Screening method for Chinese-medicine effective ingredient
A screening method and technology of active ingredients, applied in the field of screening of active ingredients of traditional Chinese medicine, can solve the problems of high cost, complex ingredients, unsatisfactory prediction accuracy, etc., and achieve the effect of low cost
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Embodiment 1
[0035] Screening the Active Components in Danggui Buxue Decoction
[0036] Danggui Buxue Decoction consists of two herbs, Angelica and Astragalus, with a clinical ratio of 1:5. Clinically, it is mainly used to treat various anemias. The composition of this prescription is extremely complex, containing polysaccharides, saponins, flavonoids, alkaloids, volatile oils and other components. It is undoubtedly a time-consuming, laborious and inefficient process to prepare them one by one for activity screening. By adopting the screening method of the present invention, the activity of each component can be evaluated through the interaction information of the drug component contained in the prescription and plasma albumin—the protein binding rate, and possible active components can be screened out therefrom.
[0037] Extraction of Danggui Buxue Decoction: Take 5g of crushed Angelica sinensis (commercially available) and 25g of Astragalus membranaceus (commercially available), add 10 ...
Embodiment 2
[0043] Screening the Active Components of Astragalus Extract
[0044] Preparation of Radix Astragali Extract: Take 20 g of crushed Radix Astragali, add 8 times the amount of 90% ethanol to soak for 1 hour, and extract for 1 hour under reflux in a water bath. Use a rotary evaporator to remove the solvent from the extract at 45 ° C, and add 20 ml of pH7.4 to the remaining part. The acetic acid buffer was fully dissolved, centrifuged at 4000rpm / min for 10min, filtered with ordinary filter paper, and stored in a -20°C refrigerator for later use.
[0045] The preparation of bovine plasma albumin (BSA): with embodiment 1
[0046] Microdialysis: Mix 1ml Astragalus extract (1g / ml) and 1ml (1.2mM / L) BSA evenly, let it stand at 25°C for 10 hours, then install the microdialysis device to start sampling, the length of the dialysis membrane is 1cm, and the dialysis The molecular weight cut-off of the membrane is 18KD, the perfusion speed is 1μl / min, and the sampling time is 30min. The dia...
Embodiment 3
[0050] Screening the Active Components of Angelica Extract
[0051] Angelica extract: take 10g of crushed angelica, add 15 times the amount of water to soak for 1h, reflux in an oil bath at 100°C for 1h, remove the solvent with a rotary evaporator at 50°C, and add 5ml of phosphoric acid with a pH of 7.0 to the remaining part The buffer solution was fully dissolved, centrifuged at 4000rpm / min for 10min, filtered with ordinary filter paper, and stored in a -20°C refrigerator for later use.
[0052] The preparation of bovine plasma albumin (BSA): with embodiment 1
[0053] Microdialysis: Mix 1ml Angelica sinensis extract (0.2g / ml) and 1ml (1.2mM / L) BSA evenly, let it stand at 25°C for 10h, then install the microdialysis device to start sampling, the length of the dialysis membrane is 1cm, The molecular weight cut-off of the dialysis membrane is 18KD, the perfusion rate is 1 μl / min, and the sampling time is 30 minutes. The dialysate is collected and analyzed by high performance l...
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