Direct implantation type microdialysis probe and preparation method thereof

Abstract

The invention relates to a direct implantation type microdialysis probe and a preparation method thereof. The probe comprises a hollow dialysis membrane and two different connecting pipes, wherein thetwo connecting pipes are respectively connected to both sides of the hollow dialysis membrane to be used as a liquid inlet pipe and a liquid outlet pipe, one end of one connecting pipe is burnt intoa pointed head so that the connecting pipe can conveniently penetrate into tissues, and connecting parts are well glued by gluewater; in the process of preparation, one metal wire penetrates through the dialysis membrane and the connecting pipes; when the probe is implanted, the dialysis membrane is supported by the metal wire and can not be bent, folded and broken; and after the probe is implanted, the metal wire is pulled out, the pointed head is nipped off, and perfusate can not be prevented from flowing. The invention has the advantage that the preparation process is simple; and when the probe is used, the thin pointed head penetrates into the tissues, the resistance force is low, and the dialysis membrane is not easily stressed to be bent and folded.

Description

【Technical field】 [0001] The invention relates to the technical field of living body sampling in biomedicine, in particular to the preparation and use method of a directly implanted microdialysis probe. 【Background technique】 [0002] Microdialysis technology is a new trace biochemical sampling and detection technology. The main principle is to use a semi-permeable membrane with a certain molecular weight cut-off to make a microdialysis probe, implant the probe into the target tissue, and the small molecules in the extracellular fluid will expand to the target tissue through the semi-permeable membrane driven by the concentration gradient. In the perfusate, after the effluent with a certain flow rate is collected, high-performance liquid chromatography, capillary electrophoresis and other analytical techniques are used to further measure the information of various substances in the effluent, so as to study the changes of substances in the tissue. [0003] As a living sampli...

AI Technical Summary

Problems solved by technology

Chinese Patent No. 200520046607.X relates to a linear microdialysis probe and its guide tube for biological sampling. The probe and the existing linear probe are composed of a tubular dialysis membrane and a liquid inlet tube and a The outlet pipe is composed of different lengths of the tubular dialysis membrane according to the needs of the experiment, so as to expand the dialysis area to meet the sampling requirements; the two ends of the probe are sealed to prevent the opening from being blocked and scrapped during implantation; the utility model adds a probe guide tube , not only makes it easy to operate during implantation, but also avoids the rupture of the dialysis membrane or the breakage of the probe; however, increasing the outer diameter of the entire device will inevitably cause greater damage to the body tissue

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