Industrial production process for fast separating and purifying ganglioside by using chromatography media having special identification function

A technology of gangliosides and chromatographic media, which is applied in the field of industrial production technology, can solve the problems of lack of treatment measures and high morbidity, and achieve the effects of shortening the separation and purification time, simple operation, and increasing yield

Active Publication Date: 2015-02-25
李桂凤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the incidence of neurological diseases is very high and there is often a lack of effective treatment measures. New drugs such as gangliosides with definite curative effects and few side effects have a wide market demand and urgently need to be researched and developed.

Method used

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  • Industrial production process for fast separating and purifying ganglioside by using chromatography media having special identification function
  • Industrial production process for fast separating and purifying ganglioside by using chromatography media having special identification function
  • Industrial production process for fast separating and purifying ganglioside by using chromatography media having special identification function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Take pig fresh brain tissue as an example for illustration.

[0030] 1) Development of chromatographic medium with specific recognition function:

[0031] Pretreatment of medical stone: add medical stone with a particle size of 20-60 mesh into 1mol / L hydrochloric acid, the mass ratio of medical stone to hydrochloric acid solution is 1:12; soaking time is 30 hours; remove organic matter and other impurities, After soaking, wash the medical stone with distilled water until it is neutral, and dry it for later use;

[0032] Preparation of chromatographic medium with specific recognition function: first add chitosan with acetylation degree of 75% to acetic acid solution with a concentration of 6%, and prepare 60ml of chitosan solution with a concentration of 0.3%; Add 20g of medical stone into the chitosan solution to make a paste, make it fully infiltrated, put the paste in a microwave oven, heat and dry, grind it through a 100-mesh sieve, and then get a chro...

Embodiment 2

[0039] Embodiment 2: Take fresh bovine brain tissue as an example for illustration.

[0040] 1) Development of chromatographic medium with specific recognition function:

[0041]Pretreatment of medical stone: add medical stone with a particle size of 20-60 mesh into 2.5mol / L hydrochloric acid, the mass ratio of medical stone to hydrochloric acid solution is 1:15; soaking time is 38 hours; remove organic matter and other impurities , after soaking, wash the medical stone with distilled water until it is neutral, and dry it for later use;

[0042] Preparation of chromatographic medium with specific recognition function: first add chitosan with an acetylation degree of 90% to an acetic acid solution with a concentration of 8%, and prepare 120ml of a chitosan solution with a concentration of 0.5%; Add 50g of medical stone into the chitosan solution to make a paste, fully infiltrate it, put the paste in a microwave oven, heat and dry, grind and pass through a 120-mesh sieve to obt...

Embodiment 3

[0049] Embodiment 3: Take fresh sheep brain tissue as an example for illustration.

[0050] 1) Development of chromatographic medium with specific recognition function:

[0051] Pretreatment of medical stone: add medical stone with a particle size of 20-60 mesh into 0.5mol / L hydrochloric acid, the mass ratio of medical stone to hydrochloric acid solution is 1:10; soaking time is 20 hours; remove organic matter and other impurities , after soaking, wash the medical stone with distilled water until it is neutral, and dry it for later use;

[0052] Preparation of chromatographic medium with specific recognition function: first add chitosan with 60% acetylation to 1% acetic acid solution to prepare 180ml of chitosan solution with 0.1% concentration; Add 80g of medical stone into the chitosan solution to make a paste, fully infiltrate it, put the paste in a microwave oven, heat and dry, and grind it through a 90 mesh sieve to obtain a chromatographic medium with a specific recogni...

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PUM

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Abstract

The invention provides an industrial production process for fast separating and purifying ganglioside by using chromatography media having a special identification function. The process comprises three steps of static adsorption, elution, and concentration and drying, wherein the step of static adsorption comprises the steps of: adding extracting solution into animal brain tissue for homogenation, centrifugation and demixing, directly adding supernatant liquid subjected to the demixing into the chromatography media having a special identification function, stirring for absorption for 1 to 2 hours and fully stirring and mixing the chromatography media having the special identification function and the extracting solution; the step of elution comprises the step of directly adding the chromatography media having the special identification function and absorbing the ganglioside into elution liquid for elution for 1 to 2 hours; and the step of concentration and drying comprises the steps of: concentrating the elution liquid and then refrigerating and drying the concentrated elution liquid in the environment of -70 to 20 DEG C to obtain the ganglioside product. In the process, the chromatography media and the extracting solution are fully stirred and mixed by using the methods of static absorption and elution to minimize the loss of the ganglioside in the extracting process; compared with the conventional macroporous resin chromatography, the process has the advantages that: the yield is increased by 15 to 20 percent, the separation and purification time is shortened by 10 to 38 times; moreover, the process is simple to operate and favorable for industrial production.

Description

(1) Technical field [0001] The invention relates to an industrialized production process of gangliosides with biological activity, in particular to an industrialized production process for rapid separation and purification of gangliosides with a chromatographic medium with a specific recognition function. (2) Technical background [0002] Gangliosides have very important biological functions. They are glycolipid complexes with small molecular weight. They can enter the brain through the blood-brain barrier, embedded in damaged nerve cell membranes, repair Damaged nerves. It is an internationally recognized nutrient and growth factor that can directly act on human brain cells, that is, the nervous system. It can treat nerve damage and promote nerve regeneration. It is effective for different periods, different reasons, and different degrees of nerve damage, degeneration, and aging. Restorative effect. It has four major medical functions: promoting the normal development of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/10C07H1/08B01D15/08B01D15/42
Inventor 李桂凤李宗霖李传胜万春燕
Owner 李桂凤
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