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Efficient turfgrass endogenous hormone separation and determination method

A technology of endogenous hormones and turfgrass, which is applied in the direction of material separation, measuring devices, and analysis materials, and can solve the problem that it is difficult to meet the needs of rapid extraction and detection of various turfgrass endogenous hormones, complex detection operations of plant endogenous hormones, and recycling. Low efficiency and other problems, to achieve the effect of less chemical reagent consumption, high recovery rate and cost saving

Inactive Publication Date: 2014-05-28
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology

[0007] The purpose of the present invention is mainly to provide a high-efficiency separation and determination of multi- Method for growing lawn grass endogenous hormone

Method used

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  • Efficient turfgrass endogenous hormone separation and determination method
  • Efficient turfgrass endogenous hormone separation and determination method
  • Efficient turfgrass endogenous hormone separation and determination method

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Embodiment Construction

[0023] Below in conjunction with embodiment describe in detail:

[0024] 1. Test material: Bermudagrass material treated with salt stress (treatment concentration: 200mmol / L NaCl) for 35 days (C43 bermudagrass from the Germplasm Resource Garden of Wuhan Botanical Garden, Chinese Academy of Sciences).

[0025] 2. Detection steps

[0026] 1) Extraction of endogenous hormones

[0027] A. Extraction: Take 0.5g of fresh sample, grind it with liquid nitrogen, then add 5ml of extraction solution, and extract overnight at -20°C to obtain the extraction solution;

[0028] The extract is methanol: formic acid: water = 15:1:4, according to v / v, plus 40mg / ml 2,6-di-tert-butyl-4-methylphenol as an antioxidant;

[0029] B. Centrifugation: Centrifuge the extract at 15,000 rpm and 4°C for 10 minutes, collect the supernatant and store it in a new tube at -20°C, extract the residue with 5ml of extract at -20°C for 1 hour, and centrifuge. Collect the supernatant and mix it with the previous s...

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Abstract

The invention discloses an efficient turfgrass endogenous hormone separation and determination method, which relates to the field of the determination of plant endogenous hormones. The method comprises the steps as follows: (1) an extraction liquid is added to perform leaching, and the endogenous hormone is extracted via centrifugation, filtration, decoloration and vacuum concentration; (2) the endogenous hormone is dissolved to obtain a solution, the solution passes through a 96-hole solid-phase extraction small column to be gradually eluted, an eluant is subjected to vacuum concentration so as to obtain a solid-phase extraction eluant; (3) the solid-phase extraction eluant is dissolved with a flux phase, and the obtained flux-phase solution is filtered and loaded on an efficient liquid-phase chromatogram-mass spectra to perform sample analysis. The method is high in detection sensitivity and good in selectivity, needless in derivation, and can directly detect the endogenous hormone of a plant in a crude extraction liquid of a plant sample; a separation and extraction way, chromatogram separation conditions and a mass spectra detection way of the method are suitable for the determination of the turfgrass endogenous hormone, and the recovery rate is high and can reach 92-96 percent; the method can quickly and accurately analyze the contents of the endogenous hormone of four kinds of turfgrass endogenous hormones simultaneously.

Description

technical field [0001] The invention relates to the field of determination of plant endogenous hormones, in particular to a method for efficiently separating and measuring turfgrass endogenous hormones. Background technique [0002] With the development of my country's economy and the strengthening of ecological environment awareness, as well as the acceleration of urbanization and the improvement of urban greening standards, the development of the turf industry has ushered in a good opportunity, and the demand for turfgrass continues to increase. As an important part of the urban ecosystem, the lawn can purify the air, sterilize, reduce noise, prevent soil erosion and improve the microclimate in the prevention and control of pollution and the improvement of the ecological environment, and has a strong ornamental and recreational value. . In today's frequent smog, how to improve our living environment has a long way to go, and turfgrass plays an important role in improving ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 傅金民胡龙兴娄燕宏
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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