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Medium and method for tissue culture and rapid propagation of kiwifruits

A technology of proliferation medium and medium, applied in the field of plant tissue culture, can solve the problems of low survival rate of seedlings, difficulty in rooting, easy to grow callus, etc., so as to improve production efficiency, shorten the period of rooting and seedling training, and improve transplanting. The effect of planting survival rate

Active Publication Date: 2018-12-18
陕西青美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for kiwifruit to take root in traditional tissue culture, and it is easy to produce abnormal roots, aerial roots, and roots to grow calluses, resulting in extremely low survival rate of seedlings.

Method used

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  • Medium and method for tissue culture and rapid propagation of kiwifruits
  • Medium and method for tissue culture and rapid propagation of kiwifruits
  • Medium and method for tissue culture and rapid propagation of kiwifruits

Examples

Experimental program
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Effect test

Embodiment 1

[0052] A rapid propagation medium for kiwifruit, including a medium for inducing leaf differentiation callus, a medium for inducing shoot tip or axillary bud germination growth, a medium for inducing callus differentiation to cluster buds, a medium for cluster bud proliferation, and sugar-free tissue culture to induce adventitious roots Culture medium, among them,

[0053] Callus medium for inducing leaf differentiation: MS medium 1 liter, 6-benzylaminoadenine 0.8-1.2mg / L, 2,4-dichlorophenoxyacetic acid 0.3-0.6mg / L, sucrose 25-30g / L , agar 5.8-6g / L; when preparing, first measure MS medium, add 6-benzylaminoadenine, 2,4-dichlorophenoxyacetic acid, add sucrose, and adjust the pH to 5.8 after the sucrose dissolves Between -5.9, add agar, heat to melt the agar, and sterilize under high temperature and high pressure at 121°C for 21min. When in use, cultivate under light (12h, 2000Lx), and the cultivation temperature is 21-25°C.

Embodiment 1-1

[0055] Callus induction medium for leaf differentiation: first measure 1 liter of MS medium, add 0.8 mg of 6-benzylaminoadenine, 0.3 mg of 2,4-dichlorophenoxyacetic acid, and 25 g of sucrose. After the sucrose is dissolved, adjust When the pH reaches 5.8, add 5.8 g of agar, heat to melt the agar, and sterilize under high temperature and high pressure at 121° C. for 21 minutes.

Embodiment 1-2

[0057] Callus induction medium for leaf differentiation: first measure 1 liter of MS medium, add 1.2 mg of 6-benzylaminoadenine, 0.6 mg of 2,4-dichlorophenoxyacetic acid, and 30 g of sucrose. After the sucrose dissolves, adjust When the pH reaches 5.9, add 6 g of agar, heat to melt the agar, and sterilize under high temperature and high pressure at 121° C. for 21 minutes.

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Abstract

The invention discloses a medium and a method for tissue culture and rapid propagation of kiwifruits. The medium for tissue culture and rapid propagation of kiwi fruits comprises a leaf induced differentiation callus culture medium, stem tip or axillary bud inducing germination growth medium, a callus induced differentiation cluster bud medium, a cluster bud proliferation medium and a sugar-free tissue culture induction adventitious root medium. An endogenous hormone rooting technology is adopted, a sugar-free nutrient solution is added into a specific culture container, and the micro-environment is manually controlled, so the rooting and seedling-hardening cycle is shortened, and the transplanting survival rate is improved. Obtained seedlings grow vigorously, have a large root system, andcan be directly transplanted into a nutrition pot or field without being hardened. The production efficiency of a sugar-free rooting technology is 18 times or more that of conventional sugar rootingtechnologies, so the production efficiency of the large-scale production of virus-free kiwifruit seedling is greatly improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a kiwi fruit tissue culture rapid propagation medium and method. Background technique [0002] Actinidia belongs to Actinidiaceae, and Actinidia belongs to deciduous vines. It is an important economic forest and fruit tree. Kiwi fruit is rich in nutrients such as Vc, minerals and anthocyanins, and has high nutritional and medicinal value. In recent years, it has been widely used in edible forms such as fresh fruits, beverages, and health care products, and the market demand is relatively large. The promotion of planting can meet the needs of more consumers and increase the economic benefits of fruit farmers. However, the long growth cycle of sowing and reproduction and the long age of flowering are not conducive to the rapid formation of the market value of kiwifruit. [0003] Tissue culture is a method of rapid clonal reproduction, which has the characteristics o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 段珍珍
Owner 陕西青美生物科技有限公司
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