Application of melatonin to influence on rhizosphere soil microorganism diversity of medicinal plants

A technology of medicinal plants and rhizosphere soil, applied in the direction of chemicals for biological control, plant growth regulators, plant growth regulators, etc., can solve the problems of slow growth, low germination rate, serious diseases, etc. The content, preparation and use are simple, and the effect of promoting metabolism

Active Publication Date: 2021-06-25
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To sum up, low germination rate, slow growth, long cycle, serious disease, and poor stress resistance are the main reasons restricting the development of the artificial planting industry of Chonglou

Method used

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  • Application of melatonin to influence on rhizosphere soil microorganism diversity of medicinal plants
  • Application of melatonin to influence on rhizosphere soil microorganism diversity of medicinal plants
  • Application of melatonin to influence on rhizosphere soil microorganism diversity of medicinal plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, a kind of application melatonin impels the seedling breeding technology of the chrysanthemum seedling of breaking dormancy and accelerating germination

[0039] 1. Seed treatment

[0040] In mid-September, 2018, fresh Huazhonglou seeds were collected, and the rotten seeds were removed for later use (experimental group); the seeds of the control group were rotten and red seed coats were removed for later use. Preparation of mixed substrate: 0-10mm seedling peat soil, granular vermiculite, and rice husk charcoal in a volume ratio of 3:1:1. Mix the above-mentioned two kinds of seeds with seed coat and seed coat removed with the clean mixed medium at a volume ratio of 1:1, put them into a breathable plastic frame with a depth of 15cm, pour water thoroughly, and store at room temperature . Five box replicates were set for each treatment.

[0041] The seeds stored in the experimental group were stored for about 10 days, at a depth of 5cm, and the temperature ...

Embodiment 2

[0056] Example 2. Determination of Physiological and Biochemical Indexes of Pachyphyllis Pachyphylli Leaf after Treatment with Melatonin

[0057] The malondialdehyde content detection kit used in the detection of this embodiment, the chlorophyll content detection kit, the hydrogen peroxide content detection kit, the superoxide dismutase activity detection kit, the peroxidase activity detection kit and the peroxide Hydrogenase activity detection kits were purchased from Suzhou Keming Biotechnology Co., Ltd.

[0058] As a signal molecule, hydrogen peroxide can promote the growth of adventitious roots and the growth and expansion of underground parts of rhizome crops, and enhance the resistance to biological stress; there is a certain positive correlation between chlorophyll content and biomass; malondialdehyde is an important indicator of cell membrane damage. Index; superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) are the main reactive oxygen species scavenging syst...

Embodiment 3

[0068] Example 3. Determination of endogenous hormones in leaves of Chrysanthemum chinensis after melatonin treatment

[0069] Test grouping, test method etc. are completely identical with embodiment two, leaf endogenous salicylic acid, jasmonic acid, abscisic acid, gibberellin 3, 4, 7, trans-zeatin, trans-zeatin nucleoside, zeatin, 6- Furfuryl aminopurine, N6-prenyl adenine, lipoadenosine, indole acetic acid, 4-chloro-indole acetic acid, indole propionic acid and indole butyric acid, of which, salicylic acid, jasmonic acid, absciss Acid is related to plant stress, gibberellin, cytokinin and auxin are related to plant growth, salicylic acid is the main endogenous signal molecule of plant under biological stress, abscisic acid is the main endogenous signal molecule of drought stress, jasmonic acid It is an endogenous signal molecule for mechanical damage and insect pests. Because it has a positive synergistic effect with ethylene, it also has a certain effect on abiotic stress....

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Abstract

The invention relates to the technical field of biology, in particular to application of melatonin serving as an effective component of a foliar spraying agent to influence on rhizosphere soil microorganism diversity of medicinal plants. The content of the melatonin in the foliar spraying agent is 100-300 mg / L, and an inducer further contains 0.05 g / L of hydroxypropyl methyl cellulose. The melatonin can reduce the content of endogenous salicylic acid and hydrogen peroxide of plants, increase the content of jasmonic acid, promote proliferation and endosymbiosis of rhizosphere probiotic fungi and change the rhizosphere soil microorganism diversity of the paris polyphylla. In conclusion, when the melatonin is sprayed on the leaf surfaces, the composition of rhizosphere microorganisms can be adjusted by changing the endogenous hormone level, so that the effects of resisting diseases and promoting growth of the paris polyphylla are achieved. The foliar spraying agent taking the melatonin as the effective component is used for cultivating paris polyphylla var chinensis and has the effects of resisting diseases and promoting growth, the preparation and use methods are simple, the cost is low, and popularization and application can be realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of melatonin to affect the diversity of microorganisms in the rhizosphere soil of medicinal plants. Background technique [0002] The traditional Chinese medicine Zhonglou is the dried rhizome of Paris polyphylla Smith var. Steroidal saponins are the main active substances of Chonglou, which have pharmacological effects such as anti-tumor, anti-inflammation, hemostasis, anti-oxidation, promotion of uterine contraction and protection of vascular endothelial cells. Chonglou has strict requirements on the growth environment, likes warm and humid, and is afraid of direct sunlight; likes loose, fertile, humus-rich soil, and avoids hardened, waterlogged soil. There are about 20 kinds of heavy buildings distributed in our country. Among them, the species that are widely distributed include: Polyphylla multi-leaf, Parisia yunnanensis, and Parisia macrophylla. In Lijiang are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01G22/25A01G24/28A01G24/15A01G24/10A01N43/38A01N43/12A01N37/10A01N43/22A01P21/00
CPCA01G7/06A01G22/25A01G24/28A01G24/15A01G24/10A01N43/38A01N43/12A01N37/10A01N43/22Y02P60/21
Inventor 杨涛方彦昊杨晖毛婷牛永艳赵疆李鑫
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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