrel cover are made of food-grade hard materials. According to the feeding barrel for the non-human primates, food is placed in the feeding barrel, so that the food is effectively prevented from being contaminated by dirt in a rearing cage; the hand and the arm of a non-human primate extend into the feeding barrel from the feeding hole for feeding, and meanwhile, the position of food is observed through the hollow structure, so that foraging is facilitated, the fun of exploring and finding in the foraging process of the non-human primate is increased, and animal welfare is increased; the barrel body and the barrel cover are preferably made of food-grade hard materials, can be effectively prevented from being damaged by non-human animals, are convenient to clean and meet the strict requirements of industries such as medicine research and development on sanitary conditions.

A method for differentiating non-human primate iPSCs into neurons may include providing a modified vector containing a piggyBac vector backbone, the modified vector expressing differentiation factor genes; treating the non-human primate iPSCs in a culture medium with the modified vector; replacing the culture medium with a second culture medium comprising BDNF, NT-3, and laminin; adding a culture medium supplemented with L-glutamine or an L-glutamine substitute, BDNF, and NT3; and generating neuronal lineage cells.

A method of differentiating nonhuman primate induced pluripotent stem cells (iPSCs) to oligodendrocytes is provided. The method may include providing a modified vector comprising a piggyBac vector backbone that expresses one or more differentiation factor genes, including SOX10, OLIG2, and NKX6-2. The piggyBac vector backbone may comprise one or more terminal inverted repeats and one or more transposase recognition sites configured to interact with a piggyBac transposase to mediate genomic integration at TTAA or noncanonical target sequences. The vector may further include a selectable marker and a promoter for regulated expression. The modified vector may be introduced into nonhuman primate iPSCs, for example from Macaca fascicularis, by electroporation and used in a feeder-free differentiation workflow. The resulting differentiated cells may be identified by expression of oligodendrocyte-associated markers including O4, O1, PDGFRA, MOG, GALC, CNPase, and myelin basic protein (MBP).

Disclosed are an antibody targeting the monkeypox virus, an antigen-binding fragment thereof, and a use thereof. The antibody or the antigen-binding fragment thereof comprises a heavy chain variable region and a light chain variable region. The antibody or the antigen-binding fragment thereof of the present invention targets A35R of humans and non-human primates.

The application belongs to the field of animal research, and discloses a non-human primate cooperation experiment method and device, which comprises the following steps: step one, using open source single-chip microcomputer code editing software to write required cooperation logic code and burning the code into a circuit board; step two, starting the animal cooperation experiment device; step three, an animal operating a pressing part; step four, judging the triggering condition of the pressing part, if the condition meets the cooperation logic, the cooperation experiment device outputs food, otherwise, the cooperation experiment device outputs a punishing content; and step five, resetting the animal cooperation experiment device; when multiple non-human primates complete cooperation, the application uses food as a reward to encourage cooperation among animals, so that the cooperation mode among non-human primates can be studied, the food stored in the food bin can be supplemented into the temporary storage cavity after the reward, so that the temporary storage cavity always has food, and the device has a small volume and can be installed outside a monkey cage for use at any time.

The invention belongs to the technical field of regenerative medicine, and discloses an organoid culture method derived from neural stem cells in subventricular regions of human and non-human primates. By optimizing a culture medium formula and regulating and controlling transcription factors, the organoid is successfully induced to be differentiated into the neuron precursor for expressing the CRABP1. The cell expresses TAC3, the limitation of the prior art in simulation of special neuropedigree and complex brain structure of primates is overcome, and a breakthrough in-vitro model is provided for research on neurodevelopment and nervous system diseases and drug development. The established system not only can provide a new tool for researching the development law and disease mechanism of the primate nervous system, but also can provide a reliable experimental platform and a transplantable cell source for developing targeted drugs, constructing advanced disease models and developing cell replacement therapy.

The application discloses a non-human primate cage door locking device, which comprises a cage door, a T-shaped bolt lock and a locking structure. The cage door is provided with a plurality of cylindrical sleeves. The T-shaped bolt lock comprises a lock core part and a handle part. The lock core part is a horizontal circular rod in the axial direction, and the handle part is a vertical circular rod in the axial direction. The top end of the handle part is welded at the midpoint of the lock core part. The two ends of the lock core part are respectively inserted into the cylindrical sleeves. The T-shaped bolt lock can move left and right relative to the cage door. A through hole is formed in the T-shaped bolt lock. One end of the locking structure penetrates through the through hole and is detachably mounted on the T-shaped bolt lock. The non-human primate cage door locking device has the advantages of simple structure, easy operation, low cost, easy manufacturing, difficult learning of the opening and closing mode by monkeys, prevention of the monkeys from opening and closing the cage door by themselves, great improvement of the working efficiency of non-human primate cage production personnel and solution to the difficult problems in the intensive feeding of non-human primates.

The invention relates to the field of medicines, in particular to a construction method of an Alzheimer's disease non-human primate model. A small dose of thiamine antagonist pyrithiamine is given to the chronic abdominal cavity to induce chronic thiamine metabolic disorder, so that the non-human primate marmoset Alzheimer disease model capable of reflecting the Alzheimer disease course is established, and the method has the advantages of simplicity in operation, small animal injury and the like; therefore, the method is beneficial to further basic clinical transformation research and the like, and has more important scientific significance and economic value.

This invention discloses a non-human primate visual-spatial working memory testing cage. This invention uses a non-human primate visual-spatial working memory testing cage to measure the learning, discrimination, and reversal abilities, or cognitive and memory abilities, of non-human primates, providing a more comprehensive evaluation of changes in animal brain function and behavior. It can provide a model evaluation tool for central nervous system diseases such as Alzheimer's disease and stroke, and offer new methods for disease research and new drug development.

Provided herein are TfR binding VHH polypeptides which bind to human and non-human primate TfR, conjugates thereof, and pharmaceutical compositions comprising these. Provided are also methods of use comprising administering TfR binding VHH polypeptides, conjugates thereof or pharmaceutical compositions thereof to deliver agents, including therapeutic agents, to treat a disease or disorder.

The utility model relates to the technical field of animal experiments, in particular to a small non-human primate fixing plate which comprises a plate body and a plurality of bandage structures, and the plate body is provided with a plurality of through hole structures for the bandage structures to penetrate through. The perforated structures are at least arranged in the first area, the second area and the third area of the plate body, and at least two perforated structures which are arranged left and right and have a preset interval are arranged in each area. The fixing device is free of metal materials, the bandage structure and the plate body are simple and convenient to connect, the length between the two bandages is flexible to adjust, the fixing device has better adaptive capacity for different animals, rapid adjustment and fixing can be achieved during fixing, meanwhile, the trunk or the four limbs cannot be pressed, and the fixing device is convenient to use. And the use of the experimental animal in the medical, examination or transportation process can be met, and meanwhile, the advantages of safety and comfort are achieved.

The invention discloses a kit for detecting non-human primate tuberculosis and application thereof, and relates to the technical field of biology, the kit comprises a specific primer probe combination, a whole blood stimulant, a one-step RT-qPCR reaction solution and a nucleic acid extraction reagent; through the specific primer probe combination designed according to the characteristics of the non-human primate CXCL10 gene and the internal reference ACTB gene, cross-species universal detection can be achieved, reagents do not need to be independently designed according to different species, the application range is widened, the two probes are used for marking different fluorophores and corresponding quenching groups respectively, and the detection sensitivity is high. In the amplification process, double-channel synchronous detection can be realized, signal interference is avoided, the specificity and stability of detection are guaranteed, a whole blood stimulation process is matched with a one-step RT-qPCR reaction system, the operation steps are simplified, the loss and error in the sample treatment process are reduced, meanwhile, the whole detection period is shortened, the actual requirement of rapid screening is met, and the detection efficiency is improved. And a high-quality detection basis is provided for subsequent result interpretation.

Methods are provided for isolating and expanding gastrointestinal (GI) tract stem cells from nonhuman primates (NHP)s. The method includes recovering a portion of GI tissue from a NHP, enzymatically digesting the tissue in a digestion medium comprising a tissue dissociation enzyme, and isolating cells through filtration and centrifugation. The isolated cells are cultured in a two-dimensional or three-dimensional (3D) culture system using an expansion medium comprising a basal formulation supplemented with one or more growth factors, vitamins, amino acids, and pathway modulators. In some embodiments, the cells are suspended in a basement membrane extract and plated as a 3D structure for organoid formation. The resulting stem cells or organoids may be analyzed using microscopy, flow cytometry, or transcriptomic profiling, and may be used for preclinical drug testing, disease modeling, or toxicity screening. The methods reduce reliance on live animal testing and improve translational relevance of in vitro gastrointestinal models.

The application belongs to the technical field of biological medicine, and particularly discloses a construction method of a glaucoma non-human primate model, which comprises the following steps: injecting emulsified silicone oil into the anterior chamber of a non-human primate; and detecting after 7-8 weeks, i.e. obtaining the glaucoma non-human primate model. Compared with a traditional model, the animal model of the application does not need to repeatedly use expensive modeling instruments, nor does it need to repeatedly damage the animals by modeling multiple times. The reagent used is low in cost and easy to obtain, the modeling time is short, and the animal model can greatly save manpower, material resources and time cost, and reduce the overall preclinical development cost of new drugs.

The present invention relates to a method for producing a nonhuman primate animal model of cerebral infarction, comprising administering endothelin to basal ganglia and thalamic region of a nonhuman primate, and thereby inducing basal ganglia damage, thalamus damage, and internal capsule damage; and a pharmaceutical composition for the treatment of cerebral infarction at a subacute to chronic stage, penetrating branch infarction, or cerebral infarction having brain damage in a penetrating branch territory, comprising a NeuroD1 protein or a polynucleotide encoding the NeuroD1 protein.

The invention discloses application of a reagent for detecting abundance of intestinal flora markers in preparation of a non-human primate chronic enteritis in-vitro diagnosis product. According to the invention, cynomolgus monkeys are used as non-human primates, metagenome sequencing is carried out on bacterial components in feces of healthy individuals and chronic enteritis infected persons, the abundance of bacteria is obtained, 17 specific species of florae are found through screening operation as microbial markers, and a chronic enteritis risk prediction model is constructed. Experiments prove that the chronic enteritis risk prediction model is high in prediction sensitivity and good in specificity, chronic enteritis patients and healthy individuals can be effectively distinguished in multiple samples, and the accuracy is 97% or above, so that the flora can be used as a chronic enteritis detection marker for detecting chronic enteritis. A reagent for detecting the abundance of the intestinal flora marker can be used for preparing a non-human primate chronic enteritis in-vitro diagnosis product, so that a foundation is laid for research on non-human primate chronic enteritis diseases.

A method of differentiating nonhuman primate induced pluripotent stem cells (iPSCs) into astrocytes may include providing a modified vector comprising a piggyBac vector backbone configured to express SOX9 and NFIB. The piggyBac vector backbone may include terminal inverted repeats and transposase recognition sites that support stable genomic integration of the inserted differentiation factor genes. Introduction of the vector into nonhuman primate iPSCs, such as Macaca fascicularis iPSCs, may initiate transcriptional programs associated with astrocyte-lineage commitment and maturation. Expression of SOX9 and NFIB may facilitate the formation of astrocytic morphology and the development of molecular features associated with astrocytes, including process elaboration and lineage-specific marker expression. The method may further include culturing the transfected cells under conditions that support astrocytic differentiation and expansion, resulting in populations of astrocytes or induced astrocytes suitable for research, disease modeling, and / or therapeutic applications.

The invention provides a construction method of a retinal vein occlusion animal model, and belongs to the technical field of medicines. The method comprises the following steps of: performing eyeball surface anesthesia on an anesthetized non-human primate animal, puncturing an eyeball after disinfection to establish two sclera tunnels, respectively inserting an illumination optical fiber and an intraocular laser optical fiber into the two sclera tunnels, then injecting a photosensitizer through limb vein, immediately performing laser photocoagulation on the retinal vein under illumination of the illumination optical fiber after injection, and performing laser photocoagulation on the retinal vein under the illumination of the illumination optical fiber. And obtaining the retinal vein occlusion animal model. According to the method, the retinal vein occlusion model can be successfully constructed, secondary macular edema and vascular recanalization are carried out for a long time and can be maintained for 4-5 weeks, the manifestation sign of retinal hemorrhage after retinal vein occlusion is close to that of human beings, and the method has clinical drug responsiveness, shows the clinical research value and has good application prospects. And a good research model is provided for research in the aspects of etiology, pathology and therapeutics.