The invention discloses a chronic continuous
drug delivery method in the
ventricle of an
experimental animal, and belongs to the technical field of
animal model construction. The method comprises the following steps: (1) anesthetizing an animal; (2) longitudinally
cutting the
skin of the
parietal bone along the direction of the
occipital bone macropore formed by the midpoint of the connecting line of the two eyes; (3) disinfecting the head and removing connective tissues on the surface of the
parietal bone; (4) penetrating through the left
ventricle by using a brain stereotaxic instrument; (5) simultaneously connecting a chronic
drug delivery pump with a
catheter, soaking the chronic
drug delivery pump in normal
saline for 20-28 hours, taking out the chronic
drug delivery pump, filling the chronic
drug delivery pump with a tested drug, and injecting the tested drug into the
catheter; (6)
cutting the
catheter to a
proper length, inserting the far end of the catheter into the left
ventricle, fixing, and after the catheter is dried up, remaining the chronic
drug delivery pump under the
skin of the head along the opening of the
skin of the head; and (7) sewing a head opening. According to the method, insufficient
drug effect persistence caused by too high
drug metabolism speed can be avoided, and in addition, injury to the tested animal due to multiple times of opening and
puncturing can be reduced, so that non-drug influence is reduced.