Papillomavirus detection and parting method as well as liquid phase chip thereof

Abstract

The invention discloses papillomavirus detection and a typing liquid chip, and a process which is used to the detection and typing papillomavirus. The liquid chip mainly comprises microsphere which is respectively enveloped with specific anti-tag label sequence and 7-10 T spacer arm sequence which is arranged between anti-tag label sequence and microsphere, wherein anti-tag label sequence is chosen from two or more than two sequences in SEQ ID NO. 25- SEQ ID NO.47, specific ASPE primer is respectively designed aiming at each typing HPV, ASPE primer is chosen from two or more than two sequence from SEQ ID NO.1-SEQ ID NO.23, and primer of target sequence which has mutant sites is amplified. The liquid chip improves the current liquid chip technology, which makes ribonucleotide probe microspheres which is prepared be suitable to different detection projects, largely increases fluorescent signal value which is detected, thereby further increasing sensitivity of the detection, strengthening signal-to-noise ratio, and making detection results more accurate and reliable.

Description

technical field [0001] The invention relates to medical in vitro diagnostic technology, in particular to a method for detecting and typing human papillomavirus and a liquid phase chip thereof. Background technique [0002] Human papilloma virus (HPV) is a small-molecule circular double-stranded DNA virus that spreads widely among people through sexual contact, direct or indirect contact with contaminated objects, and specifically infects human skin and mucous membranes. Squamous epithelial cells can cause a variety of benign or malignant lesions, which seriously threaten human health. According to the sequence variation of HPV gene, it can be divided into many types. There are more than 100 types of HPV identified so far, about 40 of which can infect human reproductive organs, and about 20 are related to tumors. According to the risk of different types of HPV and tumor occurrence, it is divided into high-risk type and low-risk type. Low-risk HPV types such as HPV6, 11, 40,...

AI Technical Summary

Problems solved by technology

Due to the need to coat specific oligonucleotide probes on the microspheres, the coating step is increased, and the coated microspheres can only be used for papillomavirus HPV typing

In addition, the existing HPV detection liquid chip technology adopts the method of labeling biotin at the 5' end during PCR amplification, so the fluorescence signal is relatively low, which limits the sensitivity of detection

At the same time, after PCR amplification, the redundant free nucleotides (such as biotin-labeled primers and dNTPs) in the system were not processed, which made the subsequent hybridization reaction more likely to be non-specific, thus making the signal noise of the detection results Comparison

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