289 results about "Endoscopic imaging" patented technology

The invention relates to the technical field of medical instruments, and discloses a laparoscope and digestive endoscope combined positioning measurement system and method.The method comprises the steps of positioning mark initialization, image feature analysis, instrument path planning, dynamic positioning calibration, operation integrity evaluation and the like. Spatial matching is realized by setting multiple types of positioning marks and utilizing optical reflection signals, noise reduction and feature extraction are performed on endoscope imaging data to generate a measurement area boundary, an operation path is planned in combination with anatomical path information, the tail end position of an instrument is calibrated in real time, and operation integrity is evaluated. The system comprises a positioning module, an analysis module, a correction module and an integration module which are respectively used for realizing the functions of positioning mark setting, image processing and path generation, offset correction and operation evaluation. According to the system, the operation positioning precision and the operation safety are improved, intellectualization and precision of minimally invasive surgery are achieved, and the system is suitable for minimally invasive treatment of digestive system diseases.

The application discloses a multi-marked endoscopic imaging lens for digestive tract, and belongs to the field of biomedical microscopic endoscopic imaging. The endoscopic imaging lens comprises a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens and a seventh lens; the first lens focuses laser; the second lens is matched with the first lens to limit a light beam in an effective light aperture of the lens; the third lens corrects chromatic aberration; the fourth lens and the fifth lens are combined to correct aberration; the sixth lens adopts an aspheric lens to correct spherical aberration caused in a collimating and focusing system; and the seventh lens is matched with the sixth lens to correct chromatic aberration. The working spectral range of the microscopic objective lens group is 488-860 nm, the effective light aperture is less than 1.8 mm, the mechanical outer diameter is not greater than 2.6 mm, the numerical aperture at the object side or the tissue is 0.55, the imaging field of view is 450 microns, the magnification is 2.14 times, and the working distance is 84 microns. The effect of matching excitation and fluorescence spectrum of a clinically available fluorescence contrast agent is realized.

The invention discloses an ultra-wide-spectrum endoscopic imaging device capable of being used for short-wave infrared fluorescence imaging, which is used for in-vivo minimally invasive imaging and comprises an illumination module, a wide-spectrum image transmission module, an imaging module and an image processing module. The illumination module consists of a light source and a light guide optical fiber, and is used for generating illumination light from ultraviolet to short wave infrared band and transmitting the illumination light to the wide-spectrum image transmission module; the wide-spectrum image transmission module consists of a few-interface optical imaging channel and a light guide illumination channel, and is used for illuminating an imaging target, exciting target fluorescence and collecting image signals from ultraviolet to short-wave infrared bands; the imaging module is composed of an optical filter and an imaging camera, and the imaging camera can be a wide spectral response image sensor or can be formed by combining cameras working in different wavebands and is used for converting optical images in different wavebands into image data; and the image processing module is used for registering and fusing image data of different wavebands in real time and displaying the image data.

The invention discloses a multi-photon optical fiber array endoscopic imaging device and method based on hollow spiral scanning, and belongs to the technical field of biological imaging. The device comprises a laser collimation and beam expansion module, a pulse chirp module, a light beam switching module, an endoscopic detection module and a fluorescence acquisition module. The light beam switching module comprises an optical switch and an optical fiber array composed of at least two low-group-velocity dispersion single-mode optical fibers. The endoscopic detection module drives the optical fiber array to execute hollow spiral scanning through the resonance driver, the track starting point is not located at the view field original point, and central dense sampling is avoided. According to the method, the optical fibers are switched through the optical switch, so that the optical fibers execute spatial non-coincident hollow spiral scanning, multi-photon signals such as fluorescence, second harmonic and fluorescence lifetime are acquired, a central hole is complemented through image reconstruction and fusion, and a complete field imaging result is obtained. Light damage of the central area is remarkably reduced, the imaging resolution uniformity is improved, the fused image is true and reliable based on physical sampling, and the system stability is high.

An endoscopic imaging system for use in a light deficient environment includes an imaging device having a tube, one or more image sensors, and a lens assembly including at least one optical elements that corresponds to the one or more image sensors. The endoscopic system includes a display for a user to visualize a scene and an image signal processing controller. The endoscopic system includes a light engine having an illumination source generating one or more pulses of electromagnetic radiation and a lumen transmitting one or more pulses of electromagnetic radiation to a distal tip of an endoscope.

The present invention relates to an endoscopic probe for photoacoustic tomography of cavity side walls, characterized by comprising a hollow tubular probe housing with a closed front end; N acoustic wave receiving windows and M laser output windows formed on the side wall of the tubular probe housing; an array of linear laser output ports connected to a pluggable optical fiber connector via an optical fiber bundle; and an ultrasonic transducer array connected to a multi-channel signal connector via a signal harness. The present invention employs a method of arranging array transducers and array laser outputs on the side wall of the probe, eliminating the need for a reflector. The probe is less than 2 centimeters in size and can be placed in close proximity to the prostate through the rectum for photoacoustic imaging of the prostate, or in close proximity to the inner wall of the rectum for photoacoustic imaging of the inner wall of the rectum. The endoscopic probe provided by the present invention, combined with a photoacoustic imaging system host, utilizes photoacoustic endoscopic imaging technology to perform real-time transrectal prostate imaging and rectal imaging, achieving ideal imaging results.

The invention discloses an optical fiber coupling real-time correction method in a single-mode and multi-mode optical fiber endoscopic imaging system, which comprises the following steps of: firstly, manually adjusting reflectors M1 and M2 to an optimal multi-mode optical fiber coupling state, acquiring images of light spots at the near end and the far end of a laser beam in the state by using a CCD (charge coupled device) 1 and a CCD 2, and calculating the position of a central coordinate of the images; and collecting a speckle image formed by the light spot through the scattering medium by using a CCD3 (charge coupled device 3). The system monitors the center coordinates of near-end and far-end light spots at the current moment and the correlation coefficient of the current speckle image and the collimation state image in real time. The piezoelectric mirror bracket is fed back and adjusted in real time through the center coordinates of the two spot light spots and the correlation coefficient of the speckles, so that the correlation coefficient of the speckles is kept above a set threshold value, and automatic calibration of light beams is achieved. On the basis of the traditional thinking that two points determine one straight line, the correlation coefficient of using speckles is increased, the alignment precision is improved, and the method has certain practicability and application prospects.

The invention relates to the technical field of image processing, and discloses an endoscope image imaging method and an endoscope, and the endoscope image imaging method comprises the steps: obtaining a real-time collection image of the endoscope and a light source parameter of the endoscope; positioning a target light spot in the real-time acquired image based on light source parameters of the endoscope; suppressing a specular reflection component of a target area where the target light spot is located to obtain an endoscope imaging image; the method comprises the following steps: firstly, positioning a light spot in a real-time acquired image based on a light source parameter to obtain a target light spot; the specular reflection component of the target area where the target light spot is located is further suppressed, so that the overexposure spots can be suppressed while global processing damage to normal highlight areas such as highlight bleeding spots and highlight mucous membranes can be avoided, and the original expression of the image can be restored.

The invention provides an endoscope imaging system and a hard tube type endoscope, and belongs to the technical field of optical imaging, the endoscope imaging system comprises an objective lens, a relay lens and an ocular lens of an object space telecentric structure, the objective lens comprises a first lens, a second lens, a third lens with positive focal power and a bonding lens group with positive focal power, the second lens is a steering prism, the image side surface and the object side surface of the first lens are aspheric surfaces, the balsaming lens group comprises a fourth lens, a fifth lens and a sixth lens, the fourth lens is a doublet lens, the fifth lens is a triplet lens, the sixth lens is a doublet lens, the relay lens comprises an odd number of rod lens groups which are arranged at intervals, and the rod lens groups are arranged at intervals. The eyepiece comprises a seventh lens, an eighth lens and a ninth lens, and the seventh lens, the eighth lens and the ninth lens are all doublet lenses. By optimizing the structural design of the optical system, the defocusing amount is reduced, seamless switching of visible light and near-infrared light dual-mode imaging can be achieved, and meanwhile high-quality imaging is achieved.

The invention relates to an endoscope imaging system and a light source thereof, the light source comprises a laser light source, a visible light source and a light combiner, the laser light source is used for outputting laser of at least two different wavebands, the laser of the different wavebands is used for exciting different fluorescent dyes, and the visible light source is used for outputting visible light; the light combiner is used for combining the laser and the visible light into a combined light beam and outputting the combined light beam to the endoscope; wherein the laser light source is a single light-emitting device, the single light-emitting device comprises a single substrate and at least two laser chips arranged on the single substrate, the at least two laser chips are used for emitting laser of different wave bands, and the single light-emitting device further comprises a single light outlet and a single optical fiber; and laser emitted by the at least two laser chips is output to the single optical fiber through the single light outlet. The light source can independently or simultaneously output laser of different wave bands to excite different fluorescent dyes, and the layout of an endoscope imaging system is simplified by adopting the light source.

The invention relates to the technical field of endoscope imaging, and discloses a depth-of-field continuation imaging device and method for an endoscope and electronic equipment. The microlens array is composed of a plurality of microlenses with positive focal power, the microlens array is used for obtaining an original image collected by the endoscope, a light field image comprising sub-aperture images corresponding to the microlenses is obtained, depth calculation is carried out according to the parallax value between the different sub-aperture images, and the depth of the endoscope is calculated according to the parallax value between the sub-aperture images. Obtaining a pixel depth corresponding to an image pixel in the original image, refocusing each sub-aperture image on a focal plane corresponding to the pixel depth to obtain a refocused image corresponding to each sub-aperture image, and determining a definition weight corresponding to the image pixel from each refocused image; and carrying out image fusion on the pixel regions of the image pixels in the corresponding definition weights to obtain a target image. Compared with depth-of-field continuation according to multiple frames of images, the target picture with clear texture and extended depth-of-field can be obtained, and the image quality is improved.

The present invention discloses a fiber-optic ultrasonic endoscope with integrated lateral transmission and reception and a preparation method, which belongs to the field of ultrasonic endoscopic imaging. It comprises a double-clad optical fiber, an excitation film, a dielectric mirror, and a sensing film. By etching part of the inner cladding of the optical fiber into a concave spherical surface and coating it with an excitation film, the excitation light transmitted in the inner cladding is absorbed and focused ultrasonic waves transmitted laterally are excited. By etching part of the inner cladding and the core of the optical fiber and preparing it into a Fabry-Perot (F-P) cavity. When the ultrasonic signal transmitted laterally into the ultrasonic endoscope acts on the sensing film of the cavity, the effective refractive index of the sensing film and the F-P cavity length change, and the signal light in the core demodulates this change to complete the detection of ultrasonic waves. The present invention etches the inner cladding and coats it with an excitation film to achieve the excitation of laterally focused ultrasonic waves, etches an F-P cavity on the side of the optical fiber to achieve the lateral detection of ultrasonic waves, and thus forms a fiber-optic ultrasonic endoscope with integrated lateral transmission and reception.

The invention discloses a self-adaptive intervention type single-array-element photoacoustic endoscopic imaging near-field noise removal method based on a spatial domain and morphological characteristics. The method comprises the following steps that 1, original data of photoacoustic endoscopic imaging are obtained; step 2, adaptively generating a patch mask area; 3, preprocessing the image in a frequency domain; step 4, executing strip interference detection based on adaptive spatial domain filtering on the non-plaque region to obtain a spatial domain noise mask; step 5, screening strip-shaped noise areas; step 6, performing interpolation restoration on the noise area; 7, calculating the convergence degree and the noise level in the iteration process; and step 8, outputting a final result after polar coordinate transformation. The intrusive single-array-element photoacoustic endoscopic imaging method can effectively inhibit strip-shaped interference signals occurring in intrusive single-array-element photoacoustic endoscopic imaging and reduce the covering influence of background noise on target signals, so that the imaging quality of a near-end area is improved, and the structural definition and contrast ratio of overall imaging are remarkably optimized.

There is provided a processor device that performs a type determination method for an endoscope by a processor using an endoscope color adjustment jig, the endoscope color adjustment jig including an insertion portion that includes at least one insertion hole into which a distal end portion of the endoscope is inserted, a lid portion that is disposed to face the insertion portion in an insertion direction of the distal end portion, and an outer peripheral portion that is in contact with the insertion portion and the lid portion to form an internal space, the lid portion including a chart that includes a mark on an inner surface facing the internal space, in which the processor is configured to, based on a shape of the mark imaged by the endoscope inserted from the insertion portion, determine an oblique angle of the endoscope.

The invention discloses a balloon type heart cavity endoscope imaging system and method based on a three-dimensional mapping system, and belongs to the technical field of medical equipment. The problems that in an existing imaging method, imaging in the heart cavity is interfered by blood, the image definition is insufficient, and intuitive visualization of an inner wall image cannot be achieved are solved. A filling opening is formed in the handle end of the balloon type heart cavity endoscope, the filling opening is used for injecting liquid into a balloon so that the balloon can expand to be attached to the inner wall of a heart cavity to drain blood at the attached position of the inner wall of the heart cavity, and an image sensor is used for conducting image collection on the tissue, attached to the outside of the balloon, of the inner wall of the heart cavity; the positioning assembly is used for acquiring the position and posture of the image sensor in real time; the image processing unit is used for preprocessing the image acquired by the image sensor and then transmitting the image to the three-dimensional mapping system; the three-dimensional mapping system establishes a mapping heart cavity model, and the collected image and the mapping heart cavity model are matched, fused and displayed. The method is mainly used for intracardiac imaging.

The invention discloses an endoscope imaging method and system. The method comprises the following steps: acquiring a real-time acquired image of an endoscope; performing brightness clustering on the real-time acquired image, extracting an area with a brightness value in a front preset proportion as an initial light spot candidate area, and performing circularity detection on the initial light spot candidate area to select a light spot candidate area from the initial light spot candidate area; extracting an LBP local texture histogram of the light spot candidate region to obtain a corresponding texture entropy, and determining a target light spot region from the light spot candidate region according to a size relationship between the texture entropy and a texture entropy threshold value; and in the real-time acquired image, inhibiting factors which influence the image quality and are caused by the target light spot in the target area where the target light spot area is located, and obtaining an endoscope imaging image. The problem that in the prior art, the imaging quality of an endoscope is low is solved.

The invention discloses a device for measuring photoacoustic endoscopic imaging resolution and depth, and belongs to the technical field of biomedical imaging detection.The device comprises a vertical plate, a height adjusting mechanism is arranged on the vertical plate, the height adjusting mechanism is connected with a liftable probe clamping assembly, and a bottom plate is fixedly arranged at one end of the vertical plate; a scanning imaging rotating motor is arranged on one side of the bottom plate, the output end of the scanning imaging rotating motor is connected with a coupling medium container, a base body is installed in the coupling medium container, a plurality of through holes distributed in an Archimedes spiral shape are formed in the base body, and a plurality of target wires used for providing photoacoustic signal comparison are arranged in the through holes in a penetrating mode. According to the invention, a complex tissue environment can be simulated, and a multi-azimuth and multi-scale imaging result can be obtained, so that the resolution ratio and the imaging depth of the photoacoustic endoscopic system can be evaluated, and the method is suitable for detection and standardized evaluation of different types of photoacoustic endoscopic equipment.

The invention discloses a method for identifying a blood vessel in an endoscope image and an endoscope system. The method for identifying the blood vessel in the endoscope image comprises the following steps: acquiring a multi-channel image formed by endoscope imaging; time sequence points of all points in all RGB channels in the to-be-analyzed area in the multi-channel image are extracted, and normalization processing is carried out; according to the time sequence point of each point in each RGB channel in the to-be-analyzed area, respectively calculating the time sequence point of the mean value of each RGB channel in the to-be-analyzed area, and carrying out normalization processing; according to the normalized time sequence point of each point in each RGB channel in the to-be-analyzed area, determining a PPG amplitude for representing blood fluctuation information at each point; determining an average amplitude for representing blood fluctuation information according to a time sequence point of an average value of each RGB channel in the normalized region to be analyzed; and determining a blood vessel region according to the PPG amplitude of each point, the average amplitude, the penetration depth of the light sources with different wavelengths to human tissues and the difference of the absorption characteristics of blood components.

The present application relates to the field of medical equipment, and in particular to an endoscopic imaging device, an imaging system, and a method thereof having a diagnostic imaging device. The device comprises: a diagnostic imaging device, an external control device, and an ingestible capsule endoscope. The external control device is used to control the position and / or posture of the capsule endoscope within a target area; the diagnostic imaging device is disposed within the capsule endoscope and is used to perform diagnostic imaging of the target area under the control of the external control device. The endoscopic imaging device and the imaging method thereof provided in the present application utilize a diagnostic imaging device to examine the target area without the need for a tissue biopsy, thereby not only improving the accuracy of the examination of the lesion area, but also reducing the risk of infection in the patient.

The invention discloses a bending endoscopic imaging method and system based on a few-mode optical fiber, and belongs to the technical field of optical fiber imaging. According to the method, an MNIST handwritten digital image is loaded through a digital micromirror device (DMD), a speckle pattern is generated through few-mode optical fiber transmission, image restoration in a bending state is achieved through a deep learning model, and reconstruction effects under different bending degrees are quantized through a Dice coefficient. The system comprises a light beam collimation module, a pattern loading module, an image transmission module and an image acquisition-processing module. The image transmission module adopts a step refractive index few-mode optical fiber, and mode coupling caused by bending is inhibited by increasing the effective refractive index difference between modes; and the image acquisition-processing module is combined with a shallow U-shaped convolutional neural network to reconstruct a speckle pattern acquired by the CCD camera. While miniaturization of the probe is ensured, the imaging quality in a bending environment is remarkably improved, and the probe is suitable for miniaturized high-resolution imaging scenes such as medical endoscopes and the like.

The application provides an endoscopic imaging system field mask obtaining method and device, wherein the endoscopic imaging system field mask obtaining method comprises the following steps: firstly, imaging a target object to obtain a target image; then, determining the positions of feature pixel points in the target image according to the pixel values of the target image; then, forming a binary image according to the positions of the feature pixel points; finally, performing morphological processing on the binary image to obtain an endoscopic imaging system field mask. Through the endoscopic imaging system field mask obtaining method, the circular window region of the fiber bundle end face can be accurately identified, and the endoscopic imaging system field mask can be obtained at a high speed. The obtained endoscopic imaging system field mask has a good shielding effect on the region outside the circular window of the fiber bundle end face, and is convenient for diagnosing the body tissue lesion condition of a subject.

The invention discloses an endoscope imaging optimization method, and belongs to the technical field of medical optical imaging and image processing. The method comprises the following steps: firstly, obtaining a super-structure lens distortion image, a high-definition truth value image and a semantic segmentation label; extracting multi-scale initial features through a multi-branch encoder; carrying out feature enhancement and self-adaptive downsampling by utilizing a dynamic super-surface feature extraction module; constructing robust features through a dynamic parameter generation module in combination with the high-halal truth value image; then, extracting contour guide features from the high-halal truth value image and fusing the contour guide features with robust features; and finally, image segmentation and recovery tasks are executed in parallel, and a segmentation mask and a distorted image are output. By fusing physical prior and data driving, the problems of chromatic aberration, information dispersion and the like of super-structure lens imaging are effectively solved, and the image definition, the small focus recognition precision and the model generalization ability are remarkably improved.

The invention relates to the technical field of endoscope imaging, in particular to an endoscope fault detection and processing method and system. According to the scheme, firstly, a current image frame in a video shot by a camera of an endoscope is obtained; then, inputting the obtained current image frame into a pre-trained image discrimination neural network model for judgment, and finally outputting a classification type and confidence corresponding to the image information; and then, according to the classification type and the confidence coefficient, judging whether an image abnormal fault occurs in the endoscope, if the image abnormal fault occurs, determining a corresponding fault processing measure according to the classification type, performing corresponding processing on the endoscope according to the determined fault processing measure, and if the image abnormal fault does not occur, performing corresponding processing on the endoscope. And if so, continuing to acquire the next image frame. By adopting the scheme of the invention, real-time fault judgment can be carried out, and adaptive judgment and correction can be carried out on possible image abnormity, so that the endoscope fault can be effectively prevented from being continued or expanded.

The invention relates to a method for analysing images of biological tissues acquired by endoscopy, using a light-generating device associated with an endoscope with a camera.

A method for guiding bone removal includes receiving at least one two-dimensional image of bony anatomy captured by a two-dimensional imaging system and at least one endoscopic image of the bony anatomy captured by an endoscopic imager. A three-dimensional model of the bony anatomy is registered with the endoscopic imager in a common reference frame using the two-dimensional image and a tracking system. A position and orientation of the three-dimensional model relative to the endoscopic image is determined based on registration of the three-dimensional model with the endoscopic imager in the common reference frame. The endoscopic image is displayed simultaneously with at least a portion of the three-dimensional model based on the determined position and orientation of the three-dimensional model relative to the endoscopic image.

The present application belongs to the technical field of endoscope and image processing, and in particular to an endoscope image anti-shake method based on motion vector compensation, comprising the following steps: collecting a sequence of continuous video frames, performing stability screening on the initial feature point set, eliminating unstable feature points located in the edge region, high light reflection region and low contrast region of the image to form a reliable feature point set, performing robust estimation processing on the original motion vector set based on the matched feature points in the reliable feature point set to generate a stabilized output frame, wherein the remapping adopts a bilinear interpolation algorithm; by constructing a closed-loop anti-shake system taking local feature motion vectors as input, spatio-temporal domain joint filtering as core and sub-pixel remapping as output, high-precision separation and compensation of high-frequency micro-shaking in endoscope imaging are realized without introducing additional mechanical structures.

The embodiment of the specification relates to the technical field of super-resolution fluorescence microscopy, and particularly relates to an optical superlens optical sheet endoscopy imaging system and method. Phase control is performed on short-wavelength laser by an optical sheet superlens, so that incident light on a sample is finely adjusted into the form of an optical sheet, the short-wavelength laser after phase control is irradiated on the sample, the optical sheet and the sample interact to excite fluorescence, at the same time, phase control is performed on long-wavelength laser by an OAM light superlens, the obtained OAM light is irradiated on the sample, the OAM light quenches fluorescent substances at a specified position in a fluorescent spot excited by the short-wavelength laser, so that the diffraction area of the fluorescent spot is reduced, finally, fluorescence remaining after OAM light irradiation of the sample is converged on a photodiode for fluorescence power detection, and the resolution of imaging is improved on the basis of scanning of samples such as living cells.

The application relates to a kind of self-adapting scattering deep penetration microscopic endoscopic imaging method, device and medium, method comprises the following steps: S1, the input-output mapping relationship of optical fiber under specified penetration depth is measured;S2, when the same specified penetration depth is set with biological sample, effective pixel is traversed;S3, when traversing each effective pixel, the traversal range of the phase constant of each pixel is adjusted, and the phase constant corresponding to each pixel is obtained, so that the light intensity is maximum;S4, data integration is carried out, and a group of self-adapting scattering matrix corresponding to each pixel is obtained;S5, calling self-adapting scattering matrix as phase parameter, scanning is carried out pixel by pixel, and the light intensity corresponding to pixel is obtained, and the light intensity is combined into a frame gray scale image.Compared with prior art, the application has the advantages of clearly imaging deep tissue, effectively improving imaging quality and the like.

The utility model relates to a device for testing the imaging performance of a fluorescent endoscope, which comprises a mounting frame, and a clamp assembly, a container placing disc and an integrating sphere which are sequentially distributed on the mounting frame from top to bottom, and a lighting device arranged on one side of the integrating sphere, and exciting light of the lighting device illuminates the container placing disc through the integrating sphere. The container placing disc is provided with observation holes which are distributed in a circumferential mode, the clamp assembly comprises a double-screw clamp used for fixing the fluorescent endoscope and an adjusting frame used for adjusting the irradiation angle of the fluorescent endoscope, and the main purpose of the utility model is to enable a light source in the container placing disc to be more uniform through the reflection effect of an integrating sphere; according to the fluorescent endoscope, the sensitivity and definition of the fluorescent endoscope are improved, three important indexes including the radiance response characteristic, the fluorescent signal-to-noise ratio and the fluorescent static image latitude of the fluorescent endoscope are analyzed through real-time data of a fluorescent image collected by the fluorescent endoscope, and important parameters are provided for judging the imaging performance of the fluorescent endoscope.

The application discloses a kind of multi-photon fiber array endoscopic imaging device and method based on hollow spiral scanning, belong to biological imaging technical field.The device includes laser collimation and beam expansion module, pulse chirp module, beam switching module, endoscopic detection module and fluorescence acquisition module.The beam switching module contains optical switch and at least two low group velocity dispersion single-mode fiber array;Endoscopic detection module drives fiber array to perform hollow spiral scanning by resonant driver, its track starting point is not located at the origin of field of view, to avoid central dense sampling.The method switches fiber by optical switch, so that each fiber performs spatial non-overlapping hollow spiral scanning, collects multi-photon signals such as fluorescence, second harmonic and fluorescence lifetime, and completes the central cavity by image reconstruction and fusion, to obtain complete field of view imaging result.The application significantly reduces the central region light damage, improves the uniformity of imaging resolution, and the fused image is based on physical sampling to ensure authenticity and reliability, with high system stability.