57 results about "Nonhuman primate" patented technology

The utility model discloses a non-contact drinking water system for non-human primates, which comprises a cage, a main line water supply unit and a branch line water supply unit, the main line water supply unit comprises a constant-pressure water source and a drinking water main pipeline, and the drinking water main pipeline is communicated with the interiors of the constant-pressure water source and the branch line water supply unit; one end of the branch water supply unit is communicated with the drinking water main pipeline, and the other end of the branch water supply unit is communicated with the interior of the cage; a water inlet hole is formed in one side of the cage, and the water inlet hole is communicated with the interior of the branch water supply unit. The non-human primate breeding device can well adapt to the breeding scale, funds and breeding technology of the non-human primates, is simple in structure, low in cost, energy-saving and environment-friendly, and can avoid breeding water supply pollution so as to be beneficial to the physical health of the non-human primates.

The invention belongs to the field of biological medicine research and development, discloses a method and a system for collecting and analyzing auditory electrophysiological data of experimental animals, and is particularly suitable for non-human primates. The method comprises the following steps: delivering a drug to a middle ear cavity through an external auditory canal, and synchronously recording auditory electrophysiological data before and after drug administration; aBR and DPOAE multi-mode detection is adopted, and the frequency range of 4 kHz to 32 kHz is covered; setting anesthesia depth and three-electrode positioning to reduce interference; optimizing the signal-to-noise ratio through noise suppression and dynamic signal superposition; an auditory threshold database is constructed, and a machine learning model is combined to judge an injured part. The system integrates a multi-modal stimulation module, a minimally invasive drug delivery device and a data analysis platform, and supports cross-species data compatibility. The accuracy of otology medicine research is remarkably improved, the surgical operation risk is avoided, and the method is suitable for gene therapy, ototoxicity screening and other scenes.

The invention relates to circRNA (Ribonucleic Acid) for coding rabies virus as well as a vaccine and application thereof, and belongs to the technical field of biological medicines. The invention relates to circRNA (Ribonucleic Acid) for coding rabies virus. The nucleotide sequence of the circRNA is shown as SEQ ID NO: 1. The circRNA comprises an SRV9 strain rabies virus G protein sequence after coding optimization, the circRNA is delivered through lipid nanoparticles to form the circRNA-LNP vaccine, powerful and lasting body fluid and cellular immune response can be induced in mice and non-human primates, and the complete protection effect can be achieved through a single dose. Compared with a traditional inactivated vaccine, the vaccine has the advantages of being rapid in production, high in heat stability, capable of inducing Th1 type immune bias and the like, is suitable for preventing rabies virus infection and has wide application prospects and popularization value.

Provided herein are TfR binding VHH polypeptides which bind to human and non-human primate TfR, conjugates thereof, and pharmaceutical compositions comprising these. Provided are also methods of use comprising administering TfR binding VHH polypeptides, conjugates thereof or pharmaceutical compositions thereof to deliver agents, including therapeutic agents, to treat a disease or disorder.

The invention discloses a spleen-targeted nano platform construction method suitable for tumor vaccination, and particularly relates to the field of vaccines.The spleen-targeted nano platform construction method comprises the steps that S1, CL15H6-DOPS LNPs, spherical polymer vesicles and erythrocyte membrane coated nano-particles are selected as spleen-targeted nano-carriers; s2, integrating an immunologic adjuvant; mn < 2 + > doped LNPs, ultrasonic response lipidosome and a CD3 antibody are selected to be coupled to serve as an integration material; s3, delivering in vivo; animal models are selected for in-vivo delivery and curative effect verification, and B16F10 melanoma mice, MC38 colon cancer mice and non-human primates are selected as the animal models respectively. By optimizing the chain length and the surface topological structure (such as spherical polymer vesicles) of the liposome, the spleen enrichment rate is remarkably increased, and the red marrow myeloid cell uptake efficiency is enhanced. By combining with common delivery of a manganese adjuvant, an STING channel is activated, secretion of type I interferon is promoted, and the proportion of antigen-specific CD8 + T cells and the tumor inhibition rate are increased.

The invention relates to a touch fluid neuron specific tracing imaging agent, a preparation method and application, and belongs to the technical field of nuclear medicine imaging, the tracing imaging agent is composed of a nerve tracer agent horseradish peroxidase (HRP), zirconium-89 isotope chelated by deferoxamine and a pharmaceutically acceptable carrier, the effective tracking window of the tracer agent is prolonged to 120 hours, and the effective tracking window of the tracer agent is prolonged to 120 hours. The multi-stage synaptic transfer period of the fluid touching neuron can be completely covered; the spatial resolution is improved by 5-8 times compared with that of traditional FDG-PET, and a fluid touching neuron cluster can be accurately presented; the in-vitro serum stability is excellent, the radiochemical purity is still larger than or equal to 80.5% within 120 hours, PET / MRI bimodal imaging can be adapted, drug administration is conducted through lateral ventricle minimally invasive injection, the probe is suitable for various animal models such as mice, rats and non-human primates, in-vivo dynamic tracing of sympathetic fluid neuron trans-synapses can be achieved on the premise that no tissue damage exists, and the probe has a good application prospect. And a key technical tool is provided for neural circuit analysis, neuroscience basic research and preclinical research of neurodegenerative diseases.

The invention relates to a barcoding method to assess transduction efficiency of AAV vectors, in particular in vivo in rodent and non-human primate.

The invention discloses a sperm genome methylation detection method for safety evaluation of biological breeding crops by using primates and application, and relates to the technical field of safety evaluation of crops, the sperm genome methylation detection method comprises the following steps: dividing non-human primates into three groups, collecting sperms after long-term feeding, and extracting DNA (Deoxyribose Nucleic Acid); carrying out whole genome sequencing and quality control after bisulfite treatment; the epigenetic safety of crops is comprehensively evaluated by analyzing the methylation level of a whole genome and a functional region and functional enrichment of a differential methylation region and related genes thereof; according to the sperm genome methylation detection method for evaluating the safety of the biologically bred crops by utilizing the primates and the application, by utilizing a high-resolution WGBS technology, subtle epigenetic changes which are difficult to find by traditional toxicology can be detected, and the sperm genome methylation detection method has important significance in cross-generation reproduction effect evaluation, and has a wide application prospect. A food safety evaluation system can be perfected, and a more scientific and reliable safety interpretation basis can be established.

The invention provides a puncture device for a pharmaceutical injection test, and relates to the technical field of medical experiments, the puncture device comprises a base mechanism, the base mechanism is fixed on a forearm of a non-human primate experiment body through a first medical adhesive tape and a second medical adhesive tape, and the base mechanism is fixedly provided with an injection tube through a locking mechanism; a middle connecting pipe is installed at one end of the injection pipe, a separation non-return hose is inserted into the end, away from the injection pipe, of the middle connecting pipe, and a needle mechanism is fixedly installed at the end, away from the middle connecting pipe, of the separation non-return hose. Through the cooperation of the base mechanism, the first medical adhesive tape, the second medical adhesive tape and the silica gel pad, comfortable and stable attachment of the device to the forearm of an experimental body is achieved, pulling caused by animal movement is effectively resisted, and through rigid locking of a threaded inclined plate and a fastening nut of the locking mechanism to an injection tube clamping convex ball, the clamping convex ball can be locked more firmly. And the reliability of connection between the infusion tube and the middle connecting tube is further ensured.

The invention relates to the technical field of non-human primate cognitive testing devices, in particular to a non-human primate-based behavioral training and testing device and method. The device comprises a fixing frame, a screen unit, a grabbing rod reward unit, a sensing unit, a transition cage and a controller. Wherein the screen unit comprises a screen and a screen clamping groove, and the screen is connected with one side of the fixing frame through the screen clamping groove; the grabbing rod reward unit comprises a grabbing rod frame, a grabbing rod, a liquid conveying hose, a suction nozzle hole, a peristaltic pump and a reward liquid carrier, one side of the grabbing rod frame is fixedly connected with the other side of the fixing frame, and the grabbing rod is connected with the grabbing rod frame; the sensing unit comprises an infrared sensor unit and a camera shooting unit; the transition cage comprises a movable plate and a cage body, and the movable plate is movably connected with the cage body; the controller is in communication connection with the screen, the sensing unit and the peristaltic pump. The test efficiency can be improved, the test error is reduced, the stress response is reduced, and the experiment reliability and flexibility are improved.

This application relates to a modular non-human primate rearing cage, belonging to the technical field of animal husbandry. It includes a cage frame, a resource supply system, a hygiene and cleaning system, an activity and enrichment system, and a connection and management system. The cage frame includes a cage base, a cage top, and cage side frames. The resource supply system includes a food supply device and a water supply device detachably installed on the cage frame. The connection and management system includes a connecting channel on the cage side frames and a connecting cage door movably installed on the cage side frames for changing the opening and closing state of the connecting channel. The activity and enrichment system includes a lookout component installed on the cage side frames, a climbing component located between the lookout component and the cage base, and multiple monitoring units installed on the cage top frame. This application can improve the husbandry and management efficiency of non-human primates.

A method and system are disclosed for differentiating non-human primate induced pluripotent stem cells (NHP iPSCs) into hepatocyte-like cells through a defined, multi-stage protocol. The method includes sequentially exposing NHP iPSCs to six distinct cell differentiation media over an approximate eighteen-day period. Each medium comprises defined combinations of growth factors, small molecules, and inhibitors that direct progressive lineage specification through anterior primitive streak, definitive endoderm, posterior foregut, liver bud progenitors, hepatoblasts, and hepatocyte-like cells. Concentration ranges and exposure durations are provided for each stage, ensuring reproducibility and reducing heterogeneity across cell populations. In some embodiments, treatment with a transforming growth factor beta (TGF-β ) receptor I inhibitor during early hepatoblast specification enhances lineage fidelity. The resulting hepatocyte-like cells are validated by immunostaining and western blotting for hepatic markers. Applications include preclinical drug metabolism, hepatotoxicity assessment, and in vitro disease modeling using non-human primate–derived systems.

The utility model discloses a device for testing the lower limb muscle strength of a non-human primate animal, and the device comprises a device body which comprises an animal fixing box, the back of the animal fixing box is provided with an animal access door, and the top of the animal fixing box is provided with an animal head fixing device; through holes allowing the arms of the animals to extend out are symmetrically formed in the two sides of the animal fixing box, a muscle strength testing device and a fixing ring used for being fixed to the ankles of the animals are arranged in the animal fixing box, and the muscle strength testing device is connected with the fixing ring and used for detecting lower limb muscle strength data of the non-human primates. The lower limb muscle force data of the non-human primate animal can be accurately recorded, so that the recovery degree of the lower limb muscle force of the non-human primate animal can be scientifically and objectively observed, and objectivity, scientificity and accuracy of animal medical experiment data are facilitated.

The invention provides a targeting reagent for preparing fragile X syndrome animals and application of the targeting reagent. The invention discloses a target which is matched with non-human primates and is suitable for gene modification, namely a third exon and / or a fourth exon of an FMR1 gene, a target reagent is prepared by targeting the target, and a fragile X syndrome animal model with typical disease symptoms can be obtained by implementing down-regulation.

This invention discloses a neural microprobe for bimodal functional localization and precise electrical stimulation of deep brain nuclei in non-human primates. It comprises a basal layer, a conductive metal layer, and an insulating layer. The conductive layer includes a set of detection sites and stimulation sites. This invention employs a dry etching process on the back of the silicon basal layer using two insulating layers with opposite stresses, balancing the stress differences between the different materials. This overcomes the technological challenges of extending silicon microprobes and solves the problems of probe bending and low mechanical stress caused by the large aspect ratio of silicon probes in non-human primates. The detection sites acquire bimodal signals using modified nanomaterials and polymer anti-interference ion-exchange membranes as localization markers for functional localization. The detection sites are arranged between the positive and negative electrodes of the electrical stimulation, controlling the stimulation range to be near the detection sites, thus achieving precise electrical stimulation. This invention provides a new research method and means for deep brain stimulation therapy of diseases such as Parkinson's disease.

The invention relates to the field of medicines, in particular to a construction method of an Alzheimer's disease non-human primate model. A small dose of thiamine antagonist pyrithiamine is given to the chronic abdominal cavity to induce chronic thiamine metabolic disorder, so that the non-human primate marmoset Alzheimer disease model capable of reflecting the Alzheimer disease course is established, and the method has the advantages of simplicity in operation, small animal injury and the like; therefore, the method is beneficial to further basic clinical transformation research and the like, and has more important scientific significance and economic value.

The feeding barrel for the non-human primates comprises a barrel body and a barrel cover detachably installed on the barrel body, the barrel body is in a barrel shape with the top open, and the side wall of the barrel body is hollowed out so that the feeding position can be observed conveniently; the barrel cover is detachably hung on the top wall of the rearing cage, and the barrel body and the barrel cover are made of food-grade hard materials. According to the feeding barrel for the non-human primates, food is placed in the feeding barrel, so that the food is effectively prevented from being contaminated by dirt in a rearing cage; the hand and the arm of a non-human primate extend into the feeding barrel from the feeding hole for feeding, and meanwhile, the position of food is observed through the hollow structure, so that foraging is facilitated, the fun of exploring and finding in the foraging process of the non-human primate is increased, and animal welfare is increased; the barrel body and the barrel cover are preferably made of food-grade hard materials, can be effectively prevented from being damaged by non-human animals, are convenient to clean and meet the strict requirements of industries such as medicine research and development on sanitary conditions.

A method for differentiating non-human primate iPSCs into neurons may include providing a modified vector containing a piggyBac vector backbone, the modified vector expressing differentiation factor genes; treating the non-human primate iPSCs in a culture medium with the modified vector; replacing the culture medium with a second culture medium comprising BDNF, NT-3, and laminin; adding a culture medium supplemented with L-glutamine or an L-glutamine substitute, BDNF, and NT3; and generating neuronal lineage cells.

A method of differentiating nonhuman primate induced pluripotent stem cells (iPSCs) to oligodendrocytes is provided. The method may include providing a modified vector comprising a piggyBac vector backbone that expresses one or more differentiation factor genes, including SOX10, OLIG2, and NKX6-2. The piggyBac vector backbone may comprise one or more terminal inverted repeats and one or more transposase recognition sites configured to interact with a piggyBac transposase to mediate genomic integration at TTAA or noncanonical target sequences. The vector may further include a selectable marker and a promoter for regulated expression. The modified vector may be introduced into nonhuman primate iPSCs, for example from Macaca fascicularis, by electroporation and used in a feeder-free differentiation workflow. The resulting differentiated cells may be identified by expression of oligodendrocyte-associated markers including O4, O1, PDGFRA, MOG, GALC, CNPase, and myelin basic protein (MBP).

Disclosed are an antibody targeting the monkeypox virus, an antigen-binding fragment thereof, and a use thereof. The antibody or the antigen-binding fragment thereof comprises a heavy chain variable region and a light chain variable region. The antibody or the antigen-binding fragment thereof of the present invention targets A35R of humans and non-human primates.

The application belongs to the field of animal research, and discloses a non-human primate cooperation experiment method and device, which comprises the following steps: step one, using open source single-chip microcomputer code editing software to write required cooperation logic code and burning the code into a circuit board; step two, starting the animal cooperation experiment device; step three, an animal operating a pressing part; step four, judging the triggering condition of the pressing part, if the condition meets the cooperation logic, the cooperation experiment device outputs food, otherwise, the cooperation experiment device outputs a punishing content; and step five, resetting the animal cooperation experiment device; when multiple non-human primates complete cooperation, the application uses food as a reward to encourage cooperation among animals, so that the cooperation mode among non-human primates can be studied, the food stored in the food bin can be supplemented into the temporary storage cavity after the reward, so that the temporary storage cavity always has food, and the device has a small volume and can be installed outside a monkey cage for use at any time.

The utility model provides a mobile non-human primate weighing device which comprises a bottom frame, a base, a support and an operation platform, the base is detachably arranged on the bottom frame, the support is arranged on the base, the operation platform is arranged on the upper portion of the support, and the base can be placed on a platform scale after being detached; the support comprises a connecting square pipe, at least one set of grabbing rods and a mounting support, the bottom of the connecting square pipe is connected with the base, the grabbing rods are arranged in the middle of the connecting square pipe, and the mounting support is arranged on the top of the connecting square pipe. Due to the design of the grabbing rod, the grabbing response under the conditions that non-human primates like to climb and are tense is met, and the operation process is more stable or rapid adaptation is achieved in the weighing process. The movable animal cage is simple in structure, reasonable in design, easy to fix animals and high in moving performance due to the fact that the open type frame structure is adopted, and meanwhile the anti-collision corners are installed at the four corners so that used facilities can be protected.

The invention discloses a construction method and application of a dog depression model. According to the method, the problems of dog allergy and fever in the modeling process are solved by injecting corticosterone through stepped increasing administration, and the dog depression model is successfully constructed for the first time. A large number of experiments prove that the dog depression model established by the invention successfully simulates the core phenotypes of human depression, such as pleasant sensation deficiency, low emotion, reduced interest and the like, and the major depression phenotype self-disability behavior appears for the first time. The method is simple, the molding period is greatly shortened, the success rate is increased to 80%, drug screening and pharmacology and toxicology synchronous research are supported, and the drug research and development cost is reduced. According to the invention, the problems of low clinical depression correlation and long non-human primate large animal modeling period of the existing mouse model are solved, and a standardized large animal platform with high prediction value is provided for research and development of antidepressant drugs.

The invention provides a non-human primate fine motion and posture tracking automation method, which is implemented by taking a monkey as a representative, and comprises the following steps: defining 10 motion and 3 posture labels of the monkey, defining 15 key points of the monkey body, constructing a monkey motion identification data set and a monkey key point data set, and establishing a monkey motion and posture identification analysis tool box. The monkeys are subjected to action recognition and skeleton point recognition, and the three postures of platform, sitting and curling up of the monkeys, the engraving behavior and the depression behavior are automatically recognized. According to the invention, automatic identification of fine actions and postures of the non-human primates can be effectively realized, and particularly, effective automatic identification of engraving behaviors, depression behaviors and the like of the non-human primates can be realized, so that the blank of such researches in the field is filled; the method is of great significance to clinical research of difficult miscellaneous diseases such as depression and Rett syndrome.

The invention belongs to the technical field of regenerative medicine, and discloses an organoid culture method derived from neural stem cells in subventricular regions of human and non-human primates. By optimizing a culture medium formula and regulating and controlling transcription factors, the organoid is successfully induced to be differentiated into the neuron precursor for expressing the CRABP1. The cell expresses TAC3, the limitation of the prior art in simulation of special neuropedigree and complex brain structure of primates is overcome, and a breakthrough in-vitro model is provided for research on neurodevelopment and nervous system diseases and drug development. The established system not only can provide a new tool for researching the development law and disease mechanism of the primate nervous system, but also can provide a reliable experimental platform and a transplantable cell source for developing targeted drugs, constructing advanced disease models and developing cell replacement therapy.

The application discloses a non-human primate cage door locking device, which comprises a cage door, a T-shaped bolt lock and a locking structure. The cage door is provided with a plurality of cylindrical sleeves. The T-shaped bolt lock comprises a lock core part and a handle part. The lock core part is a horizontal circular rod in the axial direction, and the handle part is a vertical circular rod in the axial direction. The top end of the handle part is welded at the midpoint of the lock core part. The two ends of the lock core part are respectively inserted into the cylindrical sleeves. The T-shaped bolt lock can move left and right relative to the cage door. A through hole is formed in the T-shaped bolt lock. One end of the locking structure penetrates through the through hole and is detachably mounted on the T-shaped bolt lock. The non-human primate cage door locking device has the advantages of simple structure, easy operation, low cost, easy manufacturing, difficult learning of the opening and closing mode by monkeys, prevention of the monkeys from opening and closing the cage door by themselves, great improvement of the working efficiency of non-human primate cage production personnel and solution to the difficult problems in the intensive feeding of non-human primates.

The invention relates to the field of medicines, in particular to a construction method of an Alzheimer's disease non-human primate model. A small dose of thiamine antagonist pyrithiamine is given to the chronic abdominal cavity to induce chronic thiamine metabolic disorder, so that the non-human primate marmoset Alzheimer disease model capable of reflecting the Alzheimer disease course is established, and the method has the advantages of simplicity in operation, small animal injury and the like; therefore, the method is beneficial to further basic clinical transformation research and the like, and has more important scientific significance and economic value.

This invention discloses a non-human primate visual-spatial working memory testing cage. This invention uses a non-human primate visual-spatial working memory testing cage to measure the learning, discrimination, and reversal abilities, or cognitive and memory abilities, of non-human primates, providing a more comprehensive evaluation of changes in animal brain function and behavior. It can provide a model evaluation tool for central nervous system diseases such as Alzheimer's disease and stroke, and offer new methods for disease research and new drug development.

Provided herein are TfR binding VHH polypeptides which bind to human and non-human primate TfR, conjugates thereof, and pharmaceutical compositions comprising these. Provided are also methods of use comprising administering TfR binding VHH polypeptides, conjugates thereof or pharmaceutical compositions thereof to deliver agents, including therapeutic agents, to treat a disease or disorder.

The utility model relates to the technical field of animal experiments, in particular to a small non-human primate fixing plate which comprises a plate body and a plurality of bandage structures, and the plate body is provided with a plurality of through hole structures for the bandage structures to penetrate through. The perforated structures are at least arranged in the first area, the second area and the third area of the plate body, and at least two perforated structures which are arranged left and right and have a preset interval are arranged in each area. The fixing device is free of metal materials, the bandage structure and the plate body are simple and convenient to connect, the length between the two bandages is flexible to adjust, the fixing device has better adaptive capacity for different animals, rapid adjustment and fixing can be achieved during fixing, meanwhile, the trunk or the four limbs cannot be pressed, and the fixing device is convenient to use. And the use of the experimental animal in the medical, examination or transportation process can be met, and meanwhile, the advantages of safety and comfort are achieved.