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Method for measuring content of endogenous hormones of burgeons of tea tree

A technology of endogenous hormones and determination methods, which is applied in the field of determination of endogenous hormones in tea tree terminal buds based on high performance liquid chromatography, which can solve the problems of less endogenous hormones, interference of hormone determination, preparation of mobile phase, and high requirements for setting chromatographic conditions, etc. question

Active Publication Date: 2015-09-09
湖北省鹤峰县翠泉茶业有限公司
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Problems solved by technology

High-performance liquid chromatography is fast, efficient, high-sensitivity, and highly automated, but it has high requirements for preparing mobile phases and setting chromatographic conditions; due to the presence of polyphenolic substances such as tea polyphenols in tea buds, biochemical components will affect The determination of hormones causes interference, so the pretreatment of its samples is different from that of other varieties
At present, there are few previous studies on the method of using high performance liquid chromatography to determine the endogenous hormones of tea tree terminal buds, and there is no suitable determination method.

Method used

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  • Method for measuring content of endogenous hormones of burgeons of tea tree
  • Method for measuring content of endogenous hormones of burgeons of tea tree
  • Method for measuring content of endogenous hormones of burgeons of tea tree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] The first step: tea tree bud sample collection

[0037] After different nitrogen fertilizer treatments (0.4g / pot, 0.8g / pot, 1.6g / pot, 2.4g / pot) of tea tree shoots, take one bud and two leaves, put them in liquid nitrogen for 5 minutes, and put them in the refrigerator to freeze. Store frozen at -24°C. The role of liquid nitrogen is mainly to fix the endogenous hormones in the shoots.

[0038] The second step: equipment and medicine preparation

[0039] (1) Instruments and equipment

[0040] 10-1000μL DRAGONLAB adjustable pipette, Shanghai Wandao Instrument Technology Co., Ltd.;

[0041] 1000mL sand core filter device, Tianjin Jinteng Experimental Equipment Co., Ltd.;

[0042] QP-01 small vacuum pump, Shenzhen Liangyi Instrument Company;

[0043] DS-5510DTH ultrasonic cleaner, Shanghai Aoxi Scientific Instrument Co., Ltd.;

[0044] LC600 liquid chromatograph, Beijing Labtech Instrument Co., Ltd.

[0045] (2) Pharmaceutical reagents

[0046] IAA standard product, ...

example

[0074] Example: 6 replicate injections per sample. Among the spring shoots of tea tree variety Wuniuzao, GA 3 The content is 0.654μg / gFW (fresh weight), the content of ZT is 0.412μg / gFW, the content of IAA is 0.585μg / gFW, and the content of ABA is 1.35μg / gFW.

[0075] Step 7: Test of reproducibility and precision

[0076] The same batch of tea tree bud sample stock solution was taken, and after adding the mixed standard stock solution, the contents of four kinds of tea tree bud hormones were determined by the peak area according to the external standard method, and the recovery rate of the addition was calculated.

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Abstract

The invention discloses a method for measuring the content of endogenous hormones of burgeons of a tea tree on the basis of high performance liquid chromatography (HPLC). Conditions such as composition, concentration and flow speed of a flowing phase and the adding proportion and the detection wavelength of organic modifying agents are optimized, an optimal chromatographic condition of the content, which is measured by the HPLC, of the endogenous hormones of the burgeons of the tea tree is determined, and repeatability and precision of the method are inspected. The method has characteristics of high speed, high efficiency, high sensitivity and high automation; a tea tree terminal bud sample preparation method and selection and chromatographic conditions of the flowing phase are explored when the hormones of terminal buds of the tea tree are measured by the high performance liquid chromatography, and the method for measuring plant hormones by using the HPLC successfully is reliable and speedy.

Description

technical field [0001] The invention belongs to the technical field of tree endogenous hormones in forestry, and in particular relates to a method for measuring the content of endogenous hormones in tea tree terminal buds based on high performance liquid chromatography (HPLC). Background technique [0002] Tea tree (Camellia sinensis) has a long history of planting in my country and is a traditional economic plant in southern my country (Zhang Yucui, 2010). At present, scientific research on tea trees is mostly focused on morphological indicators, photosynthetic characteristics and intrinsic quality. Through the study of molecular mechanism, it is of great scientific significance to deeply understand the mechanism of plant hormones regulating the growth and development of tea trees and their adaptation to the environment. [0003] Plant hormones (plant hormones, PH) refer to trace organic substances that are synthesized in a certain part of a plant, can be transferred to ot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 杨再强孙擎韩冬
Owner 湖北省鹤峰县翠泉茶业有限公司
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