Microdialysis probe capable of carrying out photogenetic stimulation

Abstract

The invention discloses a microdialysis probe capable of carrying out photogenetic stimulation. The probe comprises a probe shell, a limiting block and an optical fiber connector, the head of the probe shell is connected with a limiting block, the optical fiber connector is combined with the limiting block, the three structures form a main body structure of the probe, an optical fiber module and adialysis module are combined, two operations of optical genetic stimulation and micro dialysis are synchronously carried out, and molecular level change caused after specific neurons are activated byoptical waves can be synchronously detected in real time; the optical fiber can be inserted into a target brain area only when photostimulation and microdialysis operations are carried out, so that neuron damage and tissue reaction caused by a traditional method of placing the optical fiber and the brain for a long time can be avoided, and a long-term tracking experiment is facilitated. The microdialysis probe is used in the field of biomedical instruments.

Description

technical field [0001] The invention relates to the field of biomedical devices, in particular to a microdialysis probe capable of optogenetic stimulation. Background technique [0002] Dialysis is a commonly used technology in the field of life science research. Its main principle is to use semi-permeable membranes to separate small molecules from biological macromolecules, and to perform dialysis and exchange of low-molecular-weight substances that are to be taken out and measured from the perfusate and extracellular fluid, and then collected and analyzed. . Optogenetic technology is currently a cutting-edge technology in the field of neuroscience. Its basic principle is to use genetic manipulation technology to transfect specific types of cells with specific genes that encode light-sensitive proteins, such as ChR2 and NaHR, to express special ion channels. The ion channels under the action of photosensitive proteins will selectively pass through cations or anions under t...

AI Technical Summary

Problems solved by technology

Due to the mechanical properties of the quartz optical fiber, it will cause certain crush damage to the fragile brain tissue, which has a great impact on the experimental results

At present, there is no report on the combination of the front optical fiber of the photostimulation device and microdialysis to achieve simultaneous detection, and this device that can synchronize photostimulation and detection of the concentration of small molecule substances is in great demand for scientific research

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