Screening method for Chinese-medicine effective ingredient

A screening method and technology of active ingredients, applied in the field of screening of active ingredients of traditional Chinese medicine, can solve the problems of high cost, complex ingredients, unsatisfactory prediction accuracy, etc., and achieve the effect of low cost

Inactive Publication Date: 2007-04-04
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in vivo pharmacokinetic screening is time-consuming and laborious, the consumption of samples and experimental animals is large, and the research cost is high; and various in vitro pharmacokinetic screening methods are affected by species differences, non-physiological characteristics of in vitro systems and pharmacokinetics in vivo. Due to the influence of dynamics and ot...

Method used

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  • Screening method for Chinese-medicine effective ingredient
  • Screening method for Chinese-medicine effective ingredient
  • Screening method for Chinese-medicine effective ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Screening the Active Components in Danggui Buxue Decoction

[0036] Danggui Buxue Decoction consists of two herbs, Angelica and Astragalus, with a clinical ratio of 1:5. Clinically, it is mainly used to treat various anemias. The composition of this prescription is extremely complex, containing polysaccharides, saponins, flavonoids, alkaloids, volatile oils and other components. It is undoubtedly a time-consuming, laborious and inefficient process to prepare them one by one for activity screening. By adopting the screening method of the present invention, the activity of each component can be evaluated through the interaction information of the drug component contained in the prescription and plasma albumin—the protein binding rate, and possible active components can be screened out therefrom.

[0037] Extraction of Danggui Buxue Decoction: Take 5g of crushed Angelica sinensis (commercially available) and 25g of Astragalus membranaceus (commercially available), add 10 ...

Embodiment 2

[0043] Screening the Active Components of Astragalus Extract

[0044] Preparation of Radix Astragali Extract: Take 20 g of crushed Radix Astragali, add 8 times the amount of 90% ethanol to soak for 1 hour, and extract for 1 hour under reflux in a water bath. Use a rotary evaporator to remove the solvent from the extract at 45 ° C, and add 20 ml of pH7.4 to the remaining part. The acetic acid buffer was fully dissolved, centrifuged at 4000rpm / min for 10min, filtered with ordinary filter paper, and stored in a -20°C refrigerator for later use.

[0045] The preparation of bovine plasma albumin (BSA): with embodiment 1

[0046] Microdialysis: Mix 1ml Astragalus extract (1g / ml) and 1ml (1.2mM / L) BSA evenly, let it stand at 25°C for 10 hours, then install the microdialysis device to start sampling, the length of the dialysis membrane is 1cm, and the dialysis The molecular weight cut-off of the membrane is 18KD, the perfusion speed is 1μl / min, and the sampling time is 30min. The dia...

Embodiment 3

[0050] Screening the Active Components of Angelica Extract

[0051] Angelica extract: take 10g of crushed angelica, add 15 times the amount of water to soak for 1h, reflux in an oil bath at 100°C for 1h, remove the solvent with a rotary evaporator at 50°C, and add 5ml of phosphoric acid with a pH of 7.0 to the remaining part The buffer solution was fully dissolved, centrifuged at 4000rpm / min for 10min, filtered with ordinary filter paper, and stored in a -20°C refrigerator for later use.

[0052] The preparation of bovine plasma albumin (BSA): with embodiment 1

[0053] Microdialysis: Mix 1ml Angelica sinensis extract (0.2g / ml) and 1ml (1.2mM / L) BSA evenly, let it stand at 25°C for 10h, then install the microdialysis device to start sampling, the length of the dialysis membrane is 1cm, The molecular weight cut-off of the dialysis membrane is 18KD, the perfusion rate is 1 μl / min, and the sampling time is 30 minutes. The dialysate is collected and analyzed by high performance l...

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Abstract

A method for screening the active components of Chinese-medicinal material includes such steps as mixing the liquid extract of the Chinese-medicinal material to be screened with serum albumin solution, sampling by equilibrium dialysis or microdialysis to obtain reactive dialytic liquid, mixing the liquid extract of the Chinese-medicinal material to be screened with the blank solution not containing serum albumin, sampling by equilibrium dialysis or microdialysis to obtain blank dialytic liquid, respective efficient liquid-phase chromatography analysis for said reactive dialytic liquid and blank dialytic liquid, and calculating the albumin combining percentage of each component.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to a screening method for active components of traditional Chinese medicines, specifically to a method for screening activity of different components by establishing interaction information between components of traditional Chinese medicines and proteins. Background technique [0002] The screening of active ingredients of traditional Chinese medicine usually uses phytochemical methods to extract the effective part compound group or monomer compound, and then confirms it through pharmacological experiments. However, due to the extremely complex chemical components in traditional Chinese medicine and its compound, traditional screening methods need to consume a large number of samples and experimental animals, and the screening efficiency is not high. Modern drug screening not only screens effective active compounds through specific targets, but also screens compounds through pharmac...

Claims

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Application Information

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IPC IPC(8): A61K36/00G01N33/68
Inventor 李萍闻晓东齐练文刘承伟
Owner CHINA PHARM UNIV
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