Culture media for promoting induction and rooting of cluster buds of dendrobium candidum and culture method by using rare earth elements
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A technology of rooting medium and induction medium, applied in the field of induction and rooting culture, can solve the problems of low transplant survival rate and low seedling emergence rate, and achieve the effects of increasing the number of seedlings, improving the seedling emergence rate and promoting rooting.
Active Publication Date: 2015-11-04
NANJING NORMAL UNIVERSITY
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Aiming at the problems of low seedling emergence rate and low transplant survival rate in the prior art, the present invention provides a practical solution
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Embodiment 1～8
[0028] (1) the culture medium that the required induction cluster bud of preparation example 1~8
[0029] The culture medium for inducing clustered buds needed for examples 1 to 8 is prepared by adding agar, white granulated sugar, activated carbon, Huabao No. 2, banana juice, potato juice and samarium nitrate to 1 / 2MS medium. Huabao No. 2 , banana juice, potato juice, and samarium nitrate are all shown in Table 1 for the concentrations in the culture medium for inducing clustered buds required in Examples 1 to 8. The medium numbers 1 to 8 for inducing clustered buds in Table 1 respectively correspond to the mediums for inducing clustered shoots in Examples 1 to 8. After preparing the culture medium for inducing clustered buds of Examples 1-8, they were respectively packed in culture bottles No. 1-8, sterilized by high pressure and cooled for later use.
[0030] Table 1 Concentration of each component in the medium for inducing clustered buds
[0031]
[0032] (2) the req...
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Abstract
The invention provides culture media for promoting induction and rooting of cluster buds of dendrobium candidum and a culture method by using rare earth elements. The induction culture medium is prepared by adding a certain amount of Huabao #2, samarium nitrate, banana juice, potato juice, active carbon, agar and white granulated sugar into a 1 / 2 MS culture medium; the rooting culture medium is prepared by adding a certain amount of Huabao #2, europium nitrate, banana juice, potato juice, active carbon, agar and white granulated sugar into the 1 / 2 MS culture medium; According to the culture media and culture method provided by the invention, a proper amount of rare earth elements is added into the culture media, and the proper amount of rare earth elements can increase the content of endogenous hormones of plants, so that the activity of various enzymes is enhanced, the respiratory action of the plants is improved, and H2O2 in tissues is effectively eliminated, and therefore, biomass increase of tissue culture seedlings is promoted, and more tissue culture seedlings with thick roots are obtained. The method has the advantages of being simple and feasible, short in rooting time, developed in root system and economical and practical, and more plants can be obtained, so that the tissue culture seedling survival rate of transplanting can be greatly improved, and the requirement on intensive production is satisfied.
Description
technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a cultivation method for the induction and rooting of clustered buds of the medicinal plant Dendrobium officinale, which can effectively increase the emergence rate and improve the survival rate of transplanting. Background technique [0002] Dendrobium officinale, also known as black knot grass, belongs to the epiphytic orchid of Orchidaceae. It is a rare medicinal plant, which has the functions of nourishing yin and promoting body fluid, moistening throat and protecting throat, warming stomach and improving eyesight. It is a precious and endangered species under the national second-level protection One of the medicinal materials, it is regarded as "the top grade of medicine" in the medical classics of all dynasties. The medicinal part of Dendrobium candidum is fresh or dried stems. Modern medical research shows that Dendrobium candidum can significantly improve th...
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